CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity wil...
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doaj-9b1e2f427ea741b7b65d862338ed37802020-11-25T03:36:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-11-011110.3389/fpls.2020.584151584151CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural AdvancementAnindya Bandyopadhyay0Nagesh Kancharla1Vivek S. Javalkote2Santanu Dasgupta3Thomas P. Brutnell4Thomas P. Brutnell5Reliance Industries Ltd., R&D-Synthetic Biology, Navi Mumbai, IndiaReliance Industries Ltd., R&D-Synthetic Biology, Navi Mumbai, IndiaReliance Industries Ltd., R&D-Synthetic Biology, Navi Mumbai, IndiaReliance Industries Ltd., R&D-Synthetic Biology, Navi Mumbai, IndiaChinese Academy of Agricultural Sciences, Biotechnology Research Institute, Beijing ChinaGateway Biotechnology, Inc., St. Louis, MO, United StatesGlobal population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA, targeting of T-rich regions of the genome where Cas9 is not suitable for use, RNA processing capability facilitating simpler multiplexing, and its ability to generate double strand breaks (DSB) with staggered ends. Many monocot and dicot species have been successfully edited using this Cas12a system and further research is ongoing to improve its efficiency in plants, including improving the temperature stability of the Cas12a enzyme, identifying new variants of Cas12a or synthetically producing Cas12a with flexible PAM sequences. In this review we provide a comparative survey of CRISPR-Cas12a and Cas9, and provide a perspective on applications of CRISPR-Cas12 in agriculture.https://www.frontiersin.org/articles/10.3389/fpls.2020.584151/fullCRISPRCas9Cas12aNHEJbase editingPAM |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anindya Bandyopadhyay Nagesh Kancharla Vivek S. Javalkote Santanu Dasgupta Thomas P. Brutnell Thomas P. Brutnell |
spellingShingle |
Anindya Bandyopadhyay Nagesh Kancharla Vivek S. Javalkote Santanu Dasgupta Thomas P. Brutnell Thomas P. Brutnell CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement Frontiers in Plant Science CRISPR Cas9 Cas12a NHEJ base editing PAM |
author_facet |
Anindya Bandyopadhyay Nagesh Kancharla Vivek S. Javalkote Santanu Dasgupta Thomas P. Brutnell Thomas P. Brutnell |
author_sort |
Anindya Bandyopadhyay |
title |
CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement |
title_short |
CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement |
title_full |
CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement |
title_fullStr |
CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement |
title_full_unstemmed |
CRISPR-Cas12a (Cpf1): A Versatile Tool in the Plant Genome Editing Tool Box for Agricultural Advancement |
title_sort |
crispr-cas12a (cpf1): a versatile tool in the plant genome editing tool box for agricultural advancement |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2020-11-01 |
description |
Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion from today. To meet the demands of growing, geographically and socio-economically diversified nations, we need to diversity and expand agricultural production. This expansion of agricultural productivity will need to occur under increasing biotic, and environmental constraints driven by climate change. Clustered regularly interspaced short palindromic repeats-site directed nucleases (CRISPR-SDN) and similar genome editing technologies will likely be key enablers to meet future agricultural needs. While the application of CRISPR-Cas9 mediated genome editing has led the way, the use of CRISPR-Cas12a is also increasing significantly for genome engineering of plants. The popularity of the CRISPR-Cas12a, the type V (class-II) system, is gaining momentum because of its versatility and simplified features. These include the use of a small guide RNA devoid of trans-activating crispr RNA, targeting of T-rich regions of the genome where Cas9 is not suitable for use, RNA processing capability facilitating simpler multiplexing, and its ability to generate double strand breaks (DSB) with staggered ends. Many monocot and dicot species have been successfully edited using this Cas12a system and further research is ongoing to improve its efficiency in plants, including improving the temperature stability of the Cas12a enzyme, identifying new variants of Cas12a or synthetically producing Cas12a with flexible PAM sequences. In this review we provide a comparative survey of CRISPR-Cas12a and Cas9, and provide a perspective on applications of CRISPR-Cas12 in agriculture. |
topic |
CRISPR Cas9 Cas12a NHEJ base editing PAM |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2020.584151/full |
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