Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations
This study aimed to evaluate the combined use of the Concentrated Growth Factor (CGF) and the liquid phase of CGF (LPCGF) on dental implant surfaces, using a medical device to determine the migration of growth factors, from the implant surface to the recipient. The implants were permeated by autolog...
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doaj-9bbc6b9341f8463980ba6ca64759df8b2020-11-25T01:36:36ZengMDPI AGApplied Sciences2076-34172019-05-01910211410.3390/app9102114app9102114Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future ExpectationsAndrea Palermo0Franco Ferrante1Eleonora Stanca2Fabrizio Damiano3Antonio Gnoni4Tiziano Batani5Maria Annunziata Carluccio6Christian Demitri7Luisa Siculella8College of Medicine and Dentistry, Birmingham B4 6BN, UKIndependent Researcher, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyDepartment of Basic Medical Sciences, Neurosciences and Sense Organs, University of Bari “Aldo Moro”, 70124 Bari, ItalySilfradent srl, Via G. Di Vittorio n. 35/37, 47018 Santa Sofia (FC), ItalyNational Research Council, Institute of Clinical Physiology, 73100 Lecce, ItalyDepartment of Engineering for Innovation, University of Salento, 73100 Lecce, ItalyLaboratory of Molecular Biology, Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, ItalyThis study aimed to evaluate the combined use of the Concentrated Growth Factor (CGF) and the liquid phase of CGF (LPCGF) on dental implant surfaces, using a medical device to determine the migration of growth factors, from the implant surface to the recipient. The implants were permeated by autologous growth factors, using a specific centrifuge device. CGF adhesion on the implant surface was evaluated through a scanning electron microscope analysis. To assess the release of the vascular endothelial growth factor (VEGF) from CGF, LPCGF, and CGF- or LPCGF-permeated implant, an ELISA assay was carried out. The results showed that the concentration of the growth factor VEGF was greater in CGF than in LPCGF. Our innovative technique allowed the incorporation of autologous growth factors on the surface of the dental implants. Moreover, we reported the release of VEGF, over time, by CGF- or LPCGF-permeated implant. On this basis, it was possible to obtain a biologically active implant surface, essential to create intercellular communication and neo-angiogenesis, to facilitate wound healing and tissue regeneration.https://www.mdpi.com/2076-3417/9/10/2114bilateral osseointegrationgrowth factordental implant |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andrea Palermo Franco Ferrante Eleonora Stanca Fabrizio Damiano Antonio Gnoni Tiziano Batani Maria Annunziata Carluccio Christian Demitri Luisa Siculella |
spellingShingle |
Andrea Palermo Franco Ferrante Eleonora Stanca Fabrizio Damiano Antonio Gnoni Tiziano Batani Maria Annunziata Carluccio Christian Demitri Luisa Siculella Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations Applied Sciences bilateral osseointegration growth factor dental implant |
author_facet |
Andrea Palermo Franco Ferrante Eleonora Stanca Fabrizio Damiano Antonio Gnoni Tiziano Batani Maria Annunziata Carluccio Christian Demitri Luisa Siculella |
author_sort |
Andrea Palermo |
title |
Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations |
title_short |
Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations |
title_full |
Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations |
title_fullStr |
Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations |
title_full_unstemmed |
Release of VEGF from Dental Implant Surface (IML<sup>®</sup> Implant) Coated with Concentrated Growth Factors (CGF) and the Liquid Phase of CGF (LPCGF): In Vitro Results and Future Expectations |
title_sort |
release of vegf from dental implant surface (iml<sup>®</sup> implant) coated with concentrated growth factors (cgf) and the liquid phase of cgf (lpcgf): in vitro results and future expectations |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2019-05-01 |
description |
This study aimed to evaluate the combined use of the Concentrated Growth Factor (CGF) and the liquid phase of CGF (LPCGF) on dental implant surfaces, using a medical device to determine the migration of growth factors, from the implant surface to the recipient. The implants were permeated by autologous growth factors, using a specific centrifuge device. CGF adhesion on the implant surface was evaluated through a scanning electron microscope analysis. To assess the release of the vascular endothelial growth factor (VEGF) from CGF, LPCGF, and CGF- or LPCGF-permeated implant, an ELISA assay was carried out. The results showed that the concentration of the growth factor VEGF was greater in CGF than in LPCGF. Our innovative technique allowed the incorporation of autologous growth factors on the surface of the dental implants. Moreover, we reported the release of VEGF, over time, by CGF- or LPCGF-permeated implant. On this basis, it was possible to obtain a biologically active implant surface, essential to create intercellular communication and neo-angiogenesis, to facilitate wound healing and tissue regeneration. |
topic |
bilateral osseointegration growth factor dental implant |
url |
https://www.mdpi.com/2076-3417/9/10/2114 |
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