Vitamin E Supplementation Delays Cellular Senescence In Vitro
Vitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number o...
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Online Access: | http://dx.doi.org/10.1155/2015/563247 |
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doaj-9cd27bb917224b3f9f7eb1a4865589da2020-11-24T23:50:17ZengHindawi LimitedBioMed Research International2314-61332314-61412015-01-01201510.1155/2015/563247563247Vitamin E Supplementation Delays Cellular Senescence In VitroGiorgio La Fata0Nicole Seifert1Peter Weber2M. Hasan Mohajeri3DSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, SwitzerlandDSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, SwitzerlandDSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, SwitzerlandDSM Nutritional Products Ltd., R & D Human Nutrition and Health, P.O. Box 2676, 4002 Basel, SwitzerlandVitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number of cellular divisions. Generally, the most utilized biomarker of senescence is represented by the expression of the senescence associated β-galactosidase (SA-β-gal). We aimed here to study the possible effects of vitamin E supplementation in two different human primary cell types (HUVECs and fibroblasts) during the progression of cellular senescence. Utilizing an unbiased automated system, based on the detection of the SA-β-gal, we quantified cellular senescence in vitro and showed that vitamin E supplementation reduced the numbers of senescent cells during progression of ageing. Acute vitamin E supplementation did not affect cellular proliferation, whereas it was decreased after chronic treatment. Mechanistically, we show that vitamin E supplementation acts through downregulation of the expression of the cycline dependent kinase inhibitor P21. The data obtained from this study support the antiageing properties of vitamin E and identify possible mechanisms of action that warrant further investigation.http://dx.doi.org/10.1155/2015/563247 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Giorgio La Fata Nicole Seifert Peter Weber M. Hasan Mohajeri |
spellingShingle |
Giorgio La Fata Nicole Seifert Peter Weber M. Hasan Mohajeri Vitamin E Supplementation Delays Cellular Senescence In Vitro BioMed Research International |
author_facet |
Giorgio La Fata Nicole Seifert Peter Weber M. Hasan Mohajeri |
author_sort |
Giorgio La Fata |
title |
Vitamin E Supplementation Delays Cellular Senescence In Vitro |
title_short |
Vitamin E Supplementation Delays Cellular Senescence In Vitro |
title_full |
Vitamin E Supplementation Delays Cellular Senescence In Vitro |
title_fullStr |
Vitamin E Supplementation Delays Cellular Senescence In Vitro |
title_full_unstemmed |
Vitamin E Supplementation Delays Cellular Senescence In Vitro |
title_sort |
vitamin e supplementation delays cellular senescence in vitro |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2015-01-01 |
description |
Vitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number of cellular divisions. Generally, the most utilized biomarker of senescence is represented by the expression of the senescence associated β-galactosidase (SA-β-gal). We aimed here to study the possible effects of vitamin E supplementation in two different human primary cell types (HUVECs and fibroblasts) during the progression of cellular senescence. Utilizing an unbiased automated system, based on the detection of the SA-β-gal, we quantified cellular senescence in vitro and showed that vitamin E supplementation reduced the numbers of senescent cells during progression of ageing. Acute vitamin E supplementation did not affect cellular proliferation, whereas it was decreased after chronic treatment. Mechanistically, we show that vitamin E supplementation acts through downregulation of the expression of the cycline dependent kinase inhibitor P21. The data obtained from this study support the antiageing properties of vitamin E and identify possible mechanisms of action that warrant further investigation. |
url |
http://dx.doi.org/10.1155/2015/563247 |
work_keys_str_mv |
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1725479274128867328 |