Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis
Estrogen affects endometrial cellular proliferation by regulating the expression of the <i>c-myc</i> gene. B-cell translocation gene 1 (BTG1), a translocation partner of the <i>c-myc</i>, is a tumor suppressor gene that promotes apoptosis and negatively regulates cellular pro...
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doaj-99d0efedf24648a78145f3572d187c4b2020-11-25T00:06:23ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-07-012013337210.3390/ijms20133372ijms20133372Role of B-Cell Translocation Gene 1 in the Pathogenesis of EndometriosisJeong Sook Kim0Young Sik Choi1Ji Hyun Park2Jisun Yun3Soohyun Kim4Jae Hoon Lee5Bo Hyon Yun6Joo Hyun Park7Seok Kyo Seo8SiHyun Cho9Hyun-Soo Kim10Byung Seok Lee11Department of Obstetrics and Gynecology, University of Ulsan College of Medicine, Ulsan University Hospital, Ulsan 44033, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Obstetrics and Gynecology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul 06273, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaInstitute of Women’s Life Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaInstitute of Women’s Life Medical Science, Yonsei University College of Medicine, Seoul 03722, KoreaDepartment of Pathology and Translational Genomics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, KoreaDepartment of Obstetrics and Gynecology, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, KoreaEstrogen affects endometrial cellular proliferation by regulating the expression of the <i>c-myc</i> gene. B-cell translocation gene 1 (BTG1), a translocation partner of the <i>c-myc</i>, is a tumor suppressor gene that promotes apoptosis and negatively regulates cellular proliferation and cell-to-cell adhesion. The aim of this study was to determine the role of BTG1 in the pathogenesis of endometriosis. <i>BTG1</i> mRNA and protein expression was evaluated in eutopic and ectopic endometrium of 30 patients with endometriosis (endometriosis group), and in eutopic endometrium of 22 patients without endometriosis (control group). The effect of BTG1 downregulation on cellular migration, proliferation, and apoptosis was evaluated using transfection of primarily cultured human endometrial stromal cells (HESCs) with <i>BTG1</i> siRNA. <i>BTG1</i> mRNA expression level of eutopic and ectopic endometrium of endometriosis group were significantly lower than that of the eutopic endometrium of the control group. Migration and wound healing assays revealed that BTG1 downregulation resulted in a significant increase in migration potential of HESCs, characterized by increased expression of matrix metalloproteinase 2 (MMP2) and MMP9. Downregulation of BTG1 in HESCs significantly reduced Caspase 3 expression, indicating a decrease in apoptotic potential. In conclusion, our data suggest that downregulation of BTG1 plays an important role in the pathogenesis of endometriosis.https://www.mdpi.com/1422-0067/20/13/3372BTG1endometriosishuman endometrial stromal cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeong Sook Kim Young Sik Choi Ji Hyun Park Jisun Yun Soohyun Kim Jae Hoon Lee Bo Hyon Yun Joo Hyun Park Seok Kyo Seo SiHyun Cho Hyun-Soo Kim Byung Seok Lee |
spellingShingle |
Jeong Sook Kim Young Sik Choi Ji Hyun Park Jisun Yun Soohyun Kim Jae Hoon Lee Bo Hyon Yun Joo Hyun Park Seok Kyo Seo SiHyun Cho Hyun-Soo Kim Byung Seok Lee Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis International Journal of Molecular Sciences BTG1 endometriosis human endometrial stromal cells |
author_facet |
Jeong Sook Kim Young Sik Choi Ji Hyun Park Jisun Yun Soohyun Kim Jae Hoon Lee Bo Hyon Yun Joo Hyun Park Seok Kyo Seo SiHyun Cho Hyun-Soo Kim Byung Seok Lee |
author_sort |
Jeong Sook Kim |
title |
Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis |
title_short |
Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis |
title_full |
Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis |
title_fullStr |
Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis |
title_full_unstemmed |
Role of B-Cell Translocation Gene 1 in the Pathogenesis of Endometriosis |
title_sort |
role of b-cell translocation gene 1 in the pathogenesis of endometriosis |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-07-01 |
description |
Estrogen affects endometrial cellular proliferation by regulating the expression of the <i>c-myc</i> gene. B-cell translocation gene 1 (BTG1), a translocation partner of the <i>c-myc</i>, is a tumor suppressor gene that promotes apoptosis and negatively regulates cellular proliferation and cell-to-cell adhesion. The aim of this study was to determine the role of BTG1 in the pathogenesis of endometriosis. <i>BTG1</i> mRNA and protein expression was evaluated in eutopic and ectopic endometrium of 30 patients with endometriosis (endometriosis group), and in eutopic endometrium of 22 patients without endometriosis (control group). The effect of BTG1 downregulation on cellular migration, proliferation, and apoptosis was evaluated using transfection of primarily cultured human endometrial stromal cells (HESCs) with <i>BTG1</i> siRNA. <i>BTG1</i> mRNA expression level of eutopic and ectopic endometrium of endometriosis group were significantly lower than that of the eutopic endometrium of the control group. Migration and wound healing assays revealed that BTG1 downregulation resulted in a significant increase in migration potential of HESCs, characterized by increased expression of matrix metalloproteinase 2 (MMP2) and MMP9. Downregulation of BTG1 in HESCs significantly reduced Caspase 3 expression, indicating a decrease in apoptotic potential. In conclusion, our data suggest that downregulation of BTG1 plays an important role in the pathogenesis of endometriosis. |
topic |
BTG1 endometriosis human endometrial stromal cells |
url |
https://www.mdpi.com/1422-0067/20/13/3372 |
work_keys_str_mv |
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