The predictive value of miR-155 expression in serum and tumor tissue of patients with colon cancer for tumor recurrence and the potential target genes

• Main Authors: Duan-Qin Diao, Kong-Hua Ren, Wen-Cai Jin, Rong Sun, Yun-Hua Chen
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-09-01
• Series: Journal of Hainan Medical University
• Subjects: Colon cancer; miR-155; Recurrence; Angiogenesis; Stem cell
• Online Access: http://www.hnykdxxb.com/PDF/201618/29.pdf

Objective: To study the predictive value of miR-155 expression in serum and tumor tissue of patients with colon cancer for tumor recurrence and the potential target genes. Methods: A total of 90 patients who received radical operation for colon cancer in our hospital from March 2010 to March 2011 were selected, followed up for 5 years and divided into recurrence group and non-recurrence group, plasma was collected before operation to determine the expression of miR-155, tumor tissue was collected after operation to determine the expression of miR- 155, stem cell marker molecules and angiogenesis marker molecules, and plasma was collected during follow-up to determine CEA, CA125 and CA199 levels. Results: miR-155 expression levels in plasma and tumor tissue of recurrence group were significantly higher than those of non-recurrence group; at the time of confirmed diagnosis of recurrence, plasma CEA, CA125 and CA199 levels of recurrence group were significantly higher than those of non-recurrence group; miR-155 expression levels in plasma and tumor tissue were positively correlated with CEA, CA125 and CA199 levels; the higher the miR-155 expression levels in tumor tissue, the higher the stem cell marker molecules CD133, Sox-2, Oct-4 and Lgr5 as well as angiogenesis marker molecules EGFR, VEGFR2, VEGFR3 and PDGFRA levels. Conclusion: High expression of miR-155 in serum and tumor tissue of patients with colon cancer is closely related to the tumor recurrence, the genes that miR-155 target and regulate include stem cellrelated genes and angiogenesis-related genes.