Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation
Introduction: Coronal and apical leakage still remains one of the most important cause for endodontic failure in spite of the presence of advanced endodontic materials. The cause may attribute to different filling techniques, physical and chemical properties of sealers and presence or absence of...
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doaj-9b0a08bdd4b84e98ad9c967c344d3baa2020-11-25T03:15:37ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2015-12-01912ZC13ZC1710.7860/JCDR/2015/15796.6931Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye PreparationRachit Mathur0Medhavi Sharma1Deepak Sharma2Deepak Raisingani3Suchita Vishnoi4Deepika Singhal5Shekhar Grover6Senior Lecturer, Department of Endodontics, Mahatma Gandhi Dental College, Jaipur, Rajasthan, India.Reader, Department of Endodontics, Mahatma Gandhi Dental College, Jaipur, Rajasthan, India.Professor and HOD, Department of Endodontics, Jaipur Dental College, Jaipur, Rajasthan, India.Professor and HOD, Department of Endodontics, Mahatma Gandhi Dental College, Jaipur, Rajasthan, India.Senior Lecturer, Department of Endodontics, Mahatma Gandhi Dental College, Jaipur, Rajasthan, India.Tutor, Department of Endodontics, ESIC HOSPITAL, New Delhi, India.Senior Lecturer, Department of Preventive and Community Dentistry, MAIDC, New Delhi, India.Introduction: Coronal and apical leakage still remains one of the most important cause for endodontic failure in spite of the presence of advanced endodontic materials. The cause may attribute to different filling techniques, physical and chemical properties of sealers and presence or absence of smear layer assessment of coronal or apical leakage is used as a research method to compare the sealing ability of different techniques and endodontic materials. Aim: To compare the coronal bacterial leakage using methylene blue in four different obturation techniques after protaper hand instrumentation. Materials and Methods: Ninety extracted single-rooted teeth were instrumented to an apical preparation size F3 Protaper hand files. Twenty teeth were randomly obturated with lateral compaction, 20 with vertical compaction, 20 with combination of vertical and lateral compaction and 20 with Thermafil. Ten teeth were used for positive and negative controls (five teeth in each group). Teeth were kept in 100% humidity for 90 days, and then subjected coronally to Proteus vulgaris for 21 days to assess bacterial leakage. After bacterial challenge, methylene blue was placed coronally for another 21 days, and then scoring was done according to depth of dye leakage. Chi-square test was done for statistical analysis. Results: Leakage as observed with combination of vertical and lateral compaction was significantly less than vertical compaction, lateral compaction and thermafil carriers during bacterial challenge. However, when dye was used it also showed statistically significant results with thermafil carriers showing the least leakage in comparison to vertical condensation, lateral condensation and combined groups. Conclusion: The study concludes that two different methods i.e. bacterial and dye leakage revealed considerable variation on the same substrate Thus, due to the presence of variability among the results obtained by two different analytical methods used in the present study, the study emphasizes the need for standardization of methods as the lack of standardization hinders the comparison of different endodontic filling techniques.https://jcdr.net/articles/PDF/6931/15796_CE[Ra1]_F(P)_Re-Format_PF1(AGAK)_PFA(AK)_PF2(PAG).pdfmicroleakagelateral compactionthermafil carrierwarm vertical compactionproteus vulgaristubliseal |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rachit Mathur Medhavi Sharma Deepak Sharma Deepak Raisingani Suchita Vishnoi Deepika Singhal Shekhar Grover |
spellingShingle |
Rachit Mathur Medhavi Sharma Deepak Sharma Deepak Raisingani Suchita Vishnoi Deepika Singhal Shekhar Grover Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation Journal of Clinical and Diagnostic Research microleakage lateral compaction thermafil carrier warm vertical compaction proteus vulgaris tubliseal |
author_facet |
Rachit Mathur Medhavi Sharma Deepak Sharma Deepak Raisingani Suchita Vishnoi Deepika Singhal Shekhar Grover |
author_sort |
Rachit Mathur |
title |
Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation |
title_short |
Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation |
title_full |
Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation |
title_fullStr |
Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation |
title_full_unstemmed |
Evaluation of Coronal Leakage Following Different Obturation Techniques and in-vitro Evalution Using Methylene Blue Dye Preparation |
title_sort |
evaluation of coronal leakage following different obturation techniques and in-vitro evalution using methylene blue dye preparation |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2015-12-01 |
description |
Introduction: Coronal and apical leakage still remains one of
the most important cause for endodontic failure in spite of the
presence of advanced endodontic materials. The cause may
attribute to different filling techniques, physical and chemical
properties of sealers and presence or absence of smear layer
assessment of coronal or apical leakage is used as a research
method to compare the sealing ability of different techniques
and endodontic materials.
Aim: To compare the coronal bacterial leakage using methylene
blue in four different obturation techniques after protaper hand
instrumentation.
Materials and Methods: Ninety extracted single-rooted teeth
were instrumented to an apical preparation size F3 Protaper
hand files. Twenty teeth were randomly obturated with lateral
compaction, 20 with vertical compaction, 20 with combination
of vertical and lateral compaction and 20 with Thermafil. Ten
teeth were used for positive and negative controls (five teeth
in each group). Teeth were kept in 100% humidity for 90 days,
and then subjected coronally to Proteus vulgaris for 21 days to
assess bacterial leakage. After bacterial challenge, methylene
blue was placed coronally for another 21 days, and then scoring
was done according to depth of dye leakage. Chi-square test
was done for statistical analysis.
Results: Leakage as observed with combination of vertical
and lateral compaction was significantly less than vertical
compaction, lateral compaction and thermafil carriers during
bacterial challenge. However, when dye was used it also showed
statistically significant results with thermafil carriers showing
the least leakage in comparison to vertical condensation, lateral
condensation and combined groups.
Conclusion: The study concludes that two different methods
i.e. bacterial and dye leakage revealed considerable variation
on the same substrate Thus, due to the presence of variability
among the results obtained by two different analytical methods
used in the present study, the study emphasizes the need for
standardization of methods as the lack of standardization
hinders the comparison of different endodontic filling
techniques. |
topic |
microleakage lateral compaction thermafil carrier warm vertical compaction proteus vulgaris tubliseal |
url |
https://jcdr.net/articles/PDF/6931/15796_CE[Ra1]_F(P)_Re-Format_PF1(AGAK)_PFA(AK)_PF2(PAG).pdf |
work_keys_str_mv |
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