The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria
The aims of liophilization (freeze-drying) of lactic acid bacteria are to preserve pure cultures or to prepare starters for the dairy industry. In both cases, the choice of the cryoprotectant is very important. In this work, samples of Bifidobacterium breve A71 and Bifidobacterium bifidum B...
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Serbian Chemical Society
2001-01-01
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doaj-9c6b719f66c14b61a3a6a2bff8f198ff2020-12-24T07:35:25ZengSerbian Chemical Society Journal of the Serbian Chemical Society0352-51391820-74212001-01-0166743544110.2298/JSC0107435T0352-51390107435TThe influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteriaTršić-Milanović Nada0Kodžić Aleksandar1Baras Josip2Dimitrijević-Branković Suzana3Institute of Immunology and Virology “Torlak”, BelgradeInstitute of Immunology and Virology “Torlak”, BelgradeFaculty of Technology and Metallurgy, BelgradeFaculty of Technology and Metallurgy, BelgradeThe aims of liophilization (freeze-drying) of lactic acid bacteria are to preserve pure cultures or to prepare starters for the dairy industry. In both cases, the choice of the cryoprotectant is very important. In this work, samples of Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD were freeze-dried in a new cryoprotective medium containing lactose, gelatine and glycerol (medium B). The reference medium contained saccharose, gelatine and skim milk (medium A). Before liophilization, the eutectic points of both media were determined, because the products must be cooled to a temperature below its freezing point. The success of the cryoprotectants was estimated in terms of the number of surviving organisms after lyophilization. Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD freeze-dried in mediaAandBshowed high survival rates. Bifidobacterium breve A71 showed a greater percentage survival in combination with medium B than with medium A. These results could be utilized in the manufacture of Bifidobacterium breve A71 as a starter in the diary industry because it is a human isolate which, except for acidification, has probiotic activity.http://www.doiserbia.nb.rs/img/doi/0352-5139/2001/0352-51390107435T.pdflyophilizationlactic acid bacteriabifidobacterium bifidumcryoprotectants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tršić-Milanović Nada Kodžić Aleksandar Baras Josip Dimitrijević-Branković Suzana |
spellingShingle |
Tršić-Milanović Nada Kodžić Aleksandar Baras Josip Dimitrijević-Branković Suzana The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria Journal of the Serbian Chemical Society lyophilization lactic acid bacteria bifidobacterium bifidum cryoprotectants |
author_facet |
Tršić-Milanović Nada Kodžić Aleksandar Baras Josip Dimitrijević-Branković Suzana |
author_sort |
Tršić-Milanović Nada |
title |
The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
title_short |
The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
title_full |
The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
title_fullStr |
The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
title_full_unstemmed |
The influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
title_sort |
influence of a cryoprotective medium containing glycerol on the lyophilization of lactic acid bacteria |
publisher |
Serbian Chemical Society |
series |
Journal of the Serbian Chemical Society |
issn |
0352-5139 1820-7421 |
publishDate |
2001-01-01 |
description |
The aims of liophilization (freeze-drying) of lactic acid bacteria are to
preserve pure cultures or to prepare starters for the dairy industry. In
both cases, the choice of the cryoprotectant is very important. In this
work, samples of Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD
were freeze-dried in a new cryoprotective medium containing lactose,
gelatine and glycerol (medium B). The reference medium contained saccharose,
gelatine and skim milk (medium A). Before liophilization, the eutectic
points of both media were determined, because the products must be cooled to
a temperature below its freezing point. The success of the cryoprotectants
was estimated in terms of the number of surviving organisms after
lyophilization. Bifidobacterium breve A71 and Bifidobacterium bifidum BbTD
freeze-dried in mediaAandBshowed high survival rates. Bifidobacterium breve
A71 showed a greater percentage survival in combination with medium B than
with medium A. These results could be utilized in the manufacture of
Bifidobacterium breve A71 as a starter in the diary industry because it is a
human isolate which, except for acidification, has probiotic activity. |
topic |
lyophilization lactic acid bacteria bifidobacterium bifidum cryoprotectants |
url |
http://www.doiserbia.nb.rs/img/doi/0352-5139/2001/0352-51390107435T.pdf |
work_keys_str_mv |
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