Kobuviruses carried by Rattus norvegicus in Guangdong, China
Abstract Background Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvir...
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doaj-9c1bc8b9908c491bb0efe83dff8a57012020-11-25T01:43:53ZengBMCBMC Microbiology1471-21802020-04-012011810.1186/s12866-020-01767-xKobuviruses carried by Rattus norvegicus in Guangdong, ChinaFang-Fei You0Min-Yi Zhang1Huan He2Wen-Qiao He3Yong-Zhi Li4Qing Chen5Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityDepartment of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical UniversityAbstract Background Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China. Results Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%. Conclusion Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage.http://link.springer.com/article/10.1186/s12866-020-01767-xKobuvirusRattus norvegicusPrevalenceGenetic characteristic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fang-Fei You Min-Yi Zhang Huan He Wen-Qiao He Yong-Zhi Li Qing Chen |
spellingShingle |
Fang-Fei You Min-Yi Zhang Huan He Wen-Qiao He Yong-Zhi Li Qing Chen Kobuviruses carried by Rattus norvegicus in Guangdong, China BMC Microbiology Kobuvirus Rattus norvegicus Prevalence Genetic characteristic |
author_facet |
Fang-Fei You Min-Yi Zhang Huan He Wen-Qiao He Yong-Zhi Li Qing Chen |
author_sort |
Fang-Fei You |
title |
Kobuviruses carried by Rattus norvegicus in Guangdong, China |
title_short |
Kobuviruses carried by Rattus norvegicus in Guangdong, China |
title_full |
Kobuviruses carried by Rattus norvegicus in Guangdong, China |
title_fullStr |
Kobuviruses carried by Rattus norvegicus in Guangdong, China |
title_full_unstemmed |
Kobuviruses carried by Rattus norvegicus in Guangdong, China |
title_sort |
kobuviruses carried by rattus norvegicus in guangdong, china |
publisher |
BMC |
series |
BMC Microbiology |
issn |
1471-2180 |
publishDate |
2020-04-01 |
description |
Abstract Background Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China. Results Fecal samples from 223 rats (Rattus norvegicus) were collected from Guangdong and kobuviruses were detected in 12.6% (28) of samples. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions showed that rat kobuvirus obtained in this study were genetically closely related to those of rat/mouse kobuvirus reported in other geographical areas. Two near full-length rat kobuvirus genomes (MM33, GZ85) were acquired and phylogenetic analysis of these revealed that they shared very high nucleotide/amino acids identity with one another (95.4%/99.4%) and a sewage-derived sequence (86.9%/93.5% and 87.5%/93.7%, respectively). Comparison with original Aichivirus A strains, such human kobuvirus, revealed amino acid identity values of approximately 80%. Conclusion Our findings indicate that rat kobuvirus have distinctive genetic characteristics from other Aichivirus A viruses. Additionally, rat kobuvirus may spread via sewage. |
topic |
Kobuvirus Rattus norvegicus Prevalence Genetic characteristic |
url |
http://link.springer.com/article/10.1186/s12866-020-01767-x |
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