Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis
The outbreaks of enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) have emerged as an emergency of global health due to its association with fatal encephalitis and subsequent neurogenic pulmonary edema; however, the molecular characteristics and pathological features underlying E...
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doaj-9c9ad63c93e94ebaa91d76982ae9a7332021-06-18T05:52:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-06-011210.3389/fmicb.2021.663019663019Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic AnalysisYuefei Jin0Dong Li1Tiantian Sun2Yue Du3Yanlei Gao4Ronghua Ding5Wangquan Ji6Weiguo Zhang7Weiguo Zhang8Haiyan Yang9Shuaiyin Chen10Guangcai Duan11Guangcai Duan12Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Immunology, Duke University Medical Center, Durham, NC, United StatesDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaDepartment of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou, ChinaHenan Key Laboratory of Molecular Medicine, Zhengzhou University, Zhengzhou, ChinaThe outbreaks of enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) have emerged as an emergency of global health due to its association with fatal encephalitis and subsequent neurogenic pulmonary edema; however, the molecular characteristics and pathological features underlying EV71-associated encephalitis and pulmonary edema remain largely unknown. In this study, we performed a proteomic analysis of fresh brain and lung tissues from EV71-infected mice at 7 days post infection. We detected a perturbed expression of 148 proteins in the brain and 78 proteins in the lung after EV71 expression. Further analysis showed that the dysregulated proteins in the brain are involved in a variety of fundamental biological pathways, including complement and coagulation cascades, innate and adaptive immune responses, platelet activation, and nitrogen metabolism, and those proteins in the lung participate in innate and adaptive immune responses, phagosome, arginine biosynthesis, and hypoxia-inducible factor 1 signaling pathway. Our results suggested that immune activation, complement and coagulation dysfunction, platelet activation, imbalance of nitrogen metabolism, and hypoxia could be involved in the pathogenesis of EV71, which explains the major clinical manifestation of hyperinflammatory status of severe HFMD cases. Our study provides further understanding of the molecular basis of EV71 pathogenesis.https://www.frontiersin.org/articles/10.3389/fmicb.2021.663019/fullenterovirus 71quantitative proteomicscentral nervous systempulmonary edemacomplement and coagulation cascades |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuefei Jin Dong Li Tiantian Sun Yue Du Yanlei Gao Ronghua Ding Wangquan Ji Weiguo Zhang Weiguo Zhang Haiyan Yang Shuaiyin Chen Guangcai Duan Guangcai Duan |
spellingShingle |
Yuefei Jin Dong Li Tiantian Sun Yue Du Yanlei Gao Ronghua Ding Wangquan Ji Weiguo Zhang Weiguo Zhang Haiyan Yang Shuaiyin Chen Guangcai Duan Guangcai Duan Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis Frontiers in Microbiology enterovirus 71 quantitative proteomics central nervous system pulmonary edema complement and coagulation cascades |
author_facet |
Yuefei Jin Dong Li Tiantian Sun Yue Du Yanlei Gao Ronghua Ding Wangquan Ji Weiguo Zhang Weiguo Zhang Haiyan Yang Shuaiyin Chen Guangcai Duan Guangcai Duan |
author_sort |
Yuefei Jin |
title |
Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis |
title_short |
Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis |
title_full |
Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis |
title_fullStr |
Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis |
title_full_unstemmed |
Pathological Features of Enterovirus 71-Associated Brain and Lung Damage in Mice Based on Quantitative Proteomic Analysis |
title_sort |
pathological features of enterovirus 71-associated brain and lung damage in mice based on quantitative proteomic analysis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2021-06-01 |
description |
The outbreaks of enterovirus 71 (EV71)-associated hand, foot, and mouth disease (HFMD) have emerged as an emergency of global health due to its association with fatal encephalitis and subsequent neurogenic pulmonary edema; however, the molecular characteristics and pathological features underlying EV71-associated encephalitis and pulmonary edema remain largely unknown. In this study, we performed a proteomic analysis of fresh brain and lung tissues from EV71-infected mice at 7 days post infection. We detected a perturbed expression of 148 proteins in the brain and 78 proteins in the lung after EV71 expression. Further analysis showed that the dysregulated proteins in the brain are involved in a variety of fundamental biological pathways, including complement and coagulation cascades, innate and adaptive immune responses, platelet activation, and nitrogen metabolism, and those proteins in the lung participate in innate and adaptive immune responses, phagosome, arginine biosynthesis, and hypoxia-inducible factor 1 signaling pathway. Our results suggested that immune activation, complement and coagulation dysfunction, platelet activation, imbalance of nitrogen metabolism, and hypoxia could be involved in the pathogenesis of EV71, which explains the major clinical manifestation of hyperinflammatory status of severe HFMD cases. Our study provides further understanding of the molecular basis of EV71 pathogenesis. |
topic |
enterovirus 71 quantitative proteomics central nervous system pulmonary edema complement and coagulation cascades |
url |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.663019/full |
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