Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System.
The objective of this study was to evaluate the effects of partially replacing dry ground corn with glycerin on ruminal fermentation using a dual-flow continuous culture system. Six fermenters (1,223 ± 21 ml) were used in a replicated 3x3 Latin square arrangement with three periods of 10 d each, wit...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2015-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4657883?pdf=render |
id |
doaj-9a1c8163ee114511b8e525ffdc4bad83 |
---|---|
record_format |
Article |
spelling |
doaj-9a1c8163ee114511b8e525ffdc4bad832020-11-25T02:50:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014320110.1371/journal.pone.0143201Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System.Pedro Del Bianco BenedetiLorrayny Galoro da SilvaEduardo Marostegan de PaulaTeshome ShenkoruMarcos Inácio MarcondesHugo Fernando MonteiroBrad AmoratiYenling YehSimon Roger PoulsonAntonio Pinheiro FaciolaThe objective of this study was to evaluate the effects of partially replacing dry ground corn with glycerin on ruminal fermentation using a dual-flow continuous culture system. Six fermenters (1,223 ± 21 ml) were used in a replicated 3x3 Latin square arrangement with three periods of 10 d each, with 7 d for diet adaptation and 3 d for sample collections. All diets contained 75% concentrate and three dietary glycerin levels (0, 15, and 30% on dry matter basis), totaling six replicates per treatment. Fermenters were fed 72 g of dry matter/d equally divided in two meals/d, at 0800 and 2000 h. Solid and liquid dilution rates were adjusted daily to 5.5 and 11%/h, respectively. On d 8, 9, and 10, samples of 500 ml of solid and liquid digesta effluent were mixed, homogenized, and stored at -20°C. Subsamples of 10 ml were collected and preserved with 0.2 mL of a 50% H2SO4 solution for later determination of NH3-N and volatile fatty acids. Microbial biomass was isolated from fermenters for chemical analysis at the end of each experimental period. Data were analyzed using the MIXED procedure in SAS with α = 0.05. Glycerin levels did not affect apparent digestibility of DM (PLin. = 0.13; PQuad. = 0.40), OM (PLin. = 0.72; PQuad. = 0.15), NDF (PLin. = 0.38; PQuad. = 0.50) and ADF (PLin. = 0.91; PQuad. = 0.18). Also, glycerin inclusion did not affect true digestibility of DM (PLin. = 0.35; PQuad. = 0.48), and OM (PLin. = 0.08; PQuad. = 0.19). Concentrations of propionate (P < 0.01) and total volatile fatty acids (P < 0.01) increased linearly and concentrations of acetate (P < 0.01), butyrate (P = 0.01), iso-valerate (P < 0.01), and total branched-chain volatile fatty acids, as well as the acetate: propionate ratio (P < 0.01) decreased with glycerin inclusion. Linear increases on NH3-N concentration in digesta effluent (P < 0.01) and on NH3-N flow (P < 0.01) were observed due to glycerin inclusion in the diets. Crude protein digestibility (P = 0.04) and microbial N flow (P = 0.04) were greater in the control treatment compared with the other treatments and responded quadratically with glycerin inclusion. Furthermore, the inclusion of glycerin linearly decreased (P = 0.02) non-ammonia N flow. Glycerin levels did not affect the flows of total N (PLin. = 0.79; PQuad. = 0.35), and dietary N (PLin. = 0.99; PQuad. = 0.07), as well as microbial efficiency (PLin. = 0.09; PQuad. = 0.07). These results suggest that partially replacing dry ground corn with glycerin may change ruminal fermentation, by increasing total volatile fatty acids, and propionate concentration without affecting microbial efficiency, which may improve glucogenic potential of beef cattle diets.http://europepmc.org/articles/PMC4657883?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pedro Del Bianco Benedeti Lorrayny Galoro da Silva Eduardo Marostegan de Paula Teshome Shenkoru Marcos Inácio Marcondes Hugo Fernando Monteiro Brad Amorati Yenling Yeh Simon Roger Poulson Antonio Pinheiro Faciola |
spellingShingle |
Pedro Del Bianco Benedeti Lorrayny Galoro da Silva Eduardo Marostegan de Paula Teshome Shenkoru Marcos Inácio Marcondes Hugo Fernando Monteiro Brad Amorati Yenling Yeh Simon Roger Poulson Antonio Pinheiro Faciola Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. PLoS ONE |
author_facet |
Pedro Del Bianco Benedeti Lorrayny Galoro da Silva Eduardo Marostegan de Paula Teshome Shenkoru Marcos Inácio Marcondes Hugo Fernando Monteiro Brad Amorati Yenling Yeh Simon Roger Poulson Antonio Pinheiro Faciola |
