Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line
Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants in the Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential. The present research aimed to inve...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR
2020-10-01
|
Series: | Iranian Journal of Chemistry & Chemical Engineering |
Subjects: | |
Online Access: | http://www.ijcce.ac.ir/article_36075_cda64ab0bbca235273d9aa420ba0972b.pdf |
id |
doaj-9a0bbcbd1c8b4e8a836528b72b494d02 |
---|---|
record_format |
Article |
spelling |
doaj-9a0bbcbd1c8b4e8a836528b72b494d022021-01-23T19:52:42ZengIranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECRIranian Journal of Chemistry & Chemical Engineering 1021-99861021-99862020-10-0139514515510.30492/ijcce.2019.3607536075Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell LineMaryam Azarian0Amir Amani1Mohammad Ali Faramarzi2Akram Eidi3Adeleh Divsalar4Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRANNatural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, I.R. IRANDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, I.R. IRANDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRANDepartment of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University Tehran, I.R. IRANNoscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants in the Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential. The present research aimed to investigate the induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspension of noscapine. Culture of HepG2 cells as spheroids was treated with different concentrations of noscapine for 24 h on Day 11. Afterward, viability assay and alkaline comet assay methods were applied to examine the viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween 40 on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline.http://www.ijcce.ac.ir/article_36075_cda64ab0bbca235273d9aa420ba0972b.pdfgenotoxicitynoscapinenanosuspensionmicrofluidic reactorshepg2 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maryam Azarian Amir Amani Mohammad Ali Faramarzi Akram Eidi Adeleh Divsalar |
spellingShingle |
Maryam Azarian Amir Amani Mohammad Ali Faramarzi Akram Eidi Adeleh Divsalar Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line Iranian Journal of Chemistry & Chemical Engineering genotoxicity noscapine nanosuspension microfluidic reactors hepg2 |
author_facet |
Maryam Azarian Amir Amani Mohammad Ali Faramarzi Akram Eidi Adeleh Divsalar |
author_sort |
Maryam Azarian |
title |
Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line |
title_short |
Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line |
title_full |
Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line |
title_fullStr |
Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line |
title_full_unstemmed |
Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line |
title_sort |
genotoxicity of noscapine nanosuspension prepared by microfluidic reactors on hepg2 cell line |
publisher |
Iranian Institute of Research and Development in Chemical Industries (IRDCI)-ACECR |
series |
Iranian Journal of Chemistry & Chemical Engineering |
issn |
1021-9986 1021-9986 |
publishDate |
2020-10-01 |
description |
Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants in the Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential. The present research aimed to investigate the induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspension of noscapine. Culture of HepG2 cells as spheroids was treated with different concentrations of noscapine for 24 h on Day 11. Afterward, viability assay and alkaline comet assay methods were applied to examine the viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween 40 on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline. |
topic |
genotoxicity noscapine nanosuspension microfluidic reactors hepg2 |
url |
http://www.ijcce.ac.ir/article_36075_cda64ab0bbca235273d9aa420ba0972b.pdf |
work_keys_str_mv |
AT maryamazarian genotoxicityofnoscapinenanosuspensionpreparedbymicrofluidicreactorsonhepg2cellline AT amiramani genotoxicityofnoscapinenanosuspensionpreparedbymicrofluidicreactorsonhepg2cellline AT mohammadalifaramarzi genotoxicityofnoscapinenanosuspensionpreparedbymicrofluidicreactorsonhepg2cellline AT akrameidi genotoxicityofnoscapinenanosuspensionpreparedbymicrofluidicreactorsonhepg2cellline AT adelehdivsalar genotoxicityofnoscapinenanosuspensionpreparedbymicrofluidicreactorsonhepg2cellline |
_version_ |
1724326993115217920 |