Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model
Introduction. We examined the murine hepatectomy model of liver regeneration (LR) in the setting of neoadjuvant chemotherapy. Methods. C57BL/6 mice were randomized to receive neoadjuvant intraperitoneal (IP) injections of a control, oxaliplatin (15 mg/kg), or irinotecan (100 mg/Kg or 250 mg/Kg) solu...
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doaj-9b4fc0c3b66b4309a3a463847df0dc8b2020-11-24T23:21:10ZengHindawi LimitedInternational Journal of Hepatology2090-34482090-34562011-01-01201110.4061/2011/490463490463Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine ModelPerry A. Soriano0Nian Liu1Erick Castillo2Brock Foster3Avo Artinyan4Joseph Kim5Wendong Huang6Lawrence D. Wagman7Liver Tumor Program, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USACenter for Gene Regulation and Drug Discovery, Beckman Research Institute, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USALiver Tumor Program, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USALiver Tumor Program, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USALiver Tumor Program, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USALiver Tumor Program, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USACenter for Gene Regulation and Drug Discovery, Beckman Research Institute, City of Hope, 1500 E. Duarte Road, Duarte, CA 91010, USAThe Center for Cancer Prevention and Treatment, St. Joseph Hospital, 1000 West La Veta Avenue, Orange, CA 92868, USAIntroduction. We examined the murine hepatectomy model of liver regeneration (LR) in the setting of neoadjuvant chemotherapy. Methods. C57BL/6 mice were randomized to receive neoadjuvant intraperitoneal (IP) injections of a control, oxaliplatin (15 mg/kg), or irinotecan (100 mg/Kg or 250 mg/Kg) solution. Hepatectomy (70%) was performed 14 days after the final IP treatment. Animals were sacrificed at postoperative day (D) 0, 1, 2, 3, and 7. Liver remnants and serum were collected for analysis. 𝑇-tests for independent samples were used for statistical comparisons. Results. For oxaliplatin, percent LR did not differ at D1 or D2 but was significantly less at D3 (89.0% versus 70.0%, 𝑃=0.048) with no difference on D7 (𝑃=0.21). Irinotecan-treated mice at both dose levels (100 mg/Kg and 250 mg/Kg) showed no significant differences in LR. BrdU incorporation was significantly decreased in oxaliplatin-treated animals (D1,2,3). Conclusions. Neoadjuvant oxaliplatin but not irinotecan impairs early LR in a posthepatectomy murine model which correlates with decreased DNA synthesis.http://dx.doi.org/10.4061/2011/490463 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Perry A. Soriano Nian Liu Erick Castillo Brock Foster Avo Artinyan Joseph Kim Wendong Huang Lawrence D. Wagman |
spellingShingle |
Perry A. Soriano Nian Liu Erick Castillo Brock Foster Avo Artinyan Joseph Kim Wendong Huang Lawrence D. Wagman Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model International Journal of Hepatology |
author_facet |
Perry A. Soriano Nian Liu Erick Castillo Brock Foster Avo Artinyan Joseph Kim Wendong Huang Lawrence D. Wagman |
author_sort |
Perry A. Soriano |
title |
Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model |
title_short |
Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model |
title_full |
Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model |
title_fullStr |
Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model |
title_full_unstemmed |
Oxaliplatin but Not Irinotecan Impairs Posthepatectomy Liver Regeneration in a Murine Model |
title_sort |
oxaliplatin but not irinotecan impairs posthepatectomy liver regeneration in a murine model |
publisher |
Hindawi Limited |
series |
International Journal of Hepatology |
issn |
2090-3448 2090-3456 |
publishDate |
2011-01-01 |
description |
Introduction. We examined the murine hepatectomy model of liver regeneration (LR) in the setting of neoadjuvant chemotherapy. Methods. C57BL/6 mice were randomized to receive neoadjuvant intraperitoneal (IP) injections of a control, oxaliplatin (15 mg/kg), or irinotecan (100 mg/Kg or 250 mg/Kg) solution. Hepatectomy (70%) was performed 14 days after the final IP treatment. Animals were sacrificed at postoperative day (D) 0, 1, 2, 3, and 7. Liver remnants and serum were collected for analysis. 𝑇-tests for independent samples were used for statistical comparisons. Results. For oxaliplatin, percent LR did not differ at D1 or D2 but was significantly less at D3 (89.0% versus 70.0%, 𝑃=0.048) with no difference on D7 (𝑃=0.21). Irinotecan-treated mice at both dose levels (100 mg/Kg and 250 mg/Kg) showed no significant differences in LR. BrdU incorporation was significantly decreased in oxaliplatin-treated animals (D1,2,3). Conclusions. Neoadjuvant oxaliplatin but not irinotecan impairs early LR in a posthepatectomy murine model which correlates with decreased DNA synthesis. |
url |
http://dx.doi.org/10.4061/2011/490463 |
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