Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish
During development, proliferation must be tightly controlled for organs to reach their appropriate size. While the Hippo signaling pathway plays a major role in organ growth control, how it senses and responds to increased cell density is still unclear. In this study, we use the zebrafish lateral li...
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doaj-9b62c3c4bd69442fbb80be607b64ae552021-05-05T00:00:07ZengeLife Sciences Publications LtdeLife2050-084X2015-09-01410.7554/eLife.08201Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafishSobhika Agarwala0Sandra Duquesne1Kun Liu2Anton Boehm3Lin Grimm4Sandra Link5Sabine König6Stefan Eimer7Olaf Ronneberger8Virginie Lecaudey9BIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyDevelopmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Developmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; ZBSA Center for Biological Systems Analysis, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyBIOSS Centre for Biological Signalling Studies, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, Germany; Image Analysis Lab, Institute for Computer Science, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyDevelopmental Biology, Institute for Biology I, Faculty of Biology, Albert Ludwigs University of Freiburg, Freiburg im Breisgau, GermanyDuring development, proliferation must be tightly controlled for organs to reach their appropriate size. While the Hippo signaling pathway plays a major role in organ growth control, how it senses and responds to increased cell density is still unclear. In this study, we use the zebrafish lateral line primordium (LLP), a group of migrating epithelial cells that form sensory organs, to understand how tissue growth is controlled during organ formation. Loss of the cell junction-associated Motin protein Amotl2a leads to overproliferation and bigger LLP, affecting the final pattern of sensory organs. Amotl2a function in the LLP is mediated together by the Hippo pathway effector Yap1 and the Wnt/β-catenin effector Lef1. Our results implicate for the first time the Hippo pathway in size regulation in the LL system. We further provide evidence that the Hippo/Motin interaction is essential to limit tissue size during development.https://elifesciences.org/articles/08201Amotl2Hippoproliferationorgan sizeYap1Lef1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sobhika Agarwala Sandra Duquesne Kun Liu Anton Boehm Lin Grimm Sandra Link Sabine König Stefan Eimer Olaf Ronneberger Virginie Lecaudey |
spellingShingle |
Sobhika Agarwala Sandra Duquesne Kun Liu Anton Boehm Lin Grimm Sandra Link Sabine König Stefan Eimer Olaf Ronneberger Virginie Lecaudey Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish eLife Amotl2 Hippo proliferation organ size Yap1 Lef1 |
author_facet |
Sobhika Agarwala Sandra Duquesne Kun Liu Anton Boehm Lin Grimm Sandra Link Sabine König Stefan Eimer Olaf Ronneberger Virginie Lecaudey |
author_sort |
Sobhika Agarwala |
title |
Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish |
title_short |
Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish |
title_full |
Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish |
title_fullStr |
Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish |
title_full_unstemmed |
Amotl2a interacts with the Hippo effector Yap1 and the Wnt/β-catenin effector Lef1 to control tissue size in zebrafish |
title_sort |
amotl2a interacts with the hippo effector yap1 and the wnt/β-catenin effector lef1 to control tissue size in zebrafish |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2015-09-01 |
description |
During development, proliferation must be tightly controlled for organs to reach their appropriate size. While the Hippo signaling pathway plays a major role in organ growth control, how it senses and responds to increased cell density is still unclear. In this study, we use the zebrafish lateral line primordium (LLP), a group of migrating epithelial cells that form sensory organs, to understand how tissue growth is controlled during organ formation. Loss of the cell junction-associated Motin protein Amotl2a leads to overproliferation and bigger LLP, affecting the final pattern of sensory organs. Amotl2a function in the LLP is mediated together by the Hippo pathway effector Yap1 and the Wnt/β-catenin effector Lef1. Our results implicate for the first time the Hippo pathway in size regulation in the LL system. We further provide evidence that the Hippo/Motin interaction is essential to limit tissue size during development. |
topic |
Amotl2 Hippo proliferation organ size Yap1 Lef1 |
url |
https://elifesciences.org/articles/08201 |
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