Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp
Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stre...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-06-01
|
Series: | Frontiers in Physiology |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2021.666363/full |
id |
doaj-9b4cc4d068894b1b8d1d830731a24d08 |
---|---|
record_format |
Article |
spelling |
doaj-9b4cc4d068894b1b8d1d830731a24d082021-06-04T08:33:55ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2021-06-011210.3389/fphys.2021.666363666363Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian CarpWen-bin Liu0Wen-bin Liu1Min-meng Wang2Min-meng Wang3Liu-ye Dai4Liu-ye Dai5Sheng-hua Dong6Sheng-hua Dong7Xiu-dan Yuan8Xiu-dan Yuan9Shu-li Yuan10Shu-li Yuan11Yi Tang12Yi Tang13Jin-hui Liu14Jin-hui Liu15Liang-yue Peng16Liang-yue Peng17Ya-mei Xiao18Ya-mei Xiao19State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, ChinaCollege of Life Sciences, Hunan Normal University, Changsha, ChinaPrevious research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.https://www.frontiersin.org/articles/10.3389/fphys.2021.666363/fullcadmiumtriploid crucian carpimmune responseliver functiontranscriptome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wen-bin Liu Wen-bin Liu Min-meng Wang Min-meng Wang Liu-ye Dai Liu-ye Dai Sheng-hua Dong Sheng-hua Dong Xiu-dan Yuan Xiu-dan Yuan Shu-li Yuan Shu-li Yuan Yi Tang Yi Tang Jin-hui Liu Jin-hui Liu Liang-yue Peng Liang-yue Peng Ya-mei Xiao Ya-mei Xiao |
spellingShingle |
Wen-bin Liu Wen-bin Liu Min-meng Wang Min-meng Wang Liu-ye Dai Liu-ye Dai Sheng-hua Dong Sheng-hua Dong Xiu-dan Yuan Xiu-dan Yuan Shu-li Yuan Shu-li Yuan Yi Tang Yi Tang Jin-hui Liu Jin-hui Liu Liang-yue Peng Liang-yue Peng Ya-mei Xiao Ya-mei Xiao Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp Frontiers in Physiology cadmium triploid crucian carp immune response liver function transcriptome |
author_facet |
Wen-bin Liu Wen-bin Liu Min-meng Wang Min-meng Wang Liu-ye Dai Liu-ye Dai Sheng-hua Dong Sheng-hua Dong Xiu-dan Yuan Xiu-dan Yuan Shu-li Yuan Shu-li Yuan Yi Tang Yi Tang Jin-hui Liu Jin-hui Liu Liang-yue Peng Liang-yue Peng Ya-mei Xiao Ya-mei Xiao |
author_sort |
Wen-bin Liu |
title |
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp |
title_short |
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp |
title_full |
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp |
title_fullStr |
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp |
title_full_unstemmed |
Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp |
title_sort |
enhanced immune response improves resistance to cadmium stress in triploid crucian carp |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Physiology |
issn |
1664-042X |
publishDate |
2021-06-01 |
description |
Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution. |
topic |
cadmium triploid crucian carp immune response liver function transcriptome |
url |
https://www.frontiersin.org/articles/10.3389/fphys.2021.666363/full |
work_keys_str_mv |
AT wenbinliu enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT wenbinliu enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT minmengwang enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT minmengwang enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT liuyedai enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT liuyedai enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT shenghuadong enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT shenghuadong enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT xiudanyuan enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT xiudanyuan enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT shuliyuan enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT shuliyuan enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT yitang enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT yitang enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT jinhuiliu enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT jinhuiliu enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT liangyuepeng enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT liangyuepeng enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT yameixiao enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp AT yameixiao enhancedimmuneresponseimprovesresistancetocadmiumstressintriploidcruciancarp |
_version_ |
1721397720619417600 |