The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots
The analysis of the effects of cadmium (Cd) on plant cells is crucial to understand defense mechanisms and adaptation strategies of plants against Cd toxicity. In this study, we examined stress-related enzyme activities after one and seven days of Cd application and the ultrastructure of roots of &l...
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doaj-9cdd49a06c5f40889386772fba8b31aa2020-11-25T00:10:07ZengMDPI AGPlants2223-77472019-10-0181041310.3390/plants8100413plants8100413The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea RootsKatarzyna Głowacka0Anna Źróbek-Sokolnik1Adam Okorski2Janusz Najdzion3Department of Plant Physiology, Genetics and Biotechnology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, PolandDepartment of Botany and Nature Protection, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-727 Olsztyn, PolandDepartment of Entomology, Phytopathology and Molecular Diagnostics, University of Warmia and Mazury in Olsztyn, 10-727 Olsztyn, PolandDepartment of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-727 Olsztyn, PolandThe analysis of the effects of cadmium (Cd) on plant cells is crucial to understand defense mechanisms and adaptation strategies of plants against Cd toxicity. In this study, we examined stress-related enzyme activities after one and seven days of Cd application and the ultrastructure of roots of <i>Pisum sativum</i> L. after seven days of Cd treatment (10, 50, 100, and 200 μM CdSO<sub>4</sub>). Our results showed that phenylalanine ammonia-lyase (PAL) activity and the amount of Cd accumulated in the roots were significantly positively correlated with the Cd concentration used in our experiment. However, Cd caused a decrease of all studied antioxidative enzyme activities (i.e., catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX)). The analysis of the ultrastructure (TEM) showed various responses to Cd, depending on Cd concentrations. In general, lower Cd concentrations (50 and 100 μM CdSO<sub>4</sub>) mostly resulted in increased amounts of oil bodies, plastolysomes and the accumulation of starch granules in plastids. Meanwhile, roots treated with a higher concentration of Cd (200 μM CdSO<sub>4</sub>) additionally triggered protective responses such as an increased deposition of suberin lamellae in the endodermal cell walls. This indicates that Cd induces a complex defense response in root tissues.https://www.mdpi.com/2223-7747/8/10/413antioxidative enzymecadmiumphenylalanine ammonia-lyaseplastolysomestarchsuberin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Katarzyna Głowacka Anna Źróbek-Sokolnik Adam Okorski Janusz Najdzion |
spellingShingle |
Katarzyna Głowacka Anna Źróbek-Sokolnik Adam Okorski Janusz Najdzion The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots Plants antioxidative enzyme cadmium phenylalanine ammonia-lyase plastolysome starch suberin |
author_facet |
Katarzyna Głowacka Anna Źróbek-Sokolnik Adam Okorski Janusz Najdzion |
author_sort |
Katarzyna Głowacka |
title |
The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots |
title_short |
The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots |
title_full |
The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots |
title_fullStr |
The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots |
title_full_unstemmed |
The Effect of Cadmium on the Activity of Stress-Related Enzymes and the Ultrastructure of Pea Roots |
title_sort |
effect of cadmium on the activity of stress-related enzymes and the ultrastructure of pea roots |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2019-10-01 |
description |
The analysis of the effects of cadmium (Cd) on plant cells is crucial to understand defense mechanisms and adaptation strategies of plants against Cd toxicity. In this study, we examined stress-related enzyme activities after one and seven days of Cd application and the ultrastructure of roots of <i>Pisum sativum</i> L. after seven days of Cd treatment (10, 50, 100, and 200 μM CdSO<sub>4</sub>). Our results showed that phenylalanine ammonia-lyase (PAL) activity and the amount of Cd accumulated in the roots were significantly positively correlated with the Cd concentration used in our experiment. However, Cd caused a decrease of all studied antioxidative enzyme activities (i.e., catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX)). The analysis of the ultrastructure (TEM) showed various responses to Cd, depending on Cd concentrations. In general, lower Cd concentrations (50 and 100 μM CdSO<sub>4</sub>) mostly resulted in increased amounts of oil bodies, plastolysomes and the accumulation of starch granules in plastids. Meanwhile, roots treated with a higher concentration of Cd (200 μM CdSO<sub>4</sub>) additionally triggered protective responses such as an increased deposition of suberin lamellae in the endodermal cell walls. This indicates that Cd induces a complex defense response in root tissues. |
topic |
antioxidative enzyme cadmium phenylalanine ammonia-lyase plastolysome starch suberin |
url |
https://www.mdpi.com/2223-7747/8/10/413 |
work_keys_str_mv |
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