Identification of Carotenoids in Halimeda macroloba Reef Associated Bacteria

Several carotenoid pigments which are produced by sea microorganisms are shown to function as antimicrobe compounds and potential antioxidants. Coral reefs with high levels of diverse biota facilitate Halimeda as a primary component and producer to have associated bacteria that produce relatively un...

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Bibliographic Details
Main Authors: Wiwik Astuti, Ocky Karna Radjasa, Ferry Fredy Karwur, Ferdy S. Rondonuwu
Format: Article
Language:English
Published: Diponegoro University; Association of Indonesian Coastal Management Experts 2016-12-01
Series:Ilmu Kelautan
Online Access:http://ejournal.undip.ac.id/index.php/ijms/article/view/10523
Description
Summary:Several carotenoid pigments which are produced by sea microorganisms are shown to function as antimicrobe compounds and potential antioxidants. Coral reefs with high levels of diverse biota facilitate Halimeda as a primary component and producer to have associated bacteria that produce relatively unknown metabolic compounds. This research attempts to isolate bacteria that are associated with Halimeda macroloba, identify it, as well as analyze the pigment content produced. A yellow-orange bacteria colony is successfully isolated and given the temporary name MK_HM. This bacteria is in the shape of circular (oval) rods and are gram-variable bacteria. Based on the sequencing analytical results, Blast homology, as well as a phylogenetic analysis, it shows that the bacteria have a relationship with Exiguobacterium aestuarii TF-16 at 94%, so that it can be strongly predicted that the MK_HM strain is a new species. This pigment analysis, which is conducted on the Exiguobacterium sp. MK_HM bacteria extract, reveals that this strain produces carotenoid pigments of diadinochrome, semi-α-carotenone, dinoxanthin, and P457.   Keywords: Exiguobacterium, carotenoid, diadinochrome, semi-α-carotenone, dinoxanthin, and P457.
ISSN:0853-7291
2406-7598