Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery
This study describes the assessment of mucosal adjuvant activity of a squalene-based nanoemulsion (SQ@NE) following intravaginal delivery in mice. After immunization, a high level of recruitment of CD11b/c+ granulocytes and F4/80+ macrophages was observed in the vaginal mucosal tissues of the mice i...
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doaj-9acf3877c1434408a7cdb9c2dc8004f02021-09-05T04:38:42ZengElsevierBiomedicine & Pharmacotherapy0753-33222021-09-01141111799Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal deliveryHui-Min Ho0Chiung-Yi Huang1Yu-Jhen Cheng2I-Hua Chen3Shih-Jen Liu4Chung-Hsiung Huang5Ming-Hsi Huang6National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, TaiwanNational Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Food Science, National Taiwan Ocean University, Keelung 20224, Taiwan; Correspondence to: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan.National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli 35053, Taiwan; Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan; Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan; Correspondence to: National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan.This study describes the assessment of mucosal adjuvant activity of a squalene-based nanoemulsion (SQ@NE) following intravaginal delivery in mice. After immunization, a high level of recruitment of CD11b/c+ granulocytes and F4/80+ macrophages was observed in the vaginal mucosal tissues of the mice immunized with a model protein ovalbumin (OVA) formulated with SQ@NE, and then downstream regulated the expression of MHC II and costimulatory molecules CD40 and CD86 on CD11c+ cells harvested from the associated draining lymph node. With respect to cytotoxic T lymphocyte immunity, the mice immunized with SQ@NE-formulated OVA elicited a high population of OVA-specific CD8+ cells in the spleen and increased the secretion of IFN-γ, IL-2 and IL-17 from OVA-restimulated splenocytes compared with those immunized with OVA alone. By studying in vivo fluorescence imaging and B-cell immunoassays, we discovered how SQ@NE prolongs the retention of antigen depots at the mucosal membrane of the immune inductive site and allows them to properly drive the production of antibodies. The data demonstrated that SQ@NE prolonged fluorescence-labeled OVA retention at the genital tract and augmented the production of OVA-specific IgG in sera and IgA in vaginal washes. These results indicate that SQ@NE is a promising vaginal adjuvant for the induction of both mucosal and systemic immune responses, a feature that provides implications for the development of a mucosal vaccine against genital infections and sexually transmitted diseases.http://www.sciencedirect.com/science/article/pii/S0753332221005813Mucosal adjuvantNanoemulsionSqualeneVaccineVaginal delivery |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hui-Min Ho Chiung-Yi Huang Yu-Jhen Cheng I-Hua Chen Shih-Jen Liu Chung-Hsiung Huang Ming-Hsi Huang |
spellingShingle |
Hui-Min Ho Chiung-Yi Huang Yu-Jhen Cheng I-Hua Chen Shih-Jen Liu Chung-Hsiung Huang Ming-Hsi Huang Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery Biomedicine & Pharmacotherapy Mucosal adjuvant Nanoemulsion Squalene Vaccine Vaginal delivery |
author_facet |
Hui-Min Ho Chiung-Yi Huang Yu-Jhen Cheng I-Hua Chen Shih-Jen Liu Chung-Hsiung Huang Ming-Hsi Huang |
author_sort |
Hui-Min Ho |
title |
Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
title_short |
Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
title_full |
Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
title_fullStr |
Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
title_full_unstemmed |
Squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
title_sort |
squalene nanoemulsion reinforces mucosal and immunological fingerprints following intravaginal delivery |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2021-09-01 |
description |
This study describes the assessment of mucosal adjuvant activity of a squalene-based nanoemulsion (SQ@NE) following intravaginal delivery in mice. After immunization, a high level of recruitment of CD11b/c+ granulocytes and F4/80+ macrophages was observed in the vaginal mucosal tissues of the mice immunized with a model protein ovalbumin (OVA) formulated with SQ@NE, and then downstream regulated the expression of MHC II and costimulatory molecules CD40 and CD86 on CD11c+ cells harvested from the associated draining lymph node. With respect to cytotoxic T lymphocyte immunity, the mice immunized with SQ@NE-formulated OVA elicited a high population of OVA-specific CD8+ cells in the spleen and increased the secretion of IFN-γ, IL-2 and IL-17 from OVA-restimulated splenocytes compared with those immunized with OVA alone. By studying in vivo fluorescence imaging and B-cell immunoassays, we discovered how SQ@NE prolongs the retention of antigen depots at the mucosal membrane of the immune inductive site and allows them to properly drive the production of antibodies. The data demonstrated that SQ@NE prolonged fluorescence-labeled OVA retention at the genital tract and augmented the production of OVA-specific IgG in sera and IgA in vaginal washes. These results indicate that SQ@NE is a promising vaginal adjuvant for the induction of both mucosal and systemic immune responses, a feature that provides implications for the development of a mucosal vaccine against genital infections and sexually transmitted diseases. |
topic |
Mucosal adjuvant Nanoemulsion Squalene Vaccine Vaginal delivery |
url |
http://www.sciencedirect.com/science/article/pii/S0753332221005813 |
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