Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy
Understanding factors that impact CAR T cell expansion in the clinic is crucial to improving its therapeutic success. Here the authors document heterogeneity in the clonal dynamics of CAR-T cells by tracking individual clones using the endogenous TCR and integration sites and provide further insight...
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| Language: | English |
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Nature Publishing Group
2020-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-13880-1 |
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doaj-9a5dfb14385d4c6ba59269c0303b61902021-05-11T09:11:16ZengNature Publishing GroupNature Communications2041-17232020-01-0111111310.1038/s41467-019-13880-1Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapyAlyssa Sheih0Valentin Voillet1Laïla-Aïcha Hanafi2Hannah A. DeBerg3Masanao Yajima4Reed Hawkins5Vivian Gersuk6Stanley R. Riddell7David G. Maloney8Martin E. Wohlfahrt9Dnyanada Pande10Mark R. Enstrom11Hans-Peter Kiem12Jennifer E. Adair13Raphaël Gottardo14Peter S. Linsley15Cameron J. Turtle16Clinical Research Division, Fred Hutchinson Cancer Research CenterVaccine and Infectious Disease Division and Public Health Sciences Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterBenaroya Research Institute at Virginia MasonDepartment of Mathematics and Statistics, Boston UniversityClinical Research Division, Fred Hutchinson Cancer Research CenterBenaroya Research Institute at Virginia MasonClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterClinical Research Division, Fred Hutchinson Cancer Research CenterVaccine and Infectious Disease Division and Public Health Sciences Division, Fred Hutchinson Cancer Research CenterBenaroya Research Institute at Virginia MasonClinical Research Division, Fred Hutchinson Cancer Research CenterUnderstanding factors that impact CAR T cell expansion in the clinic is crucial to improving its therapeutic success. Here the authors document heterogeneity in the clonal dynamics of CAR-T cells by tracking individual clones using the endogenous TCR and integration sites and provide further insights into the role of transcriptional states in clonal kinetics.https://doi.org/10.1038/s41467-019-13880-1 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Alyssa Sheih Valentin Voillet Laïla-Aïcha Hanafi Hannah A. DeBerg Masanao Yajima Reed Hawkins Vivian Gersuk Stanley R. Riddell David G. Maloney Martin E. Wohlfahrt Dnyanada Pande Mark R. Enstrom Hans-Peter Kiem Jennifer E. Adair Raphaël Gottardo Peter S. Linsley Cameron J. Turtle |
| spellingShingle |
Alyssa Sheih Valentin Voillet Laïla-Aïcha Hanafi Hannah A. DeBerg Masanao Yajima Reed Hawkins Vivian Gersuk Stanley R. Riddell David G. Maloney Martin E. Wohlfahrt Dnyanada Pande Mark R. Enstrom Hans-Peter Kiem Jennifer E. Adair Raphaël Gottardo Peter S. Linsley Cameron J. Turtle Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy Nature Communications |
| author_facet |
Alyssa Sheih Valentin Voillet Laïla-Aïcha Hanafi Hannah A. DeBerg Masanao Yajima Reed Hawkins Vivian Gersuk Stanley R. Riddell David G. Maloney Martin E. Wohlfahrt Dnyanada Pande Mark R. Enstrom Hans-Peter Kiem Jennifer E. Adair Raphaël Gottardo Peter S. Linsley Cameron J. Turtle |
| author_sort |
Alyssa Sheih |
| title |
Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy |
| title_short |
Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy |
| title_full |
Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy |
| title_fullStr |
Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy |
| title_full_unstemmed |
Clonal kinetics and single-cell transcriptional profiling of CAR-T cells in patients undergoing CD19 CAR-T immunotherapy |
| title_sort |
clonal kinetics and single-cell transcriptional profiling of car-t cells in patients undergoing cd19 car-t immunotherapy |
| publisher |
Nature Publishing Group |
| series |
Nature Communications |
| issn |
2041-1723 |
| publishDate |
2020-01-01 |
| description |
Understanding factors that impact CAR T cell expansion in the clinic is crucial to improving its therapeutic success. Here the authors document heterogeneity in the clonal dynamics of CAR-T cells by tracking individual clones using the endogenous TCR and integration sites and provide further insights into the role of transcriptional states in clonal kinetics. |
| url |
https://doi.org/10.1038/s41467-019-13880-1 |
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