Influence of the stationary and mobile phase composition in ideal chromatographic systems on the log k values in column chromatography. I. ODS/methanol-water system

• Main Authors: Janjić T.J., Vučković G., Ćelap M.В.
• Format: Article
• Language: English
• Published: Serbian Chemical Society 2000-01-01
• Series: Journal of the Serbian Chemical Society
• Subjects: c18 liquid chromatography; stationary phase composition; mobile phase composition; capacity factors; pmn parameter; log k pair linearity rule; proportionality rule; rpp scale
• Online Access: http://www.doiserbia.nb.rs/img/doi/0352-5139/2000/0352-51390010725J.pdf

On the basis of the literature data it was established that in the case of ODS column chromatography with methanol as modifier the value log xs/x1 (where xs and X1 denote methanol mole fractions in the stationary and the mobile phase, respectively) is a linear function of the corresponding Bosch’s PmN parameter as well as of the log k value of the investigated substances. It was also found that the phase equilibrium in the system ODS/methanol-water is in accordance with the Everett’s equation for ideal systems (K=10.88). Finally, a linear relationship between the log xs/x1 values and the volume fraction of methanol (φ) was found in the range of 0.5-1.0, which corresponds to the linear part of the function log k = f(φ), established experimentally by other authors.