A Potential ceRNA Network for Neurological Damage in Preterm Infants
Objective. This study is aimed at identifying key genes involved in neurological damage in preterm infants and at determining their potential circRNA-miRNA-mRNA regulatory mechanisms. Methods. Differentially expressed miRNAs, mRNAs, and circRNAs were downloaded from the GEO database. GO and KEGG enr...
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Online Access: | http://dx.doi.org/10.1155/2021/2628824 |
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doaj-9b470fcaa5d84415b880b721d9b356762021-09-06T00:00:17ZengHindawi LimitedBioMed Research International2314-61412021-01-01202110.1155/2021/2628824A Potential ceRNA Network for Neurological Damage in Preterm InfantsJin Huang0Xuejing Liang1Zhenyu Cai2Department of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyDepartment of Obstetrics and GynecologyObjective. This study is aimed at identifying key genes involved in neurological damage in preterm infants and at determining their potential circRNA-miRNA-mRNA regulatory mechanisms. Methods. Differentially expressed miRNAs, mRNAs, and circRNAs were downloaded from the GEO database. GO and KEGG enrichment analyses were used to determine possible relevant functions of differentially expressed mRNAs. The TTRUST database was used to predict differential TF-mRNA regulatory relationships. Then, CircMIR, miRDB, TargetScan and miRTarBase were then used to map circRNA/miRNA-TF/mRNA interaction networks. Finally, GSEA enrichment analysis was performed on the core transcription factors. Results. A total of 640 mRNAs, 139 circRNAs, and 206 differentially expressed miRNAs associated with neurological injury in preterm infants were obtained. Based on the findings of Cytoscape and PPI network analysis, the hsa_circ_0008439-hsa-mir-3665-STAT3-MMP3 regulatory axis was established. GSEA analysis revealed that suppressed expression levels of STAT3 were associated with upregulated oxidative phosphorylation pathways in the neurological injury group of preterm infants. Conclusions. The circRNA-miRNA-TF-mRNA regulatory network of neurological injury in preterm infants can be used to elucidate on the pathogenesis of brain injury and help us with the early detection of brain injury in preterm infants.http://dx.doi.org/10.1155/2021/2628824 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Huang Xuejing Liang Zhenyu Cai |
spellingShingle |
Jin Huang Xuejing Liang Zhenyu Cai A Potential ceRNA Network for Neurological Damage in Preterm Infants BioMed Research International |
author_facet |
Jin Huang Xuejing Liang Zhenyu Cai |
author_sort |
Jin Huang |
title |
A Potential ceRNA Network for Neurological Damage in Preterm Infants |
title_short |
A Potential ceRNA Network for Neurological Damage in Preterm Infants |
title_full |
A Potential ceRNA Network for Neurological Damage in Preterm Infants |
title_fullStr |
A Potential ceRNA Network for Neurological Damage in Preterm Infants |
title_full_unstemmed |
A Potential ceRNA Network for Neurological Damage in Preterm Infants |
title_sort |
potential cerna network for neurological damage in preterm infants |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6141 |
publishDate |
2021-01-01 |
description |
Objective. This study is aimed at identifying key genes involved in neurological damage in preterm infants and at determining their potential circRNA-miRNA-mRNA regulatory mechanisms. Methods. Differentially expressed miRNAs, mRNAs, and circRNAs were downloaded from the GEO database. GO and KEGG enrichment analyses were used to determine possible relevant functions of differentially expressed mRNAs. The TTRUST database was used to predict differential TF-mRNA regulatory relationships. Then, CircMIR, miRDB, TargetScan and miRTarBase were then used to map circRNA/miRNA-TF/mRNA interaction networks. Finally, GSEA enrichment analysis was performed on the core transcription factors. Results. A total of 640 mRNAs, 139 circRNAs, and 206 differentially expressed miRNAs associated with neurological injury in preterm infants were obtained. Based on the findings of Cytoscape and PPI network analysis, the hsa_circ_0008439-hsa-mir-3665-STAT3-MMP3 regulatory axis was established. GSEA analysis revealed that suppressed expression levels of STAT3 were associated with upregulated oxidative phosphorylation pathways in the neurological injury group of preterm infants. Conclusions. The circRNA-miRNA-TF-mRNA regulatory network of neurological injury in preterm infants can be used to elucidate on the pathogenesis of brain injury and help us with the early detection of brain injury in preterm infants. |
url |
http://dx.doi.org/10.1155/2021/2628824 |
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