Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D
Commercial surfactant products derived from animal lungs are used for the treatment of respiratory diseases in premature neonates. These products contain lipids and the hydrophobic surfactant proteins B and C, which help to lower the surface tension in the lungs. Surfactant products are less effecti...
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doaj-9b3920d9a2db473f85a50d538b63aed52020-11-24T23:49:55ZengFrontiers Media S.A.Frontiers in Immunology1664-32242014-12-01510.3389/fimmu.2014.00623118742Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein DDaniela eSalgado0Rainer eFischer1Rainer eFischer2Stefan eSchillberg3Richard M Twyman4Stefan eRasche5Fraunhofer InstituteFraunhofer InstituteRWTHFraunhofer InstituteTRM LtdFraunhofer InstituteCommercial surfactant products derived from animal lungs are used for the treatment of respiratory diseases in premature neonates. These products contain lipids and the hydrophobic surfactant proteins B and C, which help to lower the surface tension in the lungs. Surfactant products are less effective when pulmonary diseases involve inflammatory complications because two hydrophilic surfactant proteins (A and D) are lost during the extraction process, yet surfactant protein D (SP-D) is a component of the innate immune system that helps to reduce lung inflammation. The performance of surfactant products could therefore be improved by supplementing them with an additional source of SP-D. Recombinant SP-D is produced in mammalian cells and bacteria (Escherichia coli), and also experimentally in the yeast Pichia pastoris. Mammalian cells produce full-size SP-D, but the yields are low and the cost of production is high. In contrast, bacteria produce a truncated form of SP-D, which is active in vitro and in vivo, and higher yields can be achieved at a lower cost. We compare the efficiency of production of recombinant SP-D in terms of the total yields achieved in each system and the amount of SP-D needed to meet the global demand for the treatment of pulmonary diseases, using respiratory distress syndrome as a case study.http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00623/fullbiopharmaceuticalspulmonary surfactantRespiratory Distress Syndromerecombinant protein yieldrecombinant surfactant protein Dheterologous production platform |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniela eSalgado Rainer eFischer Rainer eFischer Stefan eSchillberg Richard M Twyman Stefan eRasche |
spellingShingle |
Daniela eSalgado Rainer eFischer Rainer eFischer Stefan eSchillberg Richard M Twyman Stefan eRasche Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D Frontiers in Immunology biopharmaceuticals pulmonary surfactant Respiratory Distress Syndrome recombinant protein yield recombinant surfactant protein D heterologous production platform |
author_facet |
Daniela eSalgado Rainer eFischer Rainer eFischer Stefan eSchillberg Richard M Twyman Stefan eRasche |
author_sort |
Daniela eSalgado |
title |
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D |
title_short |
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D |
title_full |
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D |
title_fullStr |
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D |
title_full_unstemmed |
Comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein D |
title_sort |
comparative evaluation of heterologous production systems for recombinant pulmonary surfactant protein d |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2014-12-01 |
description |
Commercial surfactant products derived from animal lungs are used for the treatment of respiratory diseases in premature neonates. These products contain lipids and the hydrophobic surfactant proteins B and C, which help to lower the surface tension in the lungs. Surfactant products are less effective when pulmonary diseases involve inflammatory complications because two hydrophilic surfactant proteins (A and D) are lost during the extraction process, yet surfactant protein D (SP-D) is a component of the innate immune system that helps to reduce lung inflammation. The performance of surfactant products could therefore be improved by supplementing them with an additional source of SP-D. Recombinant SP-D is produced in mammalian cells and bacteria (Escherichia coli), and also experimentally in the yeast Pichia pastoris. Mammalian cells produce full-size SP-D, but the yields are low and the cost of production is high. In contrast, bacteria produce a truncated form of SP-D, which is active in vitro and in vivo, and higher yields can be achieved at a lower cost. We compare the efficiency of production of recombinant SP-D in terms of the total yields achieved in each system and the amount of SP-D needed to meet the global demand for the treatment of pulmonary diseases, using respiratory distress syndrome as a case study. |
topic |
biopharmaceuticals pulmonary surfactant Respiratory Distress Syndrome recombinant protein yield recombinant surfactant protein D heterologous production platform |
url |
http://journal.frontiersin.org/Journal/10.3389/fimmu.2014.00623/full |
work_keys_str_mv |
AT danielaesalgado comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind AT rainerefischer comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind AT rainerefischer comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind AT stefaneschillberg comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind AT richardmtwyman comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind AT stefanerasche comparativeevaluationofheterologousproductionsystemsforrecombinantpulmonarysurfactantproteind |
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