author_sort |
Pedro Del Bianco Benedeti |
title |
Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. |
title_short |
Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. |
title_full |
Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. |
title_fullStr |
Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. |
title_full_unstemmed |
Effects of Partial Replacement of Corn with Glycerin on Ruminal Fermentation in a Dual-Flow Continuous Culture System. |
title_sort |
effects of partial replacement of corn with glycerin on ruminal fermentation in a dual-flow continuous culture system. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2015-01-01 |
description |
The objective of this study was to evaluate the effects of partially replacing dry ground corn with glycerin on ruminal fermentation using a dual-flow continuous culture system. Six fermenters (1,223 ± 21 ml) were used in a replicated 3x3 Latin square arrangement with three periods of 10 d each, with 7 d for diet adaptation and 3 d for sample collections. All diets contained 75% concentrate and three dietary glycerin levels (0, 15, and 30% on dry matter basis), totaling six replicates per treatment. Fermenters were fed 72 g of dry matter/d equally divided in two meals/d, at 0800 and 2000 h. Solid and liquid dilution rates were adjusted daily to 5.5 and 11%/h, respectively. On d 8, 9, and 10, samples of 500 ml of solid and liquid digesta effluent were mixed, homogenized, and stored at -20°C. Subsamples of 10 ml were collected and preserved with 0.2 mL of a 50% H2SO4 solution for later determination of NH3-N and volatile fatty acids. Microbial biomass was isolated from fermenters for chemical analysis at the end of each experimental period. Data were analyzed using the MIXED procedure in SAS with α = 0.05. Glycerin levels did not affect apparent digestibility of DM (PLin. = 0.13; PQuad. = 0.40), OM (PLin. = 0.72; PQuad. = 0.15), NDF (PLin. = 0.38; PQuad. = 0.50) and ADF (PLin. = 0.91; PQuad. = 0.18). Also, glycerin inclusion did not affect true digestibility of DM (PLin. = 0.35; PQuad. = 0.48), and OM (PLin. = 0.08; PQuad. = 0.19). Concentrations of propionate (P < 0.01) and total volatile fatty acids (P < 0.01) increased linearly and concentrations of acetate (P < 0.01), butyrate (P = 0.01), iso-valerate (P < 0.01), and total branched-chain volatile fatty acids, as well as the acetate: propionate ratio (P < 0.01) decreased with glycerin inclusion. Linear increases on NH3-N concentration in digesta effluent (P < 0.01) and on NH3-N flow (P < 0.01) were observed due to glycerin inclusion in the diets. Crude protein digestibility (P = 0.04) and microbial N flow (P = 0.04) were greater in the control treatment compared with the other treatments and responded quadratically with glycerin inclusion. Furthermore, the inclusion of glycerin linearly decreased (P = 0.02) non-ammonia N flow. Glycerin levels did not affect the flows of total N (PLin. = 0.79; PQuad. = 0.35), and dietary N (PLin. = 0.99; PQuad. = 0.07), as well as microbial efficiency (PLin. = 0.09; PQuad. = 0.07). These results suggest that partially replacing dry ground corn with glycerin may change ruminal fermentation, by increasing total volatile fatty acids, and propionate concentration without affecting microbial efficiency, which may improve glucogenic potential of beef cattle diets. |
url |
http://europepmc.org/articles/PMC4657883?pdf=render |
work_keys_str_mv |
AT pedrodelbiancobenedeti effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT lorraynygalorodasilva effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT eduardomarostegandepaula effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT teshomeshenkoru effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT marcosinaciomarcondes effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT hugofernandomonteiro effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT bradamorati effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT yenlingyeh effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT simonrogerpoulson effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem AT antoniopinheirofaciola effectsofpartialreplacementofcornwithglycerinonruminalfermentationinadualflowcontinuousculturesystem |
_version_ |
1724736657037459456 |