Carboxyl ester lipase
Carboxyl ester lipase (C33347), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of...
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doaj-9a06815c5ba548a79bc04dc7449188d02021-04-27T04:42:49ZengElsevierJournal of Lipid Research0022-22752002-12-01431220172030Carboxyl ester lipaseDavid Y. Hui0Philip N. Howles1Department of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OHDepartment of Pathology and Laboratory Medicine, University of Cincinnati College of Medicine, Cincinnati, OHCarboxyl ester lipase (C33347), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally located within the enzyme structure and is partially covered by a surface loop. The carboxyl terminus of the protein regulates enzymatic activity by forming hydrogen bonds with the surface loop to partially shield the active site. Bile salt binding to the loop domain frees the active site for accessibility by water-insoluble substrates. CEL is synthesized primarily in the pancreas and lactating mammary gland, but the enzyme is also expressed in liver, macrophages, and in the vessel wall. In the gastrointestinal tract, CEL serves as a compensatory protein to other lipolytic enzymes for complete digestion and absorption of lipid nutrients. Importantly, CEL also participates in chylomicron assembly and secretion, in a mechanism mediated through its ceramide hydrolytic activity. Cell culture studies suggest a role for CEL in lipoprotein metabolism and oxidized LDL-induced atherosclerosis.Thus, this enzyme, which has a wide substrate reactivity and diffuse anatomic distribution, may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis.http://www.sciencedirect.com/science/article/pii/S0022227520327279lipid absorptioncholesterol absorptionatherosclerosischylomicron assemblyreverse cholesterol transport |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
David Y. Hui Philip N. Howles |
spellingShingle |
David Y. Hui Philip N. Howles Carboxyl ester lipase Journal of Lipid Research lipid absorption cholesterol absorption atherosclerosis chylomicron assembly reverse cholesterol transport |
author_facet |
David Y. Hui Philip N. Howles |
author_sort |
David Y. Hui |
title |
Carboxyl ester lipase |
title_short |
Carboxyl ester lipase |
title_full |
Carboxyl ester lipase |
title_fullStr |
Carboxyl ester lipase |
title_full_unstemmed |
Carboxyl ester lipase |
title_sort |
carboxyl ester lipase |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
2002-12-01 |
description |
Carboxyl ester lipase (C33347), previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. The active site catalytic triad of serine-histidine-aspartate is centrally located within the enzyme structure and is partially covered by a surface loop. The carboxyl terminus of the protein regulates enzymatic activity by forming hydrogen bonds with the surface loop to partially shield the active site. Bile salt binding to the loop domain frees the active site for accessibility by water-insoluble substrates. CEL is synthesized primarily in the pancreas and lactating mammary gland, but the enzyme is also expressed in liver, macrophages, and in the vessel wall. In the gastrointestinal tract, CEL serves as a compensatory protein to other lipolytic enzymes for complete digestion and absorption of lipid nutrients. Importantly, CEL also participates in chylomicron assembly and secretion, in a mechanism mediated through its ceramide hydrolytic activity. Cell culture studies suggest a role for CEL in lipoprotein metabolism and oxidized LDL-induced atherosclerosis.Thus, this enzyme, which has a wide substrate reactivity and diffuse anatomic distribution, may have multiple functions in lipid and lipoprotein metabolism, and atherosclerosis. |
topic |
lipid absorption cholesterol absorption atherosclerosis chylomicron assembly reverse cholesterol transport |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520327279 |
work_keys_str_mv |
AT davidyhui carboxylesterlipase AT philipnhowles carboxylesterlipase |
_version_ |
1721506707676332032 |