Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1
Background/Aims: Sonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibroge...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Physiol Biochem Press GmbH & Co KG
2016-11-01
|
Series: | Cellular Physiology and Biochemistry |
Subjects: | |
Online Access: | http://www.karger.com/Article/FullText/452571 |
id |
doaj-9a3c81d4a3a64ee5ac72795f70d11cb6 |
---|---|
record_format |
Article |
spelling |
doaj-9a3c81d4a3a64ee5ac72795f70d11cb62020-11-25T01:00:16ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-11-01403-457958810.1159/000452571452571Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1Yuanyuan GuoZengxiang DongYuanqi ShiWei WangLu WangJing SunXin SunZhen TianJianting YaoZhitao LiJiali ChengYe TianBackground/Aims: Sonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibrogenesis in rat cardiac fibroblasts. Methods: The primary cardiac fibroblasts were isolated from rats, and induced to fibrogenesis with TGF-β1. With this in vitro model, we tested the preventive effects of SDT on fibrogenesis and further the underlying mechanism. Results: TGF-β1 stimulation up-regulated α-SMA and COLI/III protein levels in cardiac fibroblasts, and enhanced the progression of cells from the G0/G1 phase to the S phase. SDT inhibited the TGF-β1 mediated cell proliferation and decreased the levels of α-SMA and COLI/III by activating AKT/GSK3β pathway and blocking TGF-β1/SMAD3 signaling. Conclusion: Our studies demonstrate an antifibrotic effect of SDT in rat cardiac fibroblasts, suggesting that SDT may intervene cardiac fibrogenesis by regulating myocardial fibrotic remodeling.http://www.karger.com/Article/FullText/452571SDTCardiac fibroblastTGF-β1GSK3βAKT |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuanyuan Guo Zengxiang Dong Yuanqi Shi Wei Wang Lu Wang Jing Sun Xin Sun Zhen Tian Jianting Yao Zhitao Li Jiali Cheng Ye Tian |
spellingShingle |
Yuanyuan Guo Zengxiang Dong Yuanqi Shi Wei Wang Lu Wang Jing Sun Xin Sun Zhen Tian Jianting Yao Zhitao Li Jiali Cheng Ye Tian Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 Cellular Physiology and Biochemistry SDT Cardiac fibroblast TGF-β1 GSK3β AKT |
author_facet |
Yuanyuan Guo Zengxiang Dong Yuanqi Shi Wei Wang Lu Wang Jing Sun Xin Sun Zhen Tian Jianting Yao Zhitao Li Jiali Cheng Ye Tian |
author_sort |
Yuanyuan Guo |
title |
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 |
title_short |
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 |
title_full |
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 |
title_fullStr |
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 |
title_full_unstemmed |
Sonodynamic Therapy Inhibits Fibrogenesis in Rat Cardiac Fibroblasts Induced by TGF-β1 |
title_sort |
sonodynamic therapy inhibits fibrogenesis in rat cardiac fibroblasts induced by tgf-β1 |
publisher |
Cell Physiol Biochem Press GmbH & Co KG |
series |
Cellular Physiology and Biochemistry |
issn |
1015-8987 1421-9778 |
publishDate |
2016-11-01 |
description |
Background/Aims: Sonodynamic therapy (SDT) is a localized ultrasound-activated therapy for atherosclerosis when combined with a sonosensitizer, 5-aminolevulinic acid (ALA), but whether it can prevent cardiac fibrosis has not been studied. In the present study, we evaluated the effects SDT on fibrogenesis in rat cardiac fibroblasts. Methods: The primary cardiac fibroblasts were isolated from rats, and induced to fibrogenesis with TGF-β1. With this in vitro model, we tested the preventive effects of SDT on fibrogenesis and further the underlying mechanism. Results: TGF-β1 stimulation up-regulated α-SMA and COLI/III protein levels in cardiac fibroblasts, and enhanced the progression of cells from the G0/G1 phase to the S phase. SDT inhibited the TGF-β1 mediated cell proliferation and decreased the levels of α-SMA and COLI/III by activating AKT/GSK3β pathway and blocking TGF-β1/SMAD3 signaling. Conclusion: Our studies demonstrate an antifibrotic effect of SDT in rat cardiac fibroblasts, suggesting that SDT may intervene cardiac fibrogenesis by regulating myocardial fibrotic remodeling. |
topic |
SDT Cardiac fibroblast TGF-β1 GSK3β AKT |
url |
http://www.karger.com/Article/FullText/452571 |
work_keys_str_mv |
AT yuanyuanguo sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT zengxiangdong sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT yuanqishi sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT weiwang sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT luwang sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT jingsun sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT xinsun sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT zhentian sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT jiantingyao sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT zhitaoli sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT jialicheng sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 AT yetian sonodynamictherapyinhibitsfibrogenesisinratcardiacfibroblastsinducedbytgfb1 |
_version_ |
1725214401003257856 |