Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence
Objective(s): Maternal high-fat diet has been shown to have deleterious effects on the offspring bones. However, there is no study to assess the effects of type and amount of maternal dietary oil in an isocaloric diet, with focus on extra virgin olive oil (EVOO). The objective of the current study w...
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doaj-9b9498058ee84a909770c4ca597c1a172020-11-24T22:39:18ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences 2008-38662008-38742016-12-0119121299130710.22038/ijbms.2016.79157915Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescenceSeyedeh Neda Mousavi0Fariba Koohdani1Mohamadreza Baghaban Eslaminejad2Pantea Izadi3Mohamadreza Eshraghian4Forough Azam Sayahpour5Leila Shafiei Neek6Farzad Shidfar7Department of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran|Department of Biochemistry and Nutrition, School of Medicine, Zanjan University of Medical Sciences, Zanjan, IranDepartment of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Statistics, School of Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, IranDepartment of Sport Physiology, School of Physical Education and Sport Sciences, University of Tehran, Tehran, IranDepartment of Nutrition, School of Health, Colorectal Research Center, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, IranObjective(s): Maternal high-fat diet has been shown to have deleterious effects on the offspring bones. However, there is no study to assess the effects of type and amount of maternal dietary oil in an isocaloric diet, with focus on extra virgin olive oil (EVOO). The objective of the current study was to test the hypothesis that type of maternal dietary oil has more effects than its amount in an isocaloric diet during gestation and lactation on bone genes expression in offspring in adolescence. Materials and Methods: Virgin female C57BL/6 mice were impregnated and fed either the AIN 93G diet (received 16% of calories as soybean oil, as a control diet, or EVOO) or a high fat AIN 93G diet (received 45% of calories as soybean oil or EVOO) from the time of vaginal plug confirmation until offspring’s weaning. Results: After adjusting for the amount of oils, osteoprotegerin/ receptor activator of nuclear factor NF-κB ligand (OPG/RANK-L) and OPG expressions were 6.1- and 2.8-folds higher in offspring born to EVOO compared with soybean oil-fed mothers. OPG, beta-catenin, and OPG/RANK-L expression were 88%, 94%, and 70% lower in offspring born to the 45% oil-fed mothers compared with the 16% group. In contrast, peroxisome proliferator-activated receptor gamma-2 (PPARγ2) gene expression was higher in the 45% oil group, adjusted for the types of oil. Conclusion: Maternal EVOO consumption, but not soybean oil increased osteoblastic gene expression, and high amounts of both oils decreased osteoblastic and increased adipogenic genes expression in adolescent offspring.http://ijbms.mums.ac.ir/article_7915_872136441a6d3030bdc67e9f1558aeaa.pdfDietary oilFetal programmingGestationLactationMouseOlive oilOsteoblastogenesis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Seyedeh Neda Mousavi Fariba Koohdani Mohamadreza Baghaban Eslaminejad Pantea Izadi Mohamadreza Eshraghian Forough Azam Sayahpour Leila Shafiei Neek Farzad Shidfar |
spellingShingle |
Seyedeh Neda Mousavi Fariba Koohdani Mohamadreza Baghaban Eslaminejad Pantea Izadi Mohamadreza Eshraghian Forough Azam Sayahpour Leila Shafiei Neek Farzad Shidfar Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence Iranian Journal of Basic Medical Sciences Dietary oil Fetal programming Gestation Lactation Mouse Olive oil Osteoblastogenesis |
author_facet |
Seyedeh Neda Mousavi Fariba Koohdani Mohamadreza Baghaban Eslaminejad Pantea Izadi Mohamadreza Eshraghian Forough Azam Sayahpour Leila Shafiei Neek Farzad Shidfar |
author_sort |
Seyedeh Neda Mousavi |
title |
Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
title_short |
Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
title_full |
Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
title_fullStr |
Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
title_full_unstemmed |
Extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
title_sort |
extra virgin olive oil in maternal diet increases osteogenic genes expression, but high amounts have deleterious effects on bones in mice offspring at adolescence |
publisher |
Mashhad University of Medical Sciences |
series |
Iranian Journal of Basic Medical Sciences |
issn |
2008-3866 2008-3874 |
publishDate |
2016-12-01 |
description |
Objective(s): Maternal high-fat diet has been shown to have deleterious effects on the offspring bones. However, there is no study to assess the effects of type and amount of maternal dietary oil in an isocaloric diet, with focus on extra virgin olive oil (EVOO). The objective of the current study was to test the hypothesis that type of maternal dietary oil has more effects than its amount in an isocaloric diet during gestation and lactation on bone genes expression in offspring in adolescence. Materials and Methods: Virgin female C57BL/6 mice were impregnated and fed either the AIN 93G diet (received 16% of calories as soybean oil, as a control diet, or EVOO) or a high fat AIN 93G diet (received 45% of calories as soybean oil or EVOO) from the time of vaginal plug confirmation until offspring’s weaning. Results: After adjusting for the amount of oils, osteoprotegerin/ receptor activator of nuclear factor NF-κB ligand (OPG/RANK-L) and OPG expressions were 6.1- and 2.8-folds higher in offspring born to EVOO compared with soybean oil-fed mothers. OPG, beta-catenin, and OPG/RANK-L expression were 88%, 94%, and 70% lower in offspring born to the 45% oil-fed mothers compared with the 16% group. In contrast, peroxisome proliferator-activated receptor gamma-2 (PPARγ2) gene expression was higher in the 45% oil group, adjusted for the types of oil. Conclusion: Maternal EVOO consumption, but not soybean oil increased osteoblastic gene expression, and high amounts of both oils decreased osteoblastic and increased adipogenic genes expression in adolescent offspring. |
topic |
Dietary oil Fetal programming Gestation Lactation Mouse Olive oil Osteoblastogenesis |
url |
http://ijbms.mums.ac.ir/article_7915_872136441a6d3030bdc67e9f1558aeaa.pdf |
work_keys_str_mv |
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