Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome.
As much of the aberrant neural development in Down syndrome (DS) occurs postnatally, an early opportunity exists to intervene and influence life-long cognitive development. Recent success using neural progenitor cells (NPC) in models of adult neurodegeneration indicate such therapy may be a viable o...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2012-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3338504?pdf=render |
id |
doaj-9a3b1f550e1740a6be9e8f666952b25e |
---|---|
record_format |
Article |
spelling |
doaj-9a3b1f550e1740a6be9e8f666952b25e2020-11-25T00:40:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0174e3608210.1371/journal.pone.0036082Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome.Angela L RachubinskiShannon K CrowleyJohn R SladekKenneth N MacleanKimberly B BjugstadAs much of the aberrant neural development in Down syndrome (DS) occurs postnatally, an early opportunity exists to intervene and influence life-long cognitive development. Recent success using neural progenitor cells (NPC) in models of adult neurodegeneration indicate such therapy may be a viable option in diseases such as DS. Murine NPC (mNPC, C17.2 cell line) or saline were implanted bilaterally into the dorsal hippocampus of postnatal day 2 (PND 2) Ts65Dn pups to explore the feasibility of early postnatal treatment in this mouse model of DS. Disomic littermates provided karyotype controls for trisomic pups. Pups were monitored for developmental milestone achievement, and then underwent adult behavior testing at 14 weeks of age. We found that implanted mNPC survived into adulthood and migrated beyond the implant site in both karyotypes. The implantation of mNPC resulted in a significant increase in the density of dentate granule cells. However, mNPC implantation did not elicit cognitive changes in trisomic mice either neonatally or in adulthood. To the best of our knowledge, these results constitute the first assessment of mNPC as an early intervention on cognitive ability in a DS model.http://europepmc.org/articles/PMC3338504?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Angela L Rachubinski Shannon K Crowley John R Sladek Kenneth N Maclean Kimberly B Bjugstad |
spellingShingle |
Angela L Rachubinski Shannon K Crowley John R Sladek Kenneth N Maclean Kimberly B Bjugstad Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. PLoS ONE |
author_facet |
Angela L Rachubinski Shannon K Crowley John R Sladek Kenneth N Maclean Kimberly B Bjugstad |
author_sort |
Angela L Rachubinski |
title |
Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. |
title_short |
Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. |
title_full |
Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. |
title_fullStr |
Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. |
title_full_unstemmed |
Effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the Ts65Dn model of Down syndrome. |
title_sort |
effects of neonatal neural progenitor cell implantation on adult neuroanatomy and cognition in the ts65dn model of down syndrome. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
As much of the aberrant neural development in Down syndrome (DS) occurs postnatally, an early opportunity exists to intervene and influence life-long cognitive development. Recent success using neural progenitor cells (NPC) in models of adult neurodegeneration indicate such therapy may be a viable option in diseases such as DS. Murine NPC (mNPC, C17.2 cell line) or saline were implanted bilaterally into the dorsal hippocampus of postnatal day 2 (PND 2) Ts65Dn pups to explore the feasibility of early postnatal treatment in this mouse model of DS. Disomic littermates provided karyotype controls for trisomic pups. Pups were monitored for developmental milestone achievement, and then underwent adult behavior testing at 14 weeks of age. We found that implanted mNPC survived into adulthood and migrated beyond the implant site in both karyotypes. The implantation of mNPC resulted in a significant increase in the density of dentate granule cells. However, mNPC implantation did not elicit cognitive changes in trisomic mice either neonatally or in adulthood. To the best of our knowledge, these results constitute the first assessment of mNPC as an early intervention on cognitive ability in a DS model. |
url |
http://europepmc.org/articles/PMC3338504?pdf=render |
work_keys_str_mv |
AT angelalrachubinski effectsofneonatalneuralprogenitorcellimplantationonadultneuroanatomyandcognitioninthets65dnmodelofdownsyndrome AT shannonkcrowley effectsofneonatalneuralprogenitorcellimplantationonadultneuroanatomyandcognitioninthets65dnmodelofdownsyndrome AT johnrsladek effectsofneonatalneuralprogenitorcellimplantationonadultneuroanatomyandcognitioninthets65dnmodelofdownsyndrome AT kennethnmaclean effectsofneonatalneuralprogenitorcellimplantationonadultneuroanatomyandcognitioninthets65dnmodelofdownsyndrome AT kimberlybbjugstad effectsofneonatalneuralprogenitorcellimplantationonadultneuroanatomyandcognitioninthets65dnmodelofdownsyndrome |
_version_ |
1725288411521089536 |