Optimization of Prebiotics and Oxygen Scavengers for Bifidobacterium bifidum BB01 Microcapsules by Response Surface Methodology

• Main Authors: Li Yilin, Shu Guowei, Li Yichao, Liu Yu, Song Yajuan
• Format: Article
• Language: English
• Published: Sciendo 2018-08-01
• Series: Acta Universitatis Cibiniensis. Series E: Food Technology
• Subjects: optimization; encapsulation yield; box-behnken design; viable counts; oxygen scavengers; prebiotics; bifidobacterium bifidum bb01
• Online Access: https://doi.org/10.2478/aucft-2018-0001

The effects of different prebiotics and oxygen scavengers on making the process of xanthan gum and chitosan (XC) Bifidobacterium bifidum BB01 microcapsules were studied by single factor experiment and Plackett-Burman screening test, ascorbic acid, sodium erythorbate and xylo-oligosaccharide had significant effects. Based on the previous studies, the process XC B. Bifidum BB01 microcapsules were further optimized by Box-Behnken model in this study. Response surface analysis showed that the best additive amoumt of ascorbic acid, sodium erythorbate and xylo-oligosaccharide were 3.0%, 2.36% and 4.99%, respectively. The viable counts of B. Bifidum BB01 microcapsules reached to 1.52×1010CFU/g from 1.25×1010 CFU/g, the encapsulation yield reached to 94.88% from 90% under the optimum conditions. It provided the research foundation for the afterward production and exploration of the process XC B. Bifidum BB01 microcapsules.