Activity of compounds containing echinochrome A against herpes simplex virus type 2 <i>in vitro</i> and <i>in vivo</i>

• Main Authors: N. V. Krylova, I. A. Leneva, S. A. Fedoreev, L. K. Ebralidze, N. P. Mishchenko, E. V. Vasileva, I. N. Falynskova, O. V. Iunikhina, V. F. Lavrov, O. A. Svitich
• Format: Article
• Language: Russian
• Published: Central Research Institute for Epidemiology 2019-12-01
• Series: Журнал микробиологии, эпидемиологии и иммунобиологии
• Subjects: echinochrome a; antioxidant composition; herpes simplex virus type 2; antiviral activity
• Online Access: https://microbiol.elpub.ru/jour/article/view/496
• ISSN: 0372-9311; 2686-7613

The aim of the work was to study the activity of echinochrome A, a naphthoquinoid pigment from sea urchins, and its antioxidant composition against herpes simplex virus type 2 (HSV-2) in vitro and in vivo.Materials and methods. Strain HSV-2 (G ATCC VR-734) was grown in Vero cells. The cytotoxic and anti-HSV-2 activity of the compounds was assessed in vitro by the cell viability and by cytopathic effect inhibition of virus using MTT test. The efficacy of compounds in mice model of vaginitis caused by HSV-2 was determined by the average lifetime, body weight and viral load changes.Results and discussion. The antioxidant composition (echinochrome A, ascorbic acid and α-tocopherol (5:5:1)), showed a higher antiviral efficacy than echinochrome A alone. Oral administration of the antioxidant composition protected 90% of the infected mice against death and reduced vaginal viral loads. The antiviral activity of echinochrome A and the antioxidant composition is probably due to the virus-inhibiting activity of the compounds and their antioxidant properties.Conclusion. The results obtained allow considering the tested compounds as promising agents with antiviral properties.