Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress
Salinity stress is one of the major abiotic stresses that limit agricultural yield. To understand salt-responsive protein networks in soybean seedling, the extracted proteins from seedling roots of two different genotypes (Lee 68 and Jackson) were analyzed under salt stress by two-dimensional polyac...
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doaj-9cbcca4767614bb7bf3872aeab3386662020-11-25T00:31:19ZengElsevierEuPA Open Proteomics2212-96852014-09-014C405710.1016/j.euprot.2014.05.005Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stressHongyu Ma0Liru Song1Zhigang Huang2Yan Yang3Shuang Wang4Zhankui Wang5Jianhua Tong6Weihong Gu7Hao Ma8Langtao Xiao9State Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaHunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, Hunan Province 410128, PR ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaHunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, Hunan Province 410128, PR ChinaAnimal and Plant Introduction and Research Center, Shanghai Agricultural Academy, Shanghai 201106, PR ChinaState Key Laboratory of Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, PR ChinaHunan Provincial Key Laboratory of Phytohormones, Hunan Agricultural University, Changsha, Hunan Province 410128, PR ChinaSalinity stress is one of the major abiotic stresses that limit agricultural yield. To understand salt-responsive protein networks in soybean seedling, the extracted proteins from seedling roots of two different genotypes (Lee 68 and Jackson) were analyzed under salt stress by two-dimensional polyacrylamide gel electrophoresis. Sixty-eight differentially expressed proteins were detected and identified. The identified proteins were involved in 13 metabolic pathways and cellular processes. Proteins correlated to brassinosteroid and gilbberellin signalings were significantly increased only in the genotype Lee 68 under salt stress; abscisic acid content was positively correlated with this genotype; proteins that can be correlated to Ca2+ signaling were more strongly enhanced by salt stress in the seedling roots of genotype Lee 68 than in those of genotype Jackson; moreover, genotype Lee 68 had stronger capability of reactive oxygen species scavenging and cell K+/Na+ homeostasis maintaining in seedling roots than genotype Jackson under salt stress. Since the genotype Lee 68 has been described in literature as being tolerant and Jackson as sensitive, we hypothesize that these major differences in the genotype Lee 68 might contribute to salt tolerance. Combined with our previous comparative proteomics analysis on seedling leaves, the similarities and differences between the salt-responsive protein networks found in the seedling leaves and roots of both the genotypes were discussed. Such a result will be helpful in breeding of salt-tolerant soybean cultivars.http://www.sciencedirect.com/science/article/pii/S2212968514000385SoybeanSalt stressProteomicsMetabolismSalt tolerant and sensitive |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongyu Ma Liru Song Zhigang Huang Yan Yang Shuang Wang Zhankui Wang Jianhua Tong Weihong Gu Hao Ma Langtao Xiao |
spellingShingle |
Hongyu Ma Liru Song Zhigang Huang Yan Yang Shuang Wang Zhankui Wang Jianhua Tong Weihong Gu Hao Ma Langtao Xiao Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress EuPA Open Proteomics Soybean Salt stress Proteomics Metabolism Salt tolerant and sensitive |
author_facet |
Hongyu Ma Liru Song Zhigang Huang Yan Yang Shuang Wang Zhankui Wang Jianhua Tong Weihong Gu Hao Ma Langtao Xiao |
author_sort |
Hongyu Ma |
title |
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
title_short |
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
title_full |
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
title_fullStr |
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
title_full_unstemmed |
Comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
title_sort |
comparative proteomic analysis reveals molecular mechanism of seedling roots of different salt tolerant soybean genotypes in responses to salinity stress |
publisher |
Elsevier |
series |
EuPA Open Proteomics |
issn |
2212-9685 |
publishDate |
2014-09-01 |
description |
Salinity stress is one of the major abiotic stresses that limit agricultural yield. To understand salt-responsive protein networks in soybean seedling, the extracted proteins from seedling roots of two different genotypes (Lee 68 and Jackson) were analyzed under salt stress by two-dimensional polyacrylamide gel electrophoresis. Sixty-eight differentially expressed proteins were detected and identified. The identified proteins were involved in 13 metabolic pathways and cellular processes. Proteins correlated to brassinosteroid and gilbberellin signalings were significantly increased only in the genotype Lee 68 under salt stress; abscisic acid content was positively correlated with this genotype; proteins that can be correlated to Ca2+ signaling were more strongly enhanced by salt stress in the seedling roots of genotype Lee 68 than in those of genotype Jackson; moreover, genotype Lee 68 had stronger capability of reactive oxygen species scavenging and cell K+/Na+ homeostasis maintaining in seedling roots than genotype Jackson under salt stress. Since the genotype Lee 68 has been described in literature as being tolerant and Jackson as sensitive, we hypothesize that these major differences in the genotype Lee 68 might contribute to salt tolerance. Combined with our previous comparative proteomics analysis on seedling leaves, the similarities and differences between the salt-responsive protein networks found in the seedling leaves and roots of both the genotypes were discussed. Such a result will be helpful in breeding of salt-tolerant soybean cultivars. |
topic |
Soybean Salt stress Proteomics Metabolism Salt tolerant and sensitive |
url |
http://www.sciencedirect.com/science/article/pii/S2212968514000385 |
work_keys_str_mv |
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