Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein
The phenotypes of calbindin-D9k- (CaBP-9k-) knockout (KO), calbindin-D28k- (CaBP-28k-) KO, and CaBP-9k/28k-KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we investigated the expression of cellular prion protein (...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
2018-01-01
|
Series: | Oxidative Medicine and Cellular Longevity |
Online Access: | http://dx.doi.org/10.1155/2018/4670210 |
id |
doaj-9a3cf8d9c88d42deb9a5121a441ae393 |
---|---|
record_format |
Article |
spelling |
doaj-9a3cf8d9c88d42deb9a5121a441ae3932020-11-24T23:12:49ZengHindawi LimitedOxidative Medicine and Cellular Longevity1942-09001942-09942018-01-01201810.1155/2018/46702104670210Calbindin-D28k in the Brain Influences the Expression of Cellular Prion ProteinYeong-Min Yoo0Eui-Bae Jeung1Institute of Forest Science, Department of Forest Environment Protection, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon, 24341 Gangwon-do, Republic of KoreaLaboratory of Veterinary Biochemistry and Molecular Biology, College of Veterinary Medicine, Chungbuk National University, Cheongju, 28644 Chungbuk, Republic of KoreaThe phenotypes of calbindin-D9k- (CaBP-9k-) knockout (KO), calbindin-D28k- (CaBP-28k-) KO, and CaBP-9k/28k-KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we investigated the expression of cellular prion protein (PrPC) in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice. PrPC expression was significantly upregulated in the brain of all three strains. Levels of phospho-Akt (Ser473) and phospho-Bad (Ser136) were significantly elevated, but those of phospho-ERK and phospho-Bad (Ser155 and 112) were significantly reduced in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice. The expressions of the Bcl-2, p53, Bax, Cu/Zn-SOD, and Mn-SOD proteins were decreased in the brains of all KO mice. Expression of the endoplasmic reticulum marker protein BiP/GRP78 was decreased, and that of the CHOP protein was increased in the brains of those KO mice. To identify the roles of CaBP-28k, we transfected PC12 cells with siRNA for CaBP-28k and found increased expression of the PrPC protein compared to the levels in control cells. These results suggest that CaBP-28k expression may regulate PrPC protein expression and these mice may be vulnerable to the influence of prion disease.http://dx.doi.org/10.1155/2018/4670210 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yeong-Min Yoo Eui-Bae Jeung |
spellingShingle |
Yeong-Min Yoo Eui-Bae Jeung Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein Oxidative Medicine and Cellular Longevity |
author_facet |
Yeong-Min Yoo Eui-Bae Jeung |
author_sort |
Yeong-Min Yoo |
title |
Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein |
title_short |
Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein |
title_full |
Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein |
title_fullStr |
Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein |
title_full_unstemmed |
Calbindin-D28k in the Brain Influences the Expression of Cellular Prion Protein |
title_sort |
calbindin-d28k in the brain influences the expression of cellular prion protein |
publisher |
Hindawi Limited |
series |
Oxidative Medicine and Cellular Longevity |
issn |
1942-0900 1942-0994 |
publishDate |
2018-01-01 |
description |
The phenotypes of calbindin-D9k- (CaBP-9k-) knockout (KO), calbindin-D28k- (CaBP-28k-) KO, and CaBP-9k/28k-KO mice are similar to those of wild-type (WT) mice due to the compensatory action of other calcium transport proteins. In this study, we investigated the expression of cellular prion protein (PrPC) in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice. PrPC expression was significantly upregulated in the brain of all three strains. Levels of phospho-Akt (Ser473) and phospho-Bad (Ser136) were significantly elevated, but those of phospho-ERK and phospho-Bad (Ser155 and 112) were significantly reduced in the brains of CaBP-9k-, CaBP-28k-, and CaBP-9k/28k-KO mice. The expressions of the Bcl-2, p53, Bax, Cu/Zn-SOD, and Mn-SOD proteins were decreased in the brains of all KO mice. Expression of the endoplasmic reticulum marker protein BiP/GRP78 was decreased, and that of the CHOP protein was increased in the brains of those KO mice. To identify the roles of CaBP-28k, we transfected PC12 cells with siRNA for CaBP-28k and found increased expression of the PrPC protein compared to the levels in control cells. These results suggest that CaBP-28k expression may regulate PrPC protein expression and these mice may be vulnerable to the influence of prion disease. |
url |
http://dx.doi.org/10.1155/2018/4670210 |
work_keys_str_mv |
AT yeongminyoo calbindind28kinthebraininfluencestheexpressionofcellularprionprotein AT euibaejeung calbindind28kinthebraininfluencestheexpressionofcellularprionprotein |
_version_ |
1725600660226834432 |