The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy
Abstract.: It has been known that oxidative stress plays an important role in the development of diabetic nephropathy (DN). The antioxidative effects of Astragalus saponin I (AS I) were studied in vitro and in vivo. In the presence of high glucose and H2O2, the total antioxidative capability, catala...
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doaj-9c196b4fc3844b68821b9df157becc472020-11-24T21:49:18ZengElsevierJournal of Pharmacological Sciences1347-86132006-01-011012166173The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic NephropathyYin Xiaoxing0Zhang Yindi1Yu Junxian2Zhang Pei3Shen Jianping4Qiu Jun5Wu Haiwei6Zhu Xiang7Departments of Pharmacology, Nanjing Medical University, Nanjing, 210029, ChinaDepartments of Pharmacology, Nanjing Medical University, Nanjing, 210029, China; Corresponding author. ydzhang@njmu.edu.cnDepartments of Pharmacology, Nanjing Medical University, Nanjing, 210029, ChinaDepartments of Pharmacology, Nanjing Medical University, Nanjing, 210029, ChinaDepartments of Pharmacology, Nanjing Medical University, Nanjing, 210029, ChinaDepartments of Pharmacology, Nanjing Medical University, Nanjing, 210029, ChinaPathogenic Biology, Nanjing Medical University, Nanjing, 210029, ChinaPathogenic Biology, Nanjing Medical University, Nanjing, 210029, ChinaAbstract.: It has been known that oxidative stress plays an important role in the development of diabetic nephropathy (DN). The antioxidative effects of Astragalus saponin I (AS I) were studied in vitro and in vivo. In the presence of high glucose and H2O2, the total antioxidative capability, catalase, reduced glutathione, and superoxide dismutase level of rat mesangial cells were significantly decreased, and transforming growth factor β1 (TGF-β1) mRNA level, collagen IV, and laminin level were significantly increased. When compared with those in the high glucose group, these 4 indexes of cells incubated in 2.0 and/or 20 µmol/L of AS I were significantly enhanced, and levels of TGF-β1 mRNA, collagen IV and laminin were statistically decreased. By flowcytomery, percentages of S phase of cells incubated in high glucose and H2O2 were lowered, while those in AS I were increased. Furthermore, the physical behaviors of rats treated with 12 mg/kg of AS I restored with vigor and weight gaining, while the level of HbAlC was significantly reduced. Thus, AS I has antioxidative effects and is a potential compound worth further study because it may prevent the development of DN. Keywords:: Astragalus saponin I, diabetic nephropathy, antioxidative, mesangial cellhttp://www.sciencedirect.com/science/article/pii/S1347861319344627 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yin Xiaoxing Zhang Yindi Yu Junxian Zhang Pei Shen Jianping Qiu Jun Wu Haiwei Zhu Xiang |
spellingShingle |
Yin Xiaoxing Zhang Yindi Yu Junxian Zhang Pei Shen Jianping Qiu Jun Wu Haiwei Zhu Xiang The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy Journal of Pharmacological Sciences |
author_facet |
Yin Xiaoxing Zhang Yindi Yu Junxian Zhang Pei Shen Jianping Qiu Jun Wu Haiwei Zhu Xiang |
author_sort |
Yin Xiaoxing |
title |
The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy |
title_short |
The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy |
title_full |
The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy |
title_fullStr |
The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy |
title_full_unstemmed |
The Antioxidative Effects of Astragalus Saponin I Protect Against Development of Early Diabetic Nephropathy |
title_sort |
antioxidative effects of astragalus saponin i protect against development of early diabetic nephropathy |
publisher |
Elsevier |
series |
Journal of Pharmacological Sciences |
issn |
1347-8613 |
publishDate |
2006-01-01 |
description |
Abstract.: It has been known that oxidative stress plays an important role in the development of diabetic nephropathy (DN). The antioxidative effects of Astragalus saponin I (AS I) were studied in vitro and in vivo. In the presence of high glucose and H2O2, the total antioxidative capability, catalase, reduced glutathione, and superoxide dismutase level of rat mesangial cells were significantly decreased, and transforming growth factor β1 (TGF-β1) mRNA level, collagen IV, and laminin level were significantly increased. When compared with those in the high glucose group, these 4 indexes of cells incubated in 2.0 and/or 20 µmol/L of AS I were significantly enhanced, and levels of TGF-β1 mRNA, collagen IV and laminin were statistically decreased. By flowcytomery, percentages of S phase of cells incubated in high glucose and H2O2 were lowered, while those in AS I were increased. Furthermore, the physical behaviors of rats treated with 12 mg/kg of AS I restored with vigor and weight gaining, while the level of HbAlC was significantly reduced. Thus, AS I has antioxidative effects and is a potential compound worth further study because it may prevent the development of DN. Keywords:: Astragalus saponin I, diabetic nephropathy, antioxidative, mesangial cell |
url |
http://www.sciencedirect.com/science/article/pii/S1347861319344627 |
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