Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i>
<b>Background:</b> <i>Angelica sinensis</i> is a medicinal plant known for a variety of biological effects, including its ability to stimulate innate immune cells in humans. Recent studies indicate that the immunostimulatory activity of <i>A. sinensis</i> arises f...
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doaj-9c4c0c4798b84fe59d4880b4652b2a482020-11-25T02:28:28ZengMDPI AGMedicines2305-63202019-05-01626210.3390/medicines6020062medicines6020062Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i>Kriti Kalpana0Diego Montenegro1Giovanna Romero2Ximena Peralta3Betul Akgol Oksuz4Adriana Heguy5Moriya Tsuji6Akira Kawamura7Department of Chemistry, Hunter College of CUNY, New York, NY 10065, USADepartment of Chemistry, Hunter College of CUNY, New York, NY 10065, USADepartment of Chemistry, Hunter College of CUNY, New York, NY 10065, USADepartment of Chemistry, Hunter College of CUNY, New York, NY 10065, USAGenome Technology Center, NYU Langone Medical Center, New York, NY 10016, USAGenome Technology Center, NYU Langone Medical Center, New York, NY 10016, USAHIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, Affiliate of The Rockefeller University, New York, NY 10016, USADepartment of Chemistry, Hunter College of CUNY, New York, NY 10065, USA<b>Background:</b> <i>Angelica sinensis</i> is a medicinal plant known for a variety of biological effects, including its ability to stimulate innate immune cells in humans. Recent studies indicate that the immunostimulatory activity of <i>A. sinensis</i> arises from microbe-associated molecular patterns (MAMPs) of plant-associated bacteria. However, it is unknown which bacterial taxa in <i>A. sinensis</i> are responsible for the production of immunostimulatory MAMPs. <b>Methods:</b> Samples of <i>A. sinensis</i> were subjected to a cell-based assay to detect monocyte-stimulation and 16S ribosomal RNA amplicon sequencing, which revealed their immunostimulatory activity and microbial communities. The resulting data were analyzed by Linear discriminant analysis effect size (LEfSe), an online biostatistical tool for metagenomic biomarker discovery, to identify the bacterial taxonomical features correlated with the immunostimulatory activity. <b>Results:</b> A series of bacterial taxa under <i>Gammaproteobacteria</i> correlated positively with the immunostimulatory activity, whereas several Gram-positive taxa and <i>Betaproteobacteria</i> correlated negatively with the activity. <b>Conclusions:</b> The identified bacterial taxa set a new stage to characterize immunostimulatory MAMPs in plants.https://www.mdpi.com/2305-6320/6/2/62microbe-associated molecular pattern (MAMP)lipopolysaccharide (LPS)<i>Angelica sinensis</i>Dong quaiShi-Quan-Da-Bu-TangJuzen-taiho-to |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Kriti Kalpana Diego Montenegro Giovanna Romero Ximena Peralta Betul Akgol Oksuz Adriana Heguy Moriya Tsuji Akira Kawamura |
spellingShingle |
Kriti Kalpana Diego Montenegro Giovanna Romero Ximena Peralta Betul Akgol Oksuz Adriana Heguy Moriya Tsuji Akira Kawamura Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> Medicines microbe-associated molecular pattern (MAMP) lipopolysaccharide (LPS) <i>Angelica sinensis</i> Dong quai Shi-Quan-Da-Bu-Tang Juzen-taiho-to |
author_facet |
Kriti Kalpana Diego Montenegro Giovanna Romero Ximena Peralta Betul Akgol Oksuz Adriana Heguy Moriya Tsuji Akira Kawamura |
author_sort |
Kriti Kalpana |
title |
Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> |
title_short |
Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> |
title_full |
Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> |
title_fullStr |
Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> |
title_full_unstemmed |
Abundance of Plant-Associated <i>Gammaproteobacteria</i> Correlates with Immunostimulatory Activity of <i>Angelica sinensis</i> |
title_sort |
abundance of plant-associated <i>gammaproteobacteria</i> correlates with immunostimulatory activity of <i>angelica sinensis</i> |
publisher |
MDPI AG |
series |
Medicines |
issn |
2305-6320 |
publishDate |
2019-05-01 |
description |
<b>Background:</b> <i>Angelica sinensis</i> is a medicinal plant known for a variety of biological effects, including its ability to stimulate innate immune cells in humans. Recent studies indicate that the immunostimulatory activity of <i>A. sinensis</i> arises from microbe-associated molecular patterns (MAMPs) of plant-associated bacteria. However, it is unknown which bacterial taxa in <i>A. sinensis</i> are responsible for the production of immunostimulatory MAMPs. <b>Methods:</b> Samples of <i>A. sinensis</i> were subjected to a cell-based assay to detect monocyte-stimulation and 16S ribosomal RNA amplicon sequencing, which revealed their immunostimulatory activity and microbial communities. The resulting data were analyzed by Linear discriminant analysis effect size (LEfSe), an online biostatistical tool for metagenomic biomarker discovery, to identify the bacterial taxonomical features correlated with the immunostimulatory activity. <b>Results:</b> A series of bacterial taxa under <i>Gammaproteobacteria</i> correlated positively with the immunostimulatory activity, whereas several Gram-positive taxa and <i>Betaproteobacteria</i> correlated negatively with the activity. <b>Conclusions:</b> The identified bacterial taxa set a new stage to characterize immunostimulatory MAMPs in plants. |
topic |
microbe-associated molecular pattern (MAMP) lipopolysaccharide (LPS) <i>Angelica sinensis</i> Dong quai Shi-Quan-Da-Bu-Tang Juzen-taiho-to |
url |
https://www.mdpi.com/2305-6320/6/2/62 |
work_keys_str_mv |
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