Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae
Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is promising approach to engineer heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed xylose-fermenting yeast SyBE001 by combinatorial fine-tuning the expression of XylA and en...
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doaj-9b82b0a6380c499bbf3d754ed3ccc9ed2020-11-24T22:28:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-10-01610.3389/fmicb.2015.01165149358Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiaeXin eQi0Jian eZha1Gao-Gang eLiu2Weiwen eZhang3Bing-Zhi eLi4Yingjin eYuan5Tianjin UniversityTianjin UniversityTianjin UniversityTianjin UniversityTianjin UniversityTianjin UniversityXylose utilization is one key issue for the bioconversion of lignocelluloses. It is promising approach to engineer heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed xylose-fermenting yeast SyBE001 by combinatorial fine-tuning the expression of XylA and endogenous XKS1. Overexpression of genes RKI1, RPE1, TKL1 and TAL1 in the non-oxidative pentose phosphate pathway in SyBE002 accelerated xylose utilization by 19%. By repetitive adaptation, the xylose utilization rate increased to about 10 folds in strain SyBE003 evolved from SyBE002. Gene expression analysis identified variety of genes with significantly different expressions in pentose phosphate pathway, glycolysis and tricarboxylic acid cycle in SyBE003.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01165/fullSynthetic Biologyyeastevolutionary engineeringXylose isomeraseXylose utilizationXylA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin eQi Jian eZha Gao-Gang eLiu Weiwen eZhang Bing-Zhi eLi Yingjin eYuan |
spellingShingle |
Xin eQi Jian eZha Gao-Gang eLiu Weiwen eZhang Bing-Zhi eLi Yingjin eYuan Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae Frontiers in Microbiology Synthetic Biology yeast evolutionary engineering Xylose isomerase Xylose utilization XylA |
author_facet |
Xin eQi Jian eZha Gao-Gang eLiu Weiwen eZhang Bing-Zhi eLi Yingjin eYuan |
author_sort |
Xin eQi |
title |
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae |
title_short |
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae |
title_full |
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae |
title_fullStr |
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae |
title_full_unstemmed |
Heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in Saccharomyces cerevisiae |
title_sort |
heterologous xylose isomerase pathway and evolutionary engineering improve xylose utilization in saccharomyces cerevisiae |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2015-10-01 |
description |
Xylose utilization is one key issue for the bioconversion of lignocelluloses. It is promising approach to engineer heterologous pathway for xylose utilization in Saccharomyces cerevisiae. Here, we constructed xylose-fermenting yeast SyBE001 by combinatorial fine-tuning the expression of XylA and endogenous XKS1. Overexpression of genes RKI1, RPE1, TKL1 and TAL1 in the non-oxidative pentose phosphate pathway in SyBE002 accelerated xylose utilization by 19%. By repetitive adaptation, the xylose utilization rate increased to about 10 folds in strain SyBE003 evolved from SyBE002. Gene expression analysis identified variety of genes with significantly different expressions in pentose phosphate pathway, glycolysis and tricarboxylic acid cycle in SyBE003. |
topic |
Synthetic Biology yeast evolutionary engineering Xylose isomerase Xylose utilization XylA |
url |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01165/full |
work_keys_str_mv |
AT xineqi heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae AT jianezha heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae AT gaogangeliu heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae AT weiwenezhang heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae AT bingzhieli heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae AT yingjineyuan heterologousxyloseisomerasepathwayandevolutionaryengineeringimprovexyloseutilizationinsaccharomycescerevisiae |
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1725746623029444608 |