Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus
V2 proteins encoded by some whitefly-transmitted geminiviruses were reported to be functionally important proteins. However, the functions of the V2 protein of tobacco curly shoot virus (TbCSV), a monopartite begomovirus that causes leaf curl disease on tomato and tobacco in China, remains to be cha...
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doaj-9c1da5775719437da851603a224d4be12021-01-19T00:04:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-012292392310.3390/ijms22020923Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot VirusMingjun Li *0Changchang Li1Kairong Jiang2Ke Li3Junlei Zhang4Miao Sun5Gentu Wu6Ling Qing7Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaChongqing Key Laboratory of Plant Disease Biology, College of Plant Protection, Southwest University, Chongqing 400716, ChinaV2 proteins encoded by some whitefly-transmitted geminiviruses were reported to be functionally important proteins. However, the functions of the V2 protein of tobacco curly shoot virus (TbCSV), a monopartite begomovirus that causes leaf curl disease on tomato and tobacco in China, remains to be characterized. In our report, an <i>Agrobacterium</i> infiltration-mediated transient expression assay indicated that TbCSV V2 can suppress local and systemic RNA silencing and the deletion analyses demonstrated that the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression. Site-directed substitutions showed that the conserved basic and ring-structured amino acids in TbCSV V2 are critical for its suppressor activity. Potato virus X-mediated heteroexpression of TbCSV V2 in <i>N</i>icotiana<i> </i><i>benthamiana</i> induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity. Furthermore, TbCSV infectious clone mutant with untranslated V2 protein (TbCSV<sub>∆</sub><sub>V2</sub>) could not induce visual symptoms, and coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation and symptom development. Interestingly, symptom recovery occurred at 15 days postinoculation (dpi) and onward in TbCSV<sub>∆</sub><sub>V2</sub>/TbCSB-inoculated plants. The presented work contributes to understanding the RNA silencing suppression activity of TbCSV V2 and extends our knowledge of the multifunctional role of begomovirus-encoded V2 proteins during viral infections.https://www.mdpi.com/1422-0067/22/2/923tobacco curly shoot virusV2RNA silencing suppressorpathogenicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mingjun Li * Changchang Li Kairong Jiang Ke Li Junlei Zhang Miao Sun Gentu Wu Ling Qing |
spellingShingle |
Mingjun Li * Changchang Li Kairong Jiang Ke Li Junlei Zhang Miao Sun Gentu Wu Ling Qing Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus International Journal of Molecular Sciences tobacco curly shoot virus V2 RNA silencing suppressor pathogenicity |
author_facet |
Mingjun Li * Changchang Li Kairong Jiang Ke Li Junlei Zhang Miao Sun Gentu Wu Ling Qing |
author_sort |
Mingjun Li * |
title |
Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus |
title_short |
Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus |
title_full |
Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus |
title_fullStr |
Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus |
title_full_unstemmed |
Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus |
title_sort |
characterization of pathogenicity-associated v2 protein of tobacco curly shoot virus |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-01-01 |
description |
V2 proteins encoded by some whitefly-transmitted geminiviruses were reported to be functionally important proteins. However, the functions of the V2 protein of tobacco curly shoot virus (TbCSV), a monopartite begomovirus that causes leaf curl disease on tomato and tobacco in China, remains to be characterized. In our report, an <i>Agrobacterium</i> infiltration-mediated transient expression assay indicated that TbCSV V2 can suppress local and systemic RNA silencing and the deletion analyses demonstrated that the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression. Site-directed substitutions showed that the conserved basic and ring-structured amino acids in TbCSV V2 are critical for its suppressor activity. Potato virus X-mediated heteroexpression of TbCSV V2 in <i>N</i>icotiana<i> </i><i>benthamiana</i> induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity. Furthermore, TbCSV infectious clone mutant with untranslated V2 protein (TbCSV<sub>∆</sub><sub>V2</sub>) could not induce visual symptoms, and coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation and symptom development. Interestingly, symptom recovery occurred at 15 days postinoculation (dpi) and onward in TbCSV<sub>∆</sub><sub>V2</sub>/TbCSB-inoculated plants. The presented work contributes to understanding the RNA silencing suppression activity of TbCSV V2 and extends our knowledge of the multifunctional role of begomovirus-encoded V2 proteins during viral infections. |
topic |
tobacco curly shoot virus V2 RNA silencing suppressor pathogenicity |
url |
https://www.mdpi.com/1422-0067/22/2/923 |
work_keys_str_mv |
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