Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome
<p>Abstract</p> <p>Background</p> <p>Ovarian hyper stimulation syndrome (OHSS) is an iatrogenic complication associated with fertility drugs. It is characterized by increased vascular permeability and substantial fluid shift with accumulation in the body cavity. The pat...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2012-08-01
|
Series: | BMC Cell Biology |
Subjects: | |
Online Access: | http://www.biomedcentral.com/1471-2121/13/23 |
id |
doaj-9cf2a1075c984c438574c75ddcfba3f3 |
---|---|
record_format |
Article |
spelling |
doaj-9cf2a1075c984c438574c75ddcfba3f32020-11-24T21:17:10ZengBMCBMC Cell Biology1471-21212012-08-011312310.1186/1471-2121-13-23Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndromeJin Pei-YinLu Yong-ChaoLi LingHan Qin-Fu<p>Abstract</p> <p>Background</p> <p>Ovarian hyper stimulation syndrome (OHSS) is an iatrogenic complication associated with fertility drugs. It is characterized by increased vascular permeability and substantial fluid shift with accumulation in the body cavity. The pathogenesis of OHSS remains obscure, and no definitive treatments are currently available.</p> <p>Results</p> <p>Using western blot and short-circuit current (Isc) techniques, we investigate the potential coactions of analysis in cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin 1 (AQP1) on the hyper permeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS. The rats develop OHSS symptoms, with the up regulation of both CFTR and AQP1 expression and enhanced CFTR channel activity in peritoneal epithelial cells, can also be mimicked by administration of estrogen, alone in ovariectomized rats. Administration of progesterone suppresses CFTR activity, OHSS symptoms as well as CFTR and AQP1 expression. Besides, AQP1 inhibitor, HgCl<sub>2</sub>, can suppress CFTR channel activity. Therefore, antisera against CFTR or AQP1 to OHSS animals may result in alleviation of the symptom.</p> <p>Conclusion</p> <p>This study confirms the coactions of CFTR and AQP1 play a critical role in the development and progression of increased peritoneal epithelial permeability in severe OHSS. These findings may provide grounds for ameliorating assisted reproduction treatment strategy to reduce the risk of OHSS in <it>in vitro</it> fertilization (IVF).</p> http://www.biomedcentral.com/1471-2121/13/23CFTRAQP1EstrogenOvarian hyper stimulation syndrome |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jin Pei-Yin Lu Yong-Chao Li Ling Han Qin-Fu |
spellingShingle |
Jin Pei-Yin Lu Yong-Chao Li Ling Han Qin-Fu Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome BMC Cell Biology CFTR AQP1 Estrogen Ovarian hyper stimulation syndrome |
author_facet |
Jin Pei-Yin Lu Yong-Chao Li Ling Han Qin-Fu |
author_sort |
Jin Pei-Yin |
title |
Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
title_short |
Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
title_full |
Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
title_fullStr |
Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
title_full_unstemmed |
Co action of CFTR and AQP1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
title_sort |
co action of cftr and aqp1 increases permeability of peritoneal epithelial cells on estrogen-induced ovarian hyper stimulation syndrome |
publisher |
BMC |
series |
BMC Cell Biology |
issn |
1471-2121 |
publishDate |
2012-08-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Ovarian hyper stimulation syndrome (OHSS) is an iatrogenic complication associated with fertility drugs. It is characterized by increased vascular permeability and substantial fluid shift with accumulation in the body cavity. The pathogenesis of OHSS remains obscure, and no definitive treatments are currently available.</p> <p>Results</p> <p>Using western blot and short-circuit current (Isc) techniques, we investigate the potential coactions of analysis in cystic fibrosis transmembrane conductance regulator (CFTR) and aquaporin 1 (AQP1) on the hyper permeability of body cavity peritoneal epithelial cells in the pathogenesis of OHSS. The rats develop OHSS symptoms, with the up regulation of both CFTR and AQP1 expression and enhanced CFTR channel activity in peritoneal epithelial cells, can also be mimicked by administration of estrogen, alone in ovariectomized rats. Administration of progesterone suppresses CFTR activity, OHSS symptoms as well as CFTR and AQP1 expression. Besides, AQP1 inhibitor, HgCl<sub>2</sub>, can suppress CFTR channel activity. Therefore, antisera against CFTR or AQP1 to OHSS animals may result in alleviation of the symptom.</p> <p>Conclusion</p> <p>This study confirms the coactions of CFTR and AQP1 play a critical role in the development and progression of increased peritoneal epithelial permeability in severe OHSS. These findings may provide grounds for ameliorating assisted reproduction treatment strategy to reduce the risk of OHSS in <it>in vitro</it> fertilization (IVF).</p> |
topic |
CFTR AQP1 Estrogen Ovarian hyper stimulation syndrome |
url |
http://www.biomedcentral.com/1471-2121/13/23 |
work_keys_str_mv |
AT jinpeiyin coactionofcftrandaqp1increasespermeabilityofperitonealepithelialcellsonestrogeninducedovarianhyperstimulationsyndrome AT luyongchao coactionofcftrandaqp1increasespermeabilityofperitonealepithelialcellsonestrogeninducedovarianhyperstimulationsyndrome AT liling coactionofcftrandaqp1increasespermeabilityofperitonealepithelialcellsonestrogeninducedovarianhyperstimulationsyndrome AT hanqinfu coactionofcftrandaqp1increasespermeabilityofperitonealepithelialcellsonestrogeninducedovarianhyperstimulationsyndrome |
_version_ |
1726013774171734016 |