A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration
Several biomaterials have recently been developed to address the challenge of osteochondral regeneration. Among these, chitosan holds promises both for cartilage and bone healing. The aim of this in vivo study was to evaluate the regeneration potential of a novel hybrid magnesium-doped hydroxyapatit...
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doaj-9be17833e9854c8c9b4963e2675ad7892020-11-25T01:36:39ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-05-01209222710.3390/ijms20092227ijms20092227A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral RegenerationAlice Roffi0Elizaveta Kon1Francesco Perdisa2Milena Fini3Alessandro Di Martino4Annapaola Parrilli5Francesca Salamanna6Monica Sandri7Maria Sartori8Simone Sprio9Anna Tampieri10Maurilio Marcacci11Giuseppe Filardo12Applied and Translational Research (ATR) Center, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyKnee Joint Reconstruction Center—3rd Orthopedic Division, Humanitas Clinical Institute, 20089 Rozzano, ItalyHip and Knee Replacement Department, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyLaboratory of Preclinical and Surgical Studies, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyII Orthopedic and Traumatologic Clinic; IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyLaboratory of Preclinical and Surgical Studies, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyLaboratory of Preclinical and Surgical Studies, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyInstitute of Science and Technology for Ceramics, National Research Council (ISTEC-CNR), 48018 Faenza, ItalyLaboratory of Preclinical and Surgical Studies, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalyInstitute of Science and Technology for Ceramics, National Research Council (ISTEC-CNR), 48018 Faenza, ItalyInstitute of Science and Technology for Ceramics, National Research Council (ISTEC-CNR), 48018 Faenza, ItalyKnee Joint Reconstruction Center—3rd Orthopedic Division, Humanitas Clinical Institute, 20089 Rozzano, ItalyApplied and Translational Research (ATR) Center, IRCCS—Istituto Ortopedico Rizzoli, 40136 Bologna, ItalySeveral biomaterials have recently been developed to address the challenge of osteochondral regeneration. Among these, chitosan holds promises both for cartilage and bone healing. The aim of this in vivo study was to evaluate the regeneration potential of a novel hybrid magnesium-doped hydroxyapatite (MgHA), collagen, chitosan-based scaffold, which was tested in a sheep model to ascertain its osteochondral regenerative potential, and in a rabbit model to further evaluate its ability to regenerate bone tissue. Macroscopic, microtomography, histology, histomorphometry, and immunohistochemical analysis were performed. In the sheep model, all analyses did not show significant differences compared to untreated defects (<i>p</i> > 0.05), with no evidence of cartilage and subchondral bone regeneration. In the rabbit model, this bone scaffold provided less ability to enhance tissue healing compared with a commercial bone scaffold. Moreover, persistence of scaffold material and absence of integration with connective tissue around the scaffolds were observed. These results raised some concerns about the osteochondral use of this chitosan composite scaffold, especially for the bone layer. Further studies are needed to explore the best formulation of chitosan-reinforced composites for osteochondral treatment.https://www.mdpi.com/1422-0067/20/9/2227chitosanosteochondralbonecartilagefailurescaffold |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alice Roffi Elizaveta Kon Francesco Perdisa Milena Fini Alessandro Di Martino Annapaola Parrilli Francesca Salamanna Monica Sandri Maria Sartori Simone Sprio Anna Tampieri Maurilio Marcacci Giuseppe Filardo |
spellingShingle |
Alice Roffi Elizaveta Kon Francesco Perdisa Milena Fini Alessandro Di Martino Annapaola Parrilli Francesca Salamanna Monica Sandri Maria Sartori Simone Sprio Anna Tampieri Maurilio Marcacci Giuseppe Filardo A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration International Journal of Molecular Sciences chitosan osteochondral bone cartilage failure scaffold |
author_facet |
Alice Roffi Elizaveta Kon Francesco Perdisa Milena Fini Alessandro Di Martino Annapaola Parrilli Francesca Salamanna Monica Sandri Maria Sartori Simone Sprio Anna Tampieri Maurilio Marcacci Giuseppe Filardo |
author_sort |
Alice Roffi |
title |
A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration |
title_short |
A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration |
title_full |
A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration |
title_fullStr |
A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration |
title_full_unstemmed |
A Composite Chitosan-Reinforced Scaffold Fails to Provide Osteochondral Regeneration |
title_sort |
composite chitosan-reinforced scaffold fails to provide osteochondral regeneration |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2019-05-01 |
description |
Several biomaterials have recently been developed to address the challenge of osteochondral regeneration. Among these, chitosan holds promises both for cartilage and bone healing. The aim of this in vivo study was to evaluate the regeneration potential of a novel hybrid magnesium-doped hydroxyapatite (MgHA), collagen, chitosan-based scaffold, which was tested in a sheep model to ascertain its osteochondral regenerative potential, and in a rabbit model to further evaluate its ability to regenerate bone tissue. Macroscopic, microtomography, histology, histomorphometry, and immunohistochemical analysis were performed. In the sheep model, all analyses did not show significant differences compared to untreated defects (<i>p</i> > 0.05), with no evidence of cartilage and subchondral bone regeneration. In the rabbit model, this bone scaffold provided less ability to enhance tissue healing compared with a commercial bone scaffold. Moreover, persistence of scaffold material and absence of integration with connective tissue around the scaffolds were observed. These results raised some concerns about the osteochondral use of this chitosan composite scaffold, especially for the bone layer. Further studies are needed to explore the best formulation of chitosan-reinforced composites for osteochondral treatment. |
topic |
chitosan osteochondral bone cartilage failure scaffold |
url |
https://www.mdpi.com/1422-0067/20/9/2227 |
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