The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice
Abstract Background This study was aimed to determination the tumor inhibitory effect and explore the potential mechanisms of Lagopsis supine ethanol extract (Ls) on colorectal cancer. Methods The cell growth inhibition experiment of Ls in colorectal cancer cell lines was determined by MTT method in...
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doaj-9a71e36e8e534934b51325b68198e2722020-11-25T02:37:34ZengBMCBMC Complementary and Alternative Medicine1472-68822019-07-0119111010.1186/s12906-019-2585-6The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude miceLijuan Wei0Zhaoyong Wang1Yang Xia2Baichun Liu3Department of Gastroenterology and Digestive Endoscopy Center, The Second Hospital of Jilin UniversityDepartment of Pathology, The Second Hospital of Jilin UniversityDepartment of Pathology, The Second Hospital of Jilin UniversityDepartment of Gastroenterology and Digestive Endoscopy Center, The Second Hospital of Jilin UniversityAbstract Background This study was aimed to determination the tumor inhibitory effect and explore the potential mechanisms of Lagopsis supine ethanol extract (Ls) on colorectal cancer. Methods The cell growth inhibition experiment of Ls in colorectal cancer cell lines was determined by MTT method in the time course of 24, 48 and 72 h in four gradient drug concentrations. The protein expression levels of pSTAT3, pJAK2, STAT3, JAK2, Bcl-2 and caspase 3 were measured by Western blot method. The mRNA levels of the downstream genes of STAT3 were detected through semi-quantitative RT PCR. Sixty Balb/c-nude mice were xenograft with HCT116 colorectal cancer cells through subcutaneously. The xenografts were divided into five groups: model group, positive group (capecitabine 300 mg/kg) and three dosages of Ls treated groups (75, 150 and 300 mg/kg). Tumor size and tumor weight were calculated for evaluation the anti-tumor effects. H & E staining and immunohistochemical analysis were used to determine the histopathological changes and the levels of pSTAT3 and pJAK2 in the tumor tissues. Results Ls exhibited a significant anti-proliferation effect in HCT116 and SW480 cells in vitro. The protein levels of pSTAT3, pJAK2 and Bcl-2, and the mRNA levels of Bcl-2 and Bak notably reduced with a dose-dependent manner. While the protein levels of caspase 3, and mRNA levels of Bax and caspase-3 remarkably increased in the gradient dosage of Ls in HCT116 cells. HCT116 in vivo xenografts experiment showed that the growth of the tumors significantly inhibited by Ls administration, which with no any significant body weight changes in each experiment group. The histopathology analysis displayed that Ls significantly reduced the inflammatory cells in tumor tissue. Furthermore, Ls also significantly down-regulate the protein levels of pSTAT3 and pJAK2 in the tumor tissues, compared with the model group. Conclusions This work shows that Ls inhibited the cell proliferation of colorectal cancer in vitro and significantly reduced the tumor growth in HCT116 xenografts in vivo, which is probably related with the JAK/STAT signal pathway.http://link.springer.com/article/10.1186/s12906-019-2585-6Lagopsis supine ethanol extractColorectal carcinomaJAK/STAT signal pathwayTumor xenografts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lijuan Wei Zhaoyong Wang Yang Xia Baichun Liu |
spellingShingle |
Lijuan Wei Zhaoyong Wang Yang Xia Baichun Liu The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice BMC Complementary and Alternative Medicine Lagopsis supine ethanol extract Colorectal carcinoma JAK/STAT signal pathway Tumor xenografts |
author_facet |
Lijuan Wei Zhaoyong Wang Yang Xia Baichun Liu |
author_sort |
Lijuan Wei |
title |
The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice |
title_short |
The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice |
title_full |
The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice |
title_fullStr |
The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice |
title_full_unstemmed |
The mechanism and tumor inhibitory study of Lagopsis supine ethanol extract on colorectal cancer in nude mice |
title_sort |
mechanism and tumor inhibitory study of lagopsis supine ethanol extract on colorectal cancer in nude mice |
publisher |
BMC |
series |
BMC Complementary and Alternative Medicine |
issn |
1472-6882 |
publishDate |
2019-07-01 |
description |
Abstract Background This study was aimed to determination the tumor inhibitory effect and explore the potential mechanisms of Lagopsis supine ethanol extract (Ls) on colorectal cancer. Methods The cell growth inhibition experiment of Ls in colorectal cancer cell lines was determined by MTT method in the time course of 24, 48 and 72 h in four gradient drug concentrations. The protein expression levels of pSTAT3, pJAK2, STAT3, JAK2, Bcl-2 and caspase 3 were measured by Western blot method. The mRNA levels of the downstream genes of STAT3 were detected through semi-quantitative RT PCR. Sixty Balb/c-nude mice were xenograft with HCT116 colorectal cancer cells through subcutaneously. The xenografts were divided into five groups: model group, positive group (capecitabine 300 mg/kg) and three dosages of Ls treated groups (75, 150 and 300 mg/kg). Tumor size and tumor weight were calculated for evaluation the anti-tumor effects. H & E staining and immunohistochemical analysis were used to determine the histopathological changes and the levels of pSTAT3 and pJAK2 in the tumor tissues. Results Ls exhibited a significant anti-proliferation effect in HCT116 and SW480 cells in vitro. The protein levels of pSTAT3, pJAK2 and Bcl-2, and the mRNA levels of Bcl-2 and Bak notably reduced with a dose-dependent manner. While the protein levels of caspase 3, and mRNA levels of Bax and caspase-3 remarkably increased in the gradient dosage of Ls in HCT116 cells. HCT116 in vivo xenografts experiment showed that the growth of the tumors significantly inhibited by Ls administration, which with no any significant body weight changes in each experiment group. The histopathology analysis displayed that Ls significantly reduced the inflammatory cells in tumor tissue. Furthermore, Ls also significantly down-regulate the protein levels of pSTAT3 and pJAK2 in the tumor tissues, compared with the model group. Conclusions This work shows that Ls inhibited the cell proliferation of colorectal cancer in vitro and significantly reduced the tumor growth in HCT116 xenografts in vivo, which is probably related with the JAK/STAT signal pathway. |
topic |
Lagopsis supine ethanol extract Colorectal carcinoma JAK/STAT signal pathway Tumor xenografts |
url |
http://link.springer.com/article/10.1186/s12906-019-2585-6 |
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