Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus
Background: Lymphoid tissue is the first to react to stress, which manifests as characteristic morphological manifestations, based on which it is possible to study the duration of damage. Aims: Study of the dynamics of changes in the histomorphometric characteristics of the rat thymus during regener...
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doaj-9aaa3ae700614b3f8a48c803a6fec34a2021-07-08T04:22:26ZrusEco-vectorСудебная Медицина2411-87292409-41612021-07-01729610010.17816/fm401330Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymusAirat A. Khalikov0https://orcid.org/0000-0003-1045-5677Evgeny M. Kildyushov1https://orcid.org/0000-0001-7571-0312Kirill O. Kuznetsov2https://orcid.org/0000-0002-2405-1801Dmitry S. Komlev3https://orcid.org/0000-0003-4180-5326Gulnaz R. Rahmatullina4https://orcid.org/0000-0001-9509-5978Bashkir State Medical UniversityN.I. Pirogov Russian National Research Medical UniversityBashkir State Medical UniversityVolga-Ural Bureau of Forensic MedicineVolga-Ural Bureau of Forensic MedicineBackground: Lymphoid tissue is the first to react to stress, which manifests as characteristic morphological manifestations, based on which it is possible to study the duration of damage. Aims: Study of the dynamics of changes in the histomorphometric characteristics of the rat thymus during regeneration of the musculocutaneous tissue during mechanical thigh trauma. Material and methods: In total, 84 sexually mature rats were enrolled. These were divided into two groups (experiment and control), which were further classified into four subgroups depending on the time elapsed since the injury (1, 3, 15, and 25 days). Mechanical damage was simulated using a setup that transferred kinetic energy (comparable to a 5.6-mm rifle bullet) to tissues. Subsequently, the animals were removed from the experiment by decapitation; their thymus was removed and microscopically examined. In arbitrary units, the total area of the lobules as well as the area of the cortical and cerebral zones was determined. Results: Our research results indicate different phases of changes in the histomorphometric characteristics of the thymus, which correspond to the course of the wound and can be used in forensic practice to determine the duration of the injury. Conclusions: Mechanical trauma, considered as a stress reaction, causes involutive changes in the thymus and is characterized by changes in the area of the thymus cortex and medulla as well as regular dynamics of changes in the thymic lobules at different stages of wound regeneration.https://for-medex.ru/jour/article/viewFile/401/375mechanical injurythymusdetermination of the duration of injurymechanical injury |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
Airat A. Khalikov Evgeny M. Kildyushov Kirill O. Kuznetsov Dmitry S. Komlev Gulnaz R. Rahmatullina |
spellingShingle |
Airat A. Khalikov Evgeny M. Kildyushov Kirill O. Kuznetsov Dmitry S. Komlev Gulnaz R. Rahmatullina Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus Судебная Медицина mechanical injury thymus determination of the duration of injury mechanical injury |
author_facet |
Airat A. Khalikov Evgeny M. Kildyushov Kirill O. Kuznetsov Dmitry S. Komlev Gulnaz R. Rahmatullina |
author_sort |
Airat A. Khalikov |
title |
Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
title_short |
Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
title_full |
Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
title_fullStr |
Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
title_full_unstemmed |
Possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
title_sort |
possibility of determining injury duration based on changes in histomorphometric characteristics of the thymus |
publisher |
Eco-vector |
series |
Судебная Медицина |
issn |
2411-8729 2409-4161 |
publishDate |
2021-07-01 |
description |
Background: Lymphoid tissue is the first to react to stress, which manifests as characteristic morphological manifestations, based on which it is possible to study the duration of damage. Aims: Study of the dynamics of changes in the histomorphometric characteristics of the rat thymus during regeneration of the musculocutaneous tissue during mechanical thigh trauma. Material and methods: In total, 84 sexually mature rats were enrolled. These were divided into two groups (experiment and control), which were further classified into four subgroups depending on the time elapsed since the injury (1, 3, 15, and 25 days). Mechanical damage was simulated using a setup that transferred kinetic energy (comparable to a 5.6-mm rifle bullet) to tissues. Subsequently, the animals were removed from the experiment by decapitation; their thymus was removed and microscopically examined. In arbitrary units, the total area of the lobules as well as the area of the cortical and cerebral zones was determined. Results: Our research results indicate different phases of changes in the histomorphometric characteristics of the thymus, which correspond to the course of the wound and can be used in forensic practice to determine the duration of the injury. Conclusions: Mechanical trauma, considered as a stress reaction, causes involutive changes in the thymus and is characterized by changes in the area of the thymus cortex and medulla as well as regular dynamics of changes in the thymic lobules at different stages of wound regeneration. |
topic |
mechanical injury thymus determination of the duration of injury mechanical injury |
url |
https://for-medex.ru/jour/article/viewFile/401/375 |
work_keys_str_mv |
AT airatakhalikov possibilityofdetermininginjurydurationbasedonchangesinhistomorphometriccharacteristicsofthethymus AT evgenymkildyushov possibilityofdetermininginjurydurationbasedonchangesinhistomorphometriccharacteristicsofthethymus AT kirillokuznetsov possibilityofdetermininginjurydurationbasedonchangesinhistomorphometriccharacteristicsofthethymus AT dmitryskomlev possibilityofdetermininginjurydurationbasedonchangesinhistomorphometriccharacteristicsofthethymus AT gulnazrrahmatullina possibilityofdetermininginjurydurationbasedonchangesinhistomorphometriccharacteristicsofthethymus |
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1721314188591104000 |