Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives

Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives

Here we provide data on accessibility of nucleolus-like bodies (NLBs) of fully-grown (GV) mouse oocytes to fluorescence in situ hybridization (FISH) probes and anti-nucleolar antibodies as well as on oocyte general morphology and large scale chromatin configuration, which relate to the research arti...

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Main Authors: Kseniya V. Shishova, Yuriy M. Khodarovich, Elena A. Lavrentyeva, Olga V. Zatsepina
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340916301937
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spelling doaj-9c6f2c6c6e8c4d289ecde48bbca530e22020-11-25T02:04:15ZengElsevierData in Brief2352-34092016-06-01711791184Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixativesKseniya V. Shishova0Yuriy M. Khodarovich1Elena A. Lavrentyeva2Olga V. Zatsepina3Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian FederationShemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian FederationShemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation; Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, GSP-1, Leninskiye Gory, MSU, 1-73, Office 433, Moscow 119991, Russian FederationShemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation; Corresponding author. Tel.: +7 495 779 23 66.Here we provide data on accessibility of nucleolus-like bodies (NLBs) of fully-grown (GV) mouse oocytes to fluorescence in situ hybridization (FISH) probes and anti-nucleolar antibodies as well as on oocyte general morphology and large scale chromatin configuration, which relate to the research article “High-resolution microscopy of active ribosomal genes and key members of the rRNA processing machinery inside nucleolus-like bodies of fully-grown mouse oocytes” (Shishova et al., 2015 [1]). Experimental factors include: a cross-linking reagent formaldehyde and two denaturing fixatives, such as 70% ethanol and a mixture of absolute methanol and glacial acetic acid (3:1, v/v). Keywords: GV oocytes, Nucleolus-like bodies, Fixation procedure, RNA, Proteinshttp://www.sciencedirect.com/science/article/pii/S2352340916301937
collection DOAJ
language English
format Article
sources DOAJ
author Kseniya V. Shishova
Yuriy M. Khodarovich
Elena A. Lavrentyeva
Olga V. Zatsepina
spellingShingle Kseniya V. Shishova
Yuriy M. Khodarovich
Elena A. Lavrentyeva
Olga V. Zatsepina
Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
Data in Brief
author_facet Kseniya V. Shishova
Yuriy M. Khodarovich
Elena A. Lavrentyeva
Olga V. Zatsepina
author_sort Kseniya V. Shishova
title Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
title_short Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
title_full Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
title_fullStr Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
title_full_unstemmed Data on morphology, large-scale chromatin configuration and the occurrence of proteins and rRNA in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
title_sort data on morphology, large-scale chromatin configuration and the occurrence of proteins and rrna in nucleolus-like bodies of fully-grown mouse oocytes in different fixatives
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2016-06-01
description Here we provide data on accessibility of nucleolus-like bodies (NLBs) of fully-grown (GV) mouse oocytes to fluorescence in situ hybridization (FISH) probes and anti-nucleolar antibodies as well as on oocyte general morphology and large scale chromatin configuration, which relate to the research article “High-resolution microscopy of active ribosomal genes and key members of the rRNA processing machinery inside nucleolus-like bodies of fully-grown mouse oocytes” (Shishova et al., 2015 [1]). Experimental factors include: a cross-linking reagent formaldehyde and two denaturing fixatives, such as 70% ethanol and a mixture of absolute methanol and glacial acetic acid (3:1, v/v). Keywords: GV oocytes, Nucleolus-like bodies, Fixation procedure, RNA, Proteins
url http://www.sciencedirect.com/science/article/pii/S2352340916301937
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