Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma
Objective. To investigate the antitumor effect of andrographolide on the ATC cell lines 8505C and CAL62 and to explore the possible mechanism of the effect. Methods. CCK8 and colony formation assays were performed to detect proliferation. Cell migration was tested by scratch assay. Annexin V/PI stai...
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Hindawi Limited
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Series: | Evidence-Based Complementary and Alternative Medicine |
Online Access: | http://dx.doi.org/10.1155/2021/5526581 |
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doaj-9b6fa6fd5349400bb539b02a3b25b0b02021-07-26T00:33:55ZengHindawi LimitedEvidence-Based Complementary and Alternative Medicine1741-42882021-01-01202110.1155/2021/5526581Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid CarcinomaKe Yang0Ke Wu1Jianguo Feng2Ling Yutian3Xin Zhu4Dong Xu5The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital)Objective. To investigate the antitumor effect of andrographolide on the ATC cell lines 8505C and CAL62 and to explore the possible mechanism of the effect. Methods. CCK8 and colony formation assays were performed to detect proliferation. Cell migration was tested by scratch assay. Annexin V/PI staining was used to detect cell apoptosis and cell cycle. Glucose and lactic acid kits were carried out to evaluate the glycolysis level after andrographolide treatment. Western blot was used to detect the changes in the apoptosis-related proteins and glycolysis-related enzymes in both 8505C and CAL62 cells. Results. Treatment with 60 μM andrographolide had significant effects on 8505C and CAL62, including inhibition of proliferation, inhibition of migration, arrest of the cell cycle, promotion of apoptosis, and inhibition of glycolysis. Conclusion. Andrographolide has an antitumor effect and can significantly affect glycolysis in ATC cells.http://dx.doi.org/10.1155/2021/5526581 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ke Yang Ke Wu Jianguo Feng Ling Yutian Xin Zhu Dong Xu |
spellingShingle |
Ke Yang Ke Wu Jianguo Feng Ling Yutian Xin Zhu Dong Xu Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma Evidence-Based Complementary and Alternative Medicine |
author_facet |
Ke Yang Ke Wu Jianguo Feng Ling Yutian Xin Zhu Dong Xu |
author_sort |
Ke Yang |
title |
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma |
title_short |
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma |
title_full |
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma |
title_fullStr |
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma |
title_full_unstemmed |
Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma |
title_sort |
study on the antitumor effect and glycolysis of andrographolide in anaplastic thyroid carcinoma |
publisher |
Hindawi Limited |
series |
Evidence-Based Complementary and Alternative Medicine |
issn |
1741-4288 |
publishDate |
2021-01-01 |
description |
Objective. To investigate the antitumor effect of andrographolide on the ATC cell lines 8505C and CAL62 and to explore the possible mechanism of the effect. Methods. CCK8 and colony formation assays were performed to detect proliferation. Cell migration was tested by scratch assay. Annexin V/PI staining was used to detect cell apoptosis and cell cycle. Glucose and lactic acid kits were carried out to evaluate the glycolysis level after andrographolide treatment. Western blot was used to detect the changes in the apoptosis-related proteins and glycolysis-related enzymes in both 8505C and CAL62 cells. Results. Treatment with 60 μM andrographolide had significant effects on 8505C and CAL62, including inhibition of proliferation, inhibition of migration, arrest of the cell cycle, promotion of apoptosis, and inhibition of glycolysis. Conclusion. Andrographolide has an antitumor effect and can significantly affect glycolysis in ATC cells. |
url |
http://dx.doi.org/10.1155/2021/5526581 |
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