<it>In vitro</it> evaluation of <it>Pandanus amaryllifolius</it> ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis

• Main Authors: Chong Hueh, Yeap Swee, Rahmat Asmah, Akim Abdah, Alitheen Noorjahan, Othman Fauziah, Gwendoline-Ee Cheng
• Format: Article
• Language: English
• Published: BMC 2012-08-01
• Series: BMC Complementary and Alternative Medicine
• Subjects: <it>Pandanus amaryllifolius</it>; MDA-MB-231; Apoptosis; Cytochrome c; Caspase
• Online Access: http://www.biomedcentral.com/1472-6882/12/134

<p>Abstract</p> <p>Background</p> <p>Our previous study had shown that <it>P. amaryllifolius</it> was able to selectively inhibit cell proliferation of hormone independent breast cancer cell line MDA-MB-231. To understand the mode of killing and mechanism of action for <it>P. amaryllifolius</it>, the ethanol extract was evaluated for their alteration of cell cycle progression, PS externalization, DNA fragmentation and expression of anti/pro-apoptotic related protein.</p> <p>Results</p> <p>Cell cycle progression analysis, Annexin V and Tunel assays suggested that IC₅₀ of <it>P. amaryllifolius</it> ethanol extract induced G0/G1 cell cycle arrest, PS externalization and DNA fragmentation. On the other hand, ELISA for cytochrome c, caspase-3/7, 8 and 9 indicated that apoptosis was contributed by mitochondrial cytochrome c release via induction of caspase 3/7, 9, and p53 was associated with the suppression of XIAP in <it>P. amaryllifolius</it> treated MDA-MB-231 cells.</p> <p>Conclusion</p> <p>Our findings suggest that <it>P. amaryllifolius</it> ethanol extract induced apoptosis on hormone independent breast cancer cell line MDA-MB-231.</p>