THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS
Safety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among sev...
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doaj-9c17ee8c30e04a22a5907e0b476883d02020-11-25T03:50:51ZengPAGEPress PublicationsItalian Journal of Food Safety2239-71322011-04-0111414410.4081/ijfs.2011.1.41389THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTSA. Bramante0F. Cecchi1E. Ciani2E. Castellana3M.S. D’Andrea4F. Pilla5R. Ciampolini6Facoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiFacoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiFacoltà di Scienze Biotecnologiche dell’Università di Bari, Dipartimento di Fisiologia Generale ed AmbientaleFacoltà di Scienze Biotecnologiche dell’Università di Bari, Dipartimento di Fisiologia Generale ed AmbientaleFacoltà di Agraria, Università degli Studi del Molise, Dipartimento SAVAFacoltà di Agraria, Università degli Studi del Molise, Dipartimento SAVAFacoltà di Medicina Veterinaria dell’Università di Pisa, Dipartimento di Patologia Animale, Profilassi e Igiene degli AlimentiSafety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among seven Italian sheep breeds have been typed at 19 STR markers, together with 90 samples from both imported sheep animals and local crossbred animals withdrawn at slaughterhouses. A maximum likelihood assignment test was adopted to evaluate STR ability to allocate samples to their true breed of origin. Sarda animals were all correctly allocated, as well as more than 98% of samples from the other breeds. Only slightly worst allocation performances were observed for imported and crossbred animals. Preliminary results seem quite promising, though further analyses will be needed in order to better understand the statistical power of such an assignment test before implementation in the sheep meat production chain.http://www.pagepressjournals.org/index.php/ijfs/article/view/749Breed traceability, STR markers, probabilistic approach, likelihood, sheep products |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
A. Bramante F. Cecchi E. Ciani E. Castellana M.S. D’Andrea F. Pilla R. Ciampolini |
spellingShingle |
A. Bramante F. Cecchi E. Ciani E. Castellana M.S. D’Andrea F. Pilla R. Ciampolini THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS Italian Journal of Food Safety Breed traceability, STR markers, probabilistic approach, likelihood, sheep products |
author_facet |
A. Bramante F. Cecchi E. Ciani E. Castellana M.S. D’Andrea F. Pilla R. Ciampolini |
author_sort |
A. Bramante |
title |
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS |
title_short |
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS |
title_full |
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS |
title_fullStr |
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS |
title_full_unstemmed |
THE BREED TRACEABILITY OF SHEEP MEAT BY USING MOLECULAR GENETICS METHODS: PRELIMINARY RESULTS |
title_sort |
breed traceability of sheep meat by using molecular genetics methods: preliminary results |
publisher |
PAGEPress Publications |
series |
Italian Journal of Food Safety |
issn |
2239-7132 |
publishDate |
2011-04-01 |
description |
Safety and quality foods of animal origin are extremely important for consumers. The aim of this work was to evaluate the feasibility of a method to track the breed origin of sheep meat all along the production chain using molecular genetics tools. A total of 800 samples evenly distributed among seven Italian sheep breeds have been typed at 19 STR markers, together with 90 samples from both imported sheep animals and local crossbred animals withdrawn at slaughterhouses. A maximum likelihood assignment test was adopted to evaluate STR ability to allocate samples to their true breed of origin. Sarda animals were all correctly allocated, as well as more than 98% of samples from the other breeds. Only slightly worst allocation performances were observed for imported and crossbred animals. Preliminary results seem quite promising, though further analyses will be needed in order to better understand the statistical power of such an assignment test before implementation in the sheep meat production chain. |
topic |
Breed traceability, STR markers, probabilistic approach, likelihood, sheep products |
url |
http://www.pagepressjournals.org/index.php/ijfs/article/view/749 |
work_keys_str_mv |
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