Soft texture of atlantic salmon fillets is associated with glycogen accumulation.
Atlantic salmon (Salmo salar L.) with soft fillets are not suited for manufacturing high quality products. Therefore fillets with insufficient firmness are downgraded, leading to severe economic losses to the farming and processing industries. In the current study, morphological characteristics of s...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2014-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3887068?pdf=render |
id |
doaj-9bf980d186a94cdd834b228a3fe94c78 |
---|---|
record_format |
Article |
spelling |
doaj-9bf980d186a94cdd834b228a3fe94c782020-11-24T21:54:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8555110.1371/journal.pone.0085551Soft texture of atlantic salmon fillets is associated with glycogen accumulation.Jacob S TorgersenErling Olaf KoppangLars H StienAchim KohlerMona E PedersenTurid MørkøreAtlantic salmon (Salmo salar L.) with soft fillets are not suited for manufacturing high quality products. Therefore fillets with insufficient firmness are downgraded, leading to severe economic losses to the farming and processing industries. In the current study, morphological characteristics of salmon fillets ranging from soft to hard were analysed. Different microscopic techniques were applied, including novel methods in this field of research: morphometric image analysis, periodic acid Schiff staining, immunofluorescence microscopy, transmission electron microscopy and fourier transform infrared microscopy. The results showed that the myocytes of soft muscle had detached cells with mitochondrial dysfunctions, large glycogen aggregates and enlarged inter cellular areas, void of extracellular matrix proteins, including lower amounts of sulfated glycoproteins. Myofibre-myofibre detachment and disappearance of the endomysium in soft muscles coincided with deterioration of important connective tissue constituents such as Collagen type I (Col I), Perlecan and Aggrecan. In summary our investigations show for the first time an association between soft flesh of Atlantic salmon and massive intracellular glycogen accumulation coinciding with degenerated mitochondria, myocyte detachment and altered extracellular matrix protein distribution. The results are important for further understanding the etiology of soft salmon.http://europepmc.org/articles/PMC3887068?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jacob S Torgersen Erling Olaf Koppang Lars H Stien Achim Kohler Mona E Pedersen Turid Mørkøre |
spellingShingle |
Jacob S Torgersen Erling Olaf Koppang Lars H Stien Achim Kohler Mona E Pedersen Turid Mørkøre Soft texture of atlantic salmon fillets is associated with glycogen accumulation. PLoS ONE |
author_facet |
Jacob S Torgersen Erling Olaf Koppang Lars H Stien Achim Kohler Mona E Pedersen Turid Mørkøre |
author_sort |
Jacob S Torgersen |
title |
Soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
title_short |
Soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
title_full |
Soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
title_fullStr |
Soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
title_full_unstemmed |
Soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
title_sort |
soft texture of atlantic salmon fillets is associated with glycogen accumulation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Atlantic salmon (Salmo salar L.) with soft fillets are not suited for manufacturing high quality products. Therefore fillets with insufficient firmness are downgraded, leading to severe economic losses to the farming and processing industries. In the current study, morphological characteristics of salmon fillets ranging from soft to hard were analysed. Different microscopic techniques were applied, including novel methods in this field of research: morphometric image analysis, periodic acid Schiff staining, immunofluorescence microscopy, transmission electron microscopy and fourier transform infrared microscopy. The results showed that the myocytes of soft muscle had detached cells with mitochondrial dysfunctions, large glycogen aggregates and enlarged inter cellular areas, void of extracellular matrix proteins, including lower amounts of sulfated glycoproteins. Myofibre-myofibre detachment and disappearance of the endomysium in soft muscles coincided with deterioration of important connective tissue constituents such as Collagen type I (Col I), Perlecan and Aggrecan. In summary our investigations show for the first time an association between soft flesh of Atlantic salmon and massive intracellular glycogen accumulation coinciding with degenerated mitochondria, myocyte detachment and altered extracellular matrix protein distribution. The results are important for further understanding the etiology of soft salmon. |
url |
http://europepmc.org/articles/PMC3887068?pdf=render |
work_keys_str_mv |
AT jacobstorgersen softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation AT erlingolafkoppang softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation AT larshstien softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation AT achimkohler softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation AT monaepedersen softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation AT turidmørkøre softtextureofatlanticsalmonfilletsisassociatedwithglycogenaccumulation |
_version_ |
1725867630172045312 |