THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS

Safety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among sev...

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Main Authors: A. Bramante, F. Cecchi, E. Ciani, E. Castellana, M.S. D’Andrea, F. Pilla, R. Ciampolini
Format: Article
Language:English
Published: PAGEPress Publications 2011-04-01
Series:Italian Journal of Food Safety
Subjects:
Online Access:http://www.pagepressjournals.org/index.php/ijfs/article/view/749
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spelling doaj-9c17ee8c30e04a22a5907e0b476883d02020-11-25T03:50:51ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322011-04-0111414410.4081/ijfs.2011.1.41389THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTSA. Bramante0F. Cecchi1E. Ciani2E. Castellana3M.S. D’Andrea4F. Pilla5R. Ciampolini6Facoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiFacoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiFacoltà di Scienze Biotecnologiche dell’Università di Bari, Dipartimento di Fisiologia Generale ed AmbientaleFacoltà di Scienze Biotecnologiche dell’Università di Bari, Dipartimento di Fisiologia Generale ed AmbientaleFacoltà di Agraria, Università degli Studi del Molise, Dipartimento SAVAFacoltà di Agraria, Università degli Studi del Molise, Dipartimento SAVAFacoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiSafety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among seven Italian sheep breeds have been typed at 19 STR markers, together with 90 samples from both imported sheep animals and local crossbred animals withdrawn at slaughterhouses. A maximum likelihood assignment test was adopted to evaluate STR ability to allocate samples to their true breed of origin. Sarda animals were all correctly allocated, as well as more than 98% of samples from the other breeds. Only slightly worst allocation performances were observed for imported and crossbred animals. Preliminary results seem quite promising, though further analyses will be needed in order to better understand the statistical power of such an assignment test before implementation in the sheep meat production chain.http://www.pagepressjournals.org/index.php/ijfs/article/view/749Breed traceability, STR markers, probabilistic approach, likelihood, sheep products
collection DOAJ
language English
format Article
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author A. Bramante
F. Cecchi
E. Ciani
E. Castellana
M.S. D’Andrea
F. Pilla
R. Ciampolini
spellingShingle A. Bramante
F. Cecchi
E. Ciani
E. Castellana
M.S. D’Andrea
F. Pilla
R. Ciampolini
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
Italian Journal of Food Safety
Breed traceability, STR markers, probabilistic approach, likelihood, sheep products
author_facet A. Bramante
F. Cecchi
E. Ciani
E. Castellana
M.S. D’Andrea
F. Pilla
R. Ciampolini
author_sort A. Bramante
title THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
title_short THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
title_full THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
title_fullStr THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
title_full_unstemmed THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
title_sort breed traceability of sheep meat by using molecular genetics methods: preliminary results
publisher PAGEPress Publications
series Italian Journal of Food Safety
issn 2239-7132
publishDate 2011-04-01
description Safety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among seven Italian sheep breeds have been typed at 19 STR markers, together with 90 samples from both imported sheep animals and local crossbred animals withdrawn at slaughterhouses. A maximum likelihood assignment test was adopted to evaluate STR ability to allocate samples to their true breed of origin. Sarda animals were all correctly allocated, as well as more than 98% of samples from the other breeds. Only slightly worst allocation performances were observed for imported and crossbred animals. Preliminary results seem quite promising, though further analyses will be needed in order to better understand the statistical power of such an assignment test before implementation in the sheep meat production chain.
topic Breed traceability, STR markers, probabilistic approach, likelihood, sheep products
url http://www.pagepressjournals.org/index.php/ijfs/article/view/749
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