Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings
Melatonin (N-acetyl-5-methoxytryptamine) plays important role in multiple plant developmental processes and stress responses. We investigated the possible mediatory role of melatonin in growth, photosynthesis, and the response to cold stress in rice by using three different experiments: soaking seed...
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doaj-9c7ea4fe1e64482488fe7d8c711ed9672020-11-24T22:43:56ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2017-05-01810.3389/fpls.2017.00785250226Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice SeedlingsQiao-Hong Han0Bo Huang1Chun-Bang Ding2Zhong-Wei Zhang3Yang-Er Chen4Chao Hu5Li-Jun Zhou6Yan Huang7Jin-Qiu Liao8Shu Yuan9Ming Yuan10College of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Resources, Sichuan Agricultural UniversityChengdu, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaCollege of Resources, Sichuan Agricultural UniversityChengdu, ChinaCollege of Life Sciences, Sichuan Agricultural UniversityYa’an, ChinaMelatonin (N-acetyl-5-methoxytryptamine) plays important role in multiple plant developmental processes and stress responses. We investigated the possible mediatory role of melatonin in growth, photosynthesis, and the response to cold stress in rice by using three different experiments: soaking seed; immersing roots, and spraying to leaves with 0, 20, or 100 μM melatonin. After 6 days of cold stress, the growth of rice seedlings was significantly inhibited, but this inhibition was alleviated by exogenous melatonin. Furthermore, exogenous melatonin pretreatment alleviated the accumulation of reactive oxygen species, malondialdehyde and cell death induced by cold stress. Melatonin pretreatment also relieved the stress-induced inhibitions to photosynthesis and photosystem II activities. Further investigations showed that, antioxidant enzyme activities and non-enzymatic antioxidant levels were increased by melatonin pretreatments. The treatment methods of seed soaking and root immersion were more effective in improving cold stress resistance than the spraying method. The results also indicated the dose-dependent response of melatonin on rice physiological, biochemical, and photosynthetic parameters.http://journal.frontiersin.org/article/10.3389/fpls.2017.00785/fullmelatonincold stressreactive oxygen speciesphotosynthetic parameterschlorophyll fluorescence |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qiao-Hong Han Bo Huang Chun-Bang Ding Zhong-Wei Zhang Yang-Er Chen Chao Hu Li-Jun Zhou Yan Huang Jin-Qiu Liao Shu Yuan Ming Yuan |
spellingShingle |
Qiao-Hong Han Bo Huang Chun-Bang Ding Zhong-Wei Zhang Yang-Er Chen Chao Hu Li-Jun Zhou Yan Huang Jin-Qiu Liao Shu Yuan Ming Yuan Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings Frontiers in Plant Science melatonin cold stress reactive oxygen species photosynthetic parameters chlorophyll fluorescence |
author_facet |
Qiao-Hong Han Bo Huang Chun-Bang Ding Zhong-Wei Zhang Yang-Er Chen Chao Hu Li-Jun Zhou Yan Huang Jin-Qiu Liao Shu Yuan Ming Yuan |
author_sort |
Qiao-Hong Han |
title |
Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings |
title_short |
Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings |
title_full |
Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings |
title_fullStr |
Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings |
title_full_unstemmed |
Effects of Melatonin on Anti-oxidative Systems and Photosystem II in Cold-Stressed Rice Seedlings |
title_sort |
effects of melatonin on anti-oxidative systems and photosystem ii in cold-stressed rice seedlings |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2017-05-01 |
description |
Melatonin (N-acetyl-5-methoxytryptamine) plays important role in multiple plant developmental processes and stress responses. We investigated the possible mediatory role of melatonin in growth, photosynthesis, and the response to cold stress in rice by using three different experiments: soaking seed; immersing roots, and spraying to leaves with 0, 20, or 100 μM melatonin. After 6 days of cold stress, the growth of rice seedlings was significantly inhibited, but this inhibition was alleviated by exogenous melatonin. Furthermore, exogenous melatonin pretreatment alleviated the accumulation of reactive oxygen species, malondialdehyde and cell death induced by cold stress. Melatonin pretreatment also relieved the stress-induced inhibitions to photosynthesis and photosystem II activities. Further investigations showed that, antioxidant enzyme activities and non-enzymatic antioxidant levels were increased by melatonin pretreatments. The treatment methods of seed soaking and root immersion were more effective in improving cold stress resistance than the spraying method. The results also indicated the dose-dependent response of melatonin on rice physiological, biochemical, and photosynthetic parameters. |
topic |
melatonin cold stress reactive oxygen species photosynthetic parameters chlorophyll fluorescence |
url |
http://journal.frontiersin.org/article/10.3389/fpls.2017.00785/full |
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