Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation
Many studies have sought to construct a substitute to partially replace irreparably damaged meniscus. Only the meniscus allograft has been used in clinical practice as a useful substitute, and there are concerns about its longevity and inherent limitations, including availability of donor tissue and...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2020-07-01
|
Series: | Frontiers in Bioengineering and Biotechnology |
Subjects: | |
Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00779/full |
id |
doaj-9a77acbb15e94d2eace2f4aa6676a8b1 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yong He Yong He Yunbin Chen Yunbin Chen Yunbin Chen Xinyu Wan Xinyu Wan Xinyu Wan Chenchen Zhao Chenchen Zhao Pengcheng Qiu Pengcheng Qiu Xianfeng Lin Xianfeng Lin Jianfeng Zhang Jianfeng Zhang Yue Huang Yue Huang |
spellingShingle |
Yong He Yong He Yunbin Chen Yunbin Chen Yunbin Chen Xinyu Wan Xinyu Wan Xinyu Wan Chenchen Zhao Chenchen Zhao Pengcheng Qiu Pengcheng Qiu Xianfeng Lin Xianfeng Lin Jianfeng Zhang Jianfeng Zhang Yue Huang Yue Huang Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation Frontiers in Bioengineering and Biotechnology meniscus extracellular matrix biomechanics region-specific recellularization decellularization |
author_facet |
Yong He Yong He Yunbin Chen Yunbin Chen Yunbin Chen Xinyu Wan Xinyu Wan Xinyu Wan Chenchen Zhao Chenchen Zhao Pengcheng Qiu Pengcheng Qiu Xianfeng Lin Xianfeng Lin Jianfeng Zhang Jianfeng Zhang Yue Huang Yue Huang |
author_sort |
Yong He |
title |
Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation |
title_short |
Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation |
title_full |
Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation |
title_fullStr |
Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation |
title_full_unstemmed |
Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus Transplantation |
title_sort |
preparation and characterization of an optimized meniscal extracellular matrix scaffold for meniscus transplantation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2020-07-01 |
description |
Many studies have sought to construct a substitute to partially replace irreparably damaged meniscus. Only the meniscus allograft has been used in clinical practice as a useful substitute, and there are concerns about its longevity and inherent limitations, including availability of donor tissue and possibility of disease transmission. To overcome these limitations, we developed an acellular xenograft from whole porcine meniscus. Samples were treated with 2% Triton X-100 for 10 days and 2% sodium dodecyl sulfate for 6 days. The DNA content of extracellular matrix (ECM) scaffolds was significantly decreased compared with that of normal porcine menisci (p < 0.001). Histological analysis confirmed the maintenance of ECM integrity and anisotropic architecture in the absence of nuclei. Biochemical and biomechanical assays of the scaffolds indicated the preservation of collagen (p = 0.806), glycosaminoglycan (p = 0.188), and biomechanical properties (elastic modulus and transition stress). The scaffolds possessed good biocompatibility and supported bone marrow mesenchymal stem cells (BMSCs) proliferation for 2 weeks in vitro, with excellent region-specific recellularization in vivo. The novel scaffold has potential value for application in recellularization and transplantation strategies. |
topic |
meniscus extracellular matrix biomechanics region-specific recellularization decellularization |
url |
https://www.frontiersin.org/article/10.3389/fbioe.2020.00779/full |
work_keys_str_mv |
AT yonghe preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yonghe preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yunbinchen preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yunbinchen preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yunbinchen preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT xinyuwan preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT xinyuwan preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT xinyuwan preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT chenchenzhao preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT chenchenzhao preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT pengchengqiu preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT pengchengqiu preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT xianfenglin preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT xianfenglin preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT jianfengzhang preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT jianfengzhang preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yuehuang preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation AT yuehuang preparationandcharacterizationofanoptimizedmeniscalextracellularmatrixscaffoldformeniscustransplantation |
_version_ |
1724498052368039936 |
spelling |
doaj-9a77acbb15e94d2eace2f4aa6676a8b12020-11-25T03:48:36ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-07-01810.3389/fbioe.2020.00779528848Preparation and Characterization of an Optimized Meniscal Extracellular Matrix Scaffold for Meniscus TransplantationYong He0Yong He1Yunbin Chen2Yunbin Chen3Yunbin Chen4Xinyu Wan5Xinyu Wan6Xinyu Wan7Chenchen Zhao8Chenchen Zhao9Pengcheng Qiu10Pengcheng Qiu11Xianfeng Lin12Xianfeng Lin13Jianfeng Zhang14Jianfeng Zhang15Yue Huang16Yue Huang17Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Neurology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaFirst Clinical College, Wenzhou Medical University, Wenzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaDepartment of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University, Hangzhou, ChinaKey Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, ChinaMany studies have sought to construct a substitute to partially replace irreparably damaged meniscus. Only the meniscus allograft has been used in clinical practice as a useful substitute, and there are concerns about its longevity and inherent limitations, including availability of donor tissue and possibility of disease transmission. To overcome these limitations, we developed an acellular xenograft from whole porcine meniscus. Samples were treated with 2% Triton X-100 for 10 days and 2% sodium dodecyl sulfate for 6 days. The DNA content of extracellular matrix (ECM) scaffolds was significantly decreased compared with that of normal porcine menisci (p < 0.001). Histological analysis confirmed the maintenance of ECM integrity and anisotropic architecture in the absence of nuclei. Biochemical and biomechanical assays of the scaffolds indicated the preservation of collagen (p = 0.806), glycosaminoglycan (p = 0.188), and biomechanical properties (elastic modulus and transition stress). The scaffolds possessed good biocompatibility and supported bone marrow mesenchymal stem cells (BMSCs) proliferation for 2 weeks in vitro, with excellent region-specific recellularization in vivo. The novel scaffold has potential value for application in recellularization and transplantation strategies.https://www.frontiersin.org/article/10.3389/fbioe.2020.00779/fullmeniscusextracellular matrixbiomechanicsregion-specific recellularizationdecellularization |