A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease
Hand, foot, and mouth disease (HFMD) is a common viral illness affecting infants and children that is usually caused by Coxsackievirus A16 (CVA-16). To diagnose HFMD, we developed a method for rapid detection of CVA-16 based on reverse transcription-polymerase spiral reaction (RT-PSR). We used two p...
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doaj-9c8372b7b74548a6a1321aae9185833c2020-11-25T02:56:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00734528917A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth DiseaseShiyu He0Yanzhi Huang1Yanling Zhao2Bo Pang3Lixue Wang4Liwei Sun5Haoyan Yu6Juan Wang7Juan Li8Xiuling Song9Hui Li10Department of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaResearch Laboratory, Changchun Children’s Hospital, Changchun, ChinaResearch Laboratory, Changchun Children’s Hospital, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaResearch Laboratory, Changchun Children’s Hospital, Changchun, ChinaResearch Laboratory, Changchun Children’s Hospital, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaDepartment of Hygienic Inspection, School of Public Health, Jilin University, Changchun, ChinaHand, foot, and mouth disease (HFMD) is a common viral illness affecting infants and children that is usually caused by Coxsackievirus A16 (CVA-16). To diagnose HFMD, we developed a method for rapid detection of CVA-16 based on reverse transcription-polymerase spiral reaction (RT-PSR). We used two pairs of primers that specifically recognize the conserved sequences of VP1 coding region of CVA-16, and template RNA was reverse transcribed and amplified in a single tube under isothermal conditions, total reaction time could be reduced to less than 40 min. The detection limit of this method was between 2.4 × 102 and 2.4 × 101 copies/μl with excellent specificity. To test the clinical applicability of the method, 40 clinical stool samples were analyzed using RT-PSR and quantitative reverse transcription-polymerase chain reaction, and comparison showed that the coincidence rate was 100%. Compared with other similar detection methods, RT-PSR requires less time, simpler operation, and lower cost. These results prove that our novel, simple, and reliable isothermal nucleic acid testing assay has potential application for clinical detection of CVA-16.https://www.frontiersin.org/article/10.3389/fmicb.2020.00734/fullhandfootand mouth diseaseCoxsackievirus A16polymerase spiral reactionisothermal nucleic acid testing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shiyu He Yanzhi Huang Yanling Zhao Bo Pang Lixue Wang Liwei Sun Haoyan Yu Juan Wang Juan Li Xiuling Song Hui Li |
spellingShingle |
Shiyu He Yanzhi Huang Yanling Zhao Bo Pang Lixue Wang Liwei Sun Haoyan Yu Juan Wang Juan Li Xiuling Song Hui Li A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease Frontiers in Microbiology hand foot and mouth disease Coxsackievirus A16 polymerase spiral reaction isothermal nucleic acid testing |
author_facet |
Shiyu He Yanzhi Huang Yanling Zhao Bo Pang Lixue Wang Liwei Sun Haoyan Yu Juan Wang Juan Li Xiuling Song Hui Li |
author_sort |
Shiyu He |
title |
A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease |
title_short |
A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease |
title_full |
A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease |
title_fullStr |
A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease |
title_full_unstemmed |
A Reverse Transcription-Polymerase Spiral Reaction (RT-PSR)-Based Rapid Coxsackievirus A16 Detection Method and Its Application in the Clinical Diagnosis of Hand, Foot, and Mouth Disease |
title_sort |
reverse transcription-polymerase spiral reaction (rt-psr)-based rapid coxsackievirus a16 detection method and its application in the clinical diagnosis of hand, foot, and mouth disease |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-05-01 |
description |
Hand, foot, and mouth disease (HFMD) is a common viral illness affecting infants and children that is usually caused by Coxsackievirus A16 (CVA-16). To diagnose HFMD, we developed a method for rapid detection of CVA-16 based on reverse transcription-polymerase spiral reaction (RT-PSR). We used two pairs of primers that specifically recognize the conserved sequences of VP1 coding region of CVA-16, and template RNA was reverse transcribed and amplified in a single tube under isothermal conditions, total reaction time could be reduced to less than 40 min. The detection limit of this method was between 2.4 × 102 and 2.4 × 101 copies/μl with excellent specificity. To test the clinical applicability of the method, 40 clinical stool samples were analyzed using RT-PSR and quantitative reverse transcription-polymerase chain reaction, and comparison showed that the coincidence rate was 100%. Compared with other similar detection methods, RT-PSR requires less time, simpler operation, and lower cost. These results prove that our novel, simple, and reliable isothermal nucleic acid testing assay has potential application for clinical detection of CVA-16. |
topic |
hand foot and mouth disease Coxsackievirus A16 polymerase spiral reaction isothermal nucleic acid testing |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00734/full |
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