EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes

EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes

<p>This study is focused on the detection of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) mRNA expression levels and protein positions in seven stages of cashmere goat fetus development (45, 55, 65, 75 95, 115, and 135&thinsp;d), with the main goal of investigating the effect...

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Main Authors: Z. Wu, Y. Wang, W. Han, K. Yang, E. Hai, R. Ma, Z. Di, F. Shang, R. Su, R. Wang, Z. Wang, Y. Zhang, J. Li
Format: Article
Language:English
Published: Copernicus Publications 2020-12-01
Series:Archives Animal Breeding
Online Access:https://aab.copernicus.org/articles/63/461/2020/aab-63-461-2020.pdf
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spelling doaj-9cb5245b14664313aab3916f1461c6662020-12-10T13:50:18ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222020-12-016346147010.5194/aab-63-461-2020EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genesZ. Wu0Y. Wang1W. Han2K. Yang3E. Hai4R. Ma5Z. Di6F. Shang7R. Su8R. Wang9Z. Wang10Y. Zhang11J. Li12J. Li13J. Li14College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Chemistry and Life Science, Chifeng University, Chifeng, Inner Mongolia Autonomous Region 024000, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaCollege of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaEngineering Research Center for Goat Genetics and Breeding, Inner Mongolia Autonomous Region, Hohhot, Inner Mongolia Autonomous Region 010018, ChinaKey Laboratory of Mutton Sheep Genetics and Breeding, Ministry of Agriculture, Hohhot, Inner Mongolia Autonomous Region 010018, China<p>This study is focused on the detection of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) mRNA expression levels and protein positions in seven stages of cashmere goat fetus development (45, 55, 65, 75 95, 115, and 135&thinsp;d), with the main goal of investigating the effect of EDA and EDAR on genes related to hair follicle development. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to measure EDA and EDAR expression levels in seven stages of cashmere goat fetus development. Immunohistochemistry (IHC) was used to locate EDA and EDAR in the critical stage of fetal hair follicle development (45–135&thinsp;d). EDA and EDAR expression in fetal fibroblasts and epithelial cells was interfered with by short hairpin RNA (sh-RNA). The results indicated that EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, <span class="inline-formula"><i>β</i></span>-catenin, TGF-<span class="inline-formula"><i>β</i></span>2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly. This study provides a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development.</p>https://aab.copernicus.org/articles/63/461/2020/aab-63-461-2020.pdf
collection DOAJ
language English
format Article
sources DOAJ
author Z. Wu
Y. Wang
W. Han
K. Yang
E. Hai
R. Ma
Z. Di
F. Shang
R. Su
R. Wang
Z. Wang
Y. Zhang
J. Li
J. Li
J. Li
spellingShingle Z. Wu
Y. Wang
W. Han
K. Yang
E. Hai
R. Ma
Z. Di
F. Shang
R. Su
R. Wang
Z. Wang
Y. Zhang
J. Li
J. Li
J. Li
EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
Archives Animal Breeding
author_facet Z. Wu
Y. Wang
W. Han
K. Yang
E. Hai
R. Ma
Z. Di
F. Shang
R. Su
R. Wang
Z. Wang
Y. Zhang
J. Li
J. Li
J. Li
author_sort Z. Wu
title EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
title_short EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
title_full EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
title_fullStr EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
title_full_unstemmed EDA and EDAR expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
title_sort eda and edar expression at different stages of hair follicle development in cashmere goats and effects on expression of related genes
publisher Copernicus Publications
series Archives Animal Breeding
issn 0003-9438
2363-9822
publishDate 2020-12-01
description <p>This study is focused on the detection of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) mRNA expression levels and protein positions in seven stages of cashmere goat fetus development (45, 55, 65, 75 95, 115, and 135&thinsp;d), with the main goal of investigating the effect of EDA and EDAR on genes related to hair follicle development. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to measure EDA and EDAR expression levels in seven stages of cashmere goat fetus development. Immunohistochemistry (IHC) was used to locate EDA and EDAR in the critical stage of fetal hair follicle development (45–135&thinsp;d). EDA and EDAR expression in fetal fibroblasts and epithelial cells was interfered with by short hairpin RNA (sh-RNA). The results indicated that EDA and EDAR were both expressed in the skin tissue in the seven cashmere goat embryo stages. Moreover, EDA and EDAR play an important role in the formation of embryonic placode (Pc). After interfering with EDA and EDAR, the expression of BMP2, BMP4, noggin, <span class="inline-formula"><i>β</i></span>-catenin, TGF-<span class="inline-formula"><i>β</i></span>2, Wnt-10b, and NOTCH1 in fibroblasts and epithelial cells changed significantly. This study provides a theoretical and experimental basis for further studying the molecular regulation mechanism of hair follicle development.</p>
url https://aab.copernicus.org/articles/63/461/2020/aab-63-461-2020.pdf
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