Phytochemical Profile of <i>Asplenium aethiopicum </i>(Burm. f.) Becherer Using HPTLC

• Main Authors: Marimuthu alias Antonysamy Johnson, Janarthanan Gowtham, Narayanan Janakiraman, Tharmaraj Renisheya Joy Jeba Malar, Janaina E. Rocha, Henrique D. M. Coutinho
• Format: Article
• Language: English
• Published: MDPI AG 2020-01-01
• Series: Separations
• Subjects: asplenium aethiopicum; hptlc; flavonoids; alkaloids; tannins
• Online Access: https://www.mdpi.com/2297-8739/7/1/8

<b>: </b>The present study was aimed to validate the phenolic, flavonoids, alkaloids and tannins profile of <i>Aspleniuma ethiopicum </i>(Burm. f.) Becherer methanolic extracts using HPTLC (High-performance thin-layer chromatography). The chromatographic separation was performed using the standard method. The separation methodology was optimized and phytoconstituents of <i>A. aethiopicum </i>methanolic extracts were separated. The methanolic extract of <i>A. aethiopicum</i> showed several bands identified as known phenolic compounds. The obtained flavonoids profiles were identified. HPTLC alkaloids profile of <i>A. aethiopicum</i> revealed 11 types of alkaloids. The tannin profile of methanolic extracts of <i>A. aethiopicum</i> demonstrated 11 different types of tannins. The observed profiles will be used as phytomarker to identify the chemical constituents of <i>A. aethiopicum </i>methanolic extracts. These profiles will act as a fingerprint of <i>A. aethiopicum</i> and help us to distinguish from its adulterants. The observed profile will help us to identify the crude drugs and improve the therapeutic potentials of <i>A. aethiopicum</i>.