Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy
Japanese cedar pollinosis is a type I allergic disease and has already become a major public health problem in Japan. Conventional subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) cannot meet patients’ needs owing to the side effects caused by both the use of conventio...
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doaj-9aa14c118f144bb9b55f8849a58601b82020-11-24T21:50:44ZengMDPI AGPharmaceutics1999-49232019-10-01111156310.3390/pharmaceutics11110563pharmaceutics11110563Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis ImmunotherapyQingliang Kong0Kouki Higasijima1Rie Wakabayashi2Yoshiro Tahara3Momoko Kitaoka4Hiroki Obayashi5Yanting Hou6Noriho Kamiya7Masahiro Goto8Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanSchool of Pharmacy, Shenyang Pharmaceutical University, No.103, Wenhua Road, Shenyang 110016, ChinaDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, JapanJapanese cedar pollinosis is a type I allergic disease and has already become a major public health problem in Japan. Conventional subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) cannot meet patients’ needs owing to the side effects caused by both the use of conventional whole antigen molecules in the pollen extract and the administration routes. To address these issues, a surface-modified antigen and transcutaneous administration route are introduced in this research. First, the pollen extract (PE) was conjugated to galactomannan (PE-GM) to mask immunoglobulin E (IgE)-binding epitopes in the PE to avoid side effects. Second, as a safer alternative to SCIT and SLIT, transcutaneous immunotherapy (TCIT) with a solid-in-oil (S/O) nanodispersion system carrying PE-GM was proposed. Hydrophilic PE-GM was efficiently delivered through mouse skin using S/O nanodispersions, reducing the antibody secretion and modifying the type 1 T helper (Th1)/ type 2 T helper (Th2) balance in the mouse model, thereby demonstrating the potential to alleviate Japanese cedar pollinosis.https://www.mdpi.com/1999-4923/11/11/563japanese cedar pollinosisnanocarrierpollen extract-galactomannan conjugatesolid-in-oil nanodispersionstranscutaneous immunotherapyvaccine |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qingliang Kong Kouki Higasijima Rie Wakabayashi Yoshiro Tahara Momoko Kitaoka Hiroki Obayashi Yanting Hou Noriho Kamiya Masahiro Goto |
spellingShingle |
Qingliang Kong Kouki Higasijima Rie Wakabayashi Yoshiro Tahara Momoko Kitaoka Hiroki Obayashi Yanting Hou Noriho Kamiya Masahiro Goto Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy Pharmaceutics japanese cedar pollinosis nanocarrier pollen extract-galactomannan conjugate solid-in-oil nanodispersions transcutaneous immunotherapy vaccine |
author_facet |
Qingliang Kong Kouki Higasijima Rie Wakabayashi Yoshiro Tahara Momoko Kitaoka Hiroki Obayashi Yanting Hou Noriho Kamiya Masahiro Goto |
author_sort |
Qingliang Kong |
title |
Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy |
title_short |
Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy |
title_full |
Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy |
title_fullStr |
Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy |
title_full_unstemmed |
Transcutaneous Delivery of Immunomodulating Pollen Extract-Galactomannan Conjugate by Solid-in-Oil Nanodispersions for Pollinosis Immunotherapy |
title_sort |
transcutaneous delivery of immunomodulating pollen extract-galactomannan conjugate by solid-in-oil nanodispersions for pollinosis immunotherapy |
publisher |
MDPI AG |
series |
Pharmaceutics |
issn |
1999-4923 |
publishDate |
2019-10-01 |
description |
Japanese cedar pollinosis is a type I allergic disease and has already become a major public health problem in Japan. Conventional subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) cannot meet patients’ needs owing to the side effects caused by both the use of conventional whole antigen molecules in the pollen extract and the administration routes. To address these issues, a surface-modified antigen and transcutaneous administration route are introduced in this research. First, the pollen extract (PE) was conjugated to galactomannan (PE-GM) to mask immunoglobulin E (IgE)-binding epitopes in the PE to avoid side effects. Second, as a safer alternative to SCIT and SLIT, transcutaneous immunotherapy (TCIT) with a solid-in-oil (S/O) nanodispersion system carrying PE-GM was proposed. Hydrophilic PE-GM was efficiently delivered through mouse skin using S/O nanodispersions, reducing the antibody secretion and modifying the type 1 T helper (Th1)/ type 2 T helper (Th2) balance in the mouse model, thereby demonstrating the potential to alleviate Japanese cedar pollinosis. |
topic |
japanese cedar pollinosis nanocarrier pollen extract-galactomannan conjugate solid-in-oil nanodispersions transcutaneous immunotherapy vaccine |
url |
https://www.mdpi.com/1999-4923/11/11/563 |
work_keys_str_mv |
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