Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition
Dermatophytosis is caused by a group of pathogenic fungi namely, dermatophytes, is among the most prevalent infectious diseases worldwide. Azole drugs are widely used in the treatment of dermatomycosis, but can cause various side effects and drug resistance to the patients. Hence, for solving this p...
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Sociedade Brasileira de Microbiologia
2012-06-01
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doaj-9b0da6052f114fdba9a1a3ccdc688e012020-11-24T22:40:15ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052012-06-0143278679210.1590/S1517-83822012000200044Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro conditionFarzad AalaUmi Kalsom YusufFarida JamalSassan RezaieDermatophytosis is caused by a group of pathogenic fungi namely, dermatophytes, is among the most prevalent infectious diseases worldwide. Azole drugs are widely used in the treatment of dermatomycosis, but can cause various side effects and drug resistance to the patients. Hence, for solving this problem can be used from the plant extract as alternative for chemical drugs. Allicin is a pure bioactive compound isolated from garlic was tested for its potential as a treatment of dermatomycosis in this study. This study evaluated the in vitro efficacy of pure allicin against ten isolates of Trichophyton rubrum and the MIC50 and MIC90 ranged from 0.78-12.5 µg/ml for allicin. The results revealed that the order of efficacy based on the MICs values, all isolates showed almost comparable response to allicin and ketoconazole except for some isolates, at 28 ºC for both 7 and 10 days incubation. Mann-Whitney test indicate that MICs at 7 days incubation was not observed a significant difference between the effects of allicin and ketoconazole (p > 0.05), but MICs at 10 days incubation, a significant difference was observed (p < 0.05). On the other side, time kill studies revealed that allicin used its fungicidal activity within 12-24 h of management in vitro as well as ketoconazole. In conclusion, allicin showed very good potential as an antifungal compound against mycoses-causing dermatophytes, almost the same as the synthetic drug ketoconazole. Therefore, this antifungal agent appears to be effective, safe and suitable alternative for the treatment of dermatomycosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200044AllicinAntifungal drugsDermatophytesMIC (minimal inhibitory concentration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Farzad Aala Umi Kalsom Yusuf Farida Jamal Sassan Rezaie |
spellingShingle |
Farzad Aala Umi Kalsom Yusuf Farida Jamal Sassan Rezaie Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition Brazilian Journal of Microbiology Allicin Antifungal drugs Dermatophytes MIC (minimal inhibitory concentration |
author_facet |
Farzad Aala Umi Kalsom Yusuf Farida Jamal Sassan Rezaie |
author_sort |
Farzad Aala |
title |
Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
title_short |
Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
title_full |
Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
title_fullStr |
Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
title_full_unstemmed |
Antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
title_sort |
antimicrobial effects of allicin and ketoconazole on trichophyton rubrum under in vitro condition |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2012-06-01 |
description |
Dermatophytosis is caused by a group of pathogenic fungi namely, dermatophytes, is among the most prevalent infectious diseases worldwide. Azole drugs are widely used in the treatment of dermatomycosis, but can cause various side effects and drug resistance to the patients. Hence, for solving this problem can be used from the plant extract as alternative for chemical drugs. Allicin is a pure bioactive compound isolated from garlic was tested for its potential as a treatment of dermatomycosis in this study. This study evaluated the in vitro efficacy of pure allicin against ten isolates of Trichophyton rubrum and the MIC50 and MIC90 ranged from 0.78-12.5 µg/ml for allicin. The results revealed that the order of efficacy based on the MICs values, all isolates showed almost comparable response to allicin and ketoconazole except for some isolates, at 28 ºC for both 7 and 10 days incubation. Mann-Whitney test indicate that MICs at 7 days incubation was not observed a significant difference between the effects of allicin and ketoconazole (p > 0.05), but MICs at 10 days incubation, a significant difference was observed (p < 0.05). On the other side, time kill studies revealed that allicin used its fungicidal activity within 12-24 h of management in vitro as well as ketoconazole. In conclusion, allicin showed very good potential as an antifungal compound against mycoses-causing dermatophytes, almost the same as the synthetic drug ketoconazole. Therefore, this antifungal agent appears to be effective, safe and suitable alternative for the treatment of dermatomycosis. |
topic |
Allicin Antifungal drugs Dermatophytes MIC (minimal inhibitory concentration |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200044 |
work_keys_str_mv |
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