Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum
The secretory pathway in Plasmodium falciparum has evolved to transport proteins to the host cell membrane and to an endosymbiotic organelle, the apicoplast. The latter can occur via the ER or the ER-Golgi route. Here, we study these three routes using proteins Erythrocyte Membrane Protein-1 (PfEMP1...
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doaj-9aeb5d9b9b4842b39b00812141ccdc9b2020-11-24T22:52:05ZengPeerJ Inc.PeerJ2167-83592017-04-015e312810.7717/peerj.3128Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparumRahul Chaudhari0Vishakha Dey1Aishwarya Narayan2Shobhona Sharma3Swati Patankar4Department of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai, Maharashtra, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai, Maharashtra, IndiaDepartment of Biological Sciences, Tata Institute of Fundamental Research, Mumbai, Maharashtra, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology, Bombay, Mumbai, Maharashtra, IndiaThe secretory pathway in Plasmodium falciparum has evolved to transport proteins to the host cell membrane and to an endosymbiotic organelle, the apicoplast. The latter can occur via the ER or the ER-Golgi route. Here, we study these three routes using proteins Erythrocyte Membrane Protein-1 (PfEMP1), Acyl Carrier Protein (ACP) and glutathione peroxidase-like thioredoxin peroxidase (PfTPxGl) and inhibitors of vesicular transport. As expected, the G protein-dependent vesicular fusion inhibitor AlF4− and microtubule destabilizing drug vinblastine block the trafficking of PfEMP-1, a protein secreted to the host cell membrane. However, while both PfTPxGl and ACP are targeted to the apicoplast, only ACP trafficking remains unaffected by these treatments. This implies that G protein-dependent vesicles do not play a role in classical apicoplast protein targeting. Unlike the soluble protein ACP, we show that PfTPxGl is localized to the outermost membrane of the apicoplast. Thus, the parasite apicoplast acquires proteins via two different pathways: first, the vesicular trafficking pathway appears to handle not only secretory proteins, but an apicoplast membrane protein, PfTPxGl; second, trafficking of apicoplast luminal proteins appear to be independent of G protein-coupled vesicles.https://peerj.com/articles/3128.pdfApicoplastAcyl-carrier protein (ACP-GFP)P. falciparumGlutathione peroxidase like thioredoxin peroxidase (PfTPxGl)Protein trafficking |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rahul Chaudhari Vishakha Dey Aishwarya Narayan Shobhona Sharma Swati Patankar |
spellingShingle |
Rahul Chaudhari Vishakha Dey Aishwarya Narayan Shobhona Sharma Swati Patankar Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum PeerJ Apicoplast Acyl-carrier protein (ACP-GFP) P. falciparum Glutathione peroxidase like thioredoxin peroxidase (PfTPxGl) Protein trafficking |
author_facet |
Rahul Chaudhari Vishakha Dey Aishwarya Narayan Shobhona Sharma Swati Patankar |
author_sort |
Rahul Chaudhari |
title |
Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum |
title_short |
Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum |
title_full |
Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum |
title_fullStr |
Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum |
title_full_unstemmed |
Membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite Plasmodium falciparum |
title_sort |
membrane and luminal proteins reach the apicoplast by different trafficking pathways in the malaria parasite plasmodium falciparum |
publisher |
PeerJ Inc. |
series |
PeerJ |
issn |
2167-8359 |
publishDate |
2017-04-01 |
description |
The secretory pathway in Plasmodium falciparum has evolved to transport proteins to the host cell membrane and to an endosymbiotic organelle, the apicoplast. The latter can occur via the ER or the ER-Golgi route. Here, we study these three routes using proteins Erythrocyte Membrane Protein-1 (PfEMP1), Acyl Carrier Protein (ACP) and glutathione peroxidase-like thioredoxin peroxidase (PfTPxGl) and inhibitors of vesicular transport. As expected, the G protein-dependent vesicular fusion inhibitor AlF4− and microtubule destabilizing drug vinblastine block the trafficking of PfEMP-1, a protein secreted to the host cell membrane. However, while both PfTPxGl and ACP are targeted to the apicoplast, only ACP trafficking remains unaffected by these treatments. This implies that G protein-dependent vesicles do not play a role in classical apicoplast protein targeting. Unlike the soluble protein ACP, we show that PfTPxGl is localized to the outermost membrane of the apicoplast. Thus, the parasite apicoplast acquires proteins via two different pathways: first, the vesicular trafficking pathway appears to handle not only secretory proteins, but an apicoplast membrane protein, PfTPxGl; second, trafficking of apicoplast luminal proteins appear to be independent of G protein-coupled vesicles. |
topic |
Apicoplast Acyl-carrier protein (ACP-GFP) P. falciparum Glutathione peroxidase like thioredoxin peroxidase (PfTPxGl) Protein trafficking |
url |
https://peerj.com/articles/3128.pdf |
work_keys_str_mv |
